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2015, Number 2

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Revista Cubana de Ortopedia y Traumatología 2015; 29 (2)

Characterization of nosocomial isolates of S. aureus purulent samples in an orthopedic hospital in Cuba

Duquesne AA, Castro SN, Monzote LA, Paredes CI, Cabrera ÁC

Language: Spanish
References: 24
Page: 122-131
PDF size: 133.25 Kb.


ABSTRACT

Objective: microbiologically characterize isolates of Staphylococcus aureus recovered from purulent samples to patients with infections associated with health care at "Fructuoso Rodríguez" Teaching Orthopedic Hospital from January to May 2014.
Method: a descriptive study was conducted at "Fructuoso Rodríguez" Teaching Orthopedic Hospital from January to May 2014 in which 134 samples from hospitalized patients were analyzed. Sample processing was performed by conventional microbiological methods and for those samples where S. aureus was recovered, antimicrobial susceptibility by the Kirby Bauer method and E-test was determined for vancomycin.
Results: 41.0% of S. aureus isolates were identified. 52.7% of these were obtained from surgical wounds. It was found that 50.9% of the isolates were meticillinresistant S. aureus. No vancomycin resistant strain was found. The isolates of meticillin-resistant S. aureus were significantly more resistant than S. aureus meticillin sensitive to erythromycin and tetracyclines (tetracycline and doxycycline).
Conclusions: S. aureus was the microorganism most found in the purulent samples studied. In hospital S. aureus isolates predominated in the surgical wound which is in line with the literature reviewed. Half of the studied isolates proved meticillin-resistant S. aureus. All antimicrobials tested except vancomycin, showed higher percentages of meticillin resistance in isolates resistant to meticillin sensitive in which an increase in the hospital stay and cost of care for patients with S. aureus meticillin resistant.


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