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Academia Mexicana de Neurología, A.C.

2009, Number 4

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Rev Mex Neuroci 2009; 10 (4)

Experimental models of neurodegeneration: Restorative treatment and nerve growth factor

Lorigados PL, Pavón FN, Serrano ST, Blanco LL, Almaguer MW, Fernández VI, Macías GR, de la Cuétara BK, Castillo DL, Martínez ML, Robinson AMA, Bergado RJ

Language: Spanish
References: 49
Page: 264-273
PDF size: 252.55 Kb.


ABSTRACT

Objectives: To evaluate the nerve growth factor (NGF) content in three experimental models: hemiparkinsonian rats (6 OHDA), striatal lesion rats (quinolinic acid) and old rats with cognitive deficit; as well as in patient with Alzheimer’s, Parkinson’s and Huntington’s diseases (AD, PD and HD). To analyze how NGF content is affected by the application of different neurorestorative therapies. Material and methods: The content of NGF (serum and central tissue) was measured by means of enzyme immunoassay and in experimental models was evaluated by behavioral methods as rotation induced by D amphetamine and Morri’s task. In each experimental models were evaluated the NGF level after the application of neurorestorative techniques like neural transplantation and the neurotrophic therapy (intracerebral infusion). Results: There is a decreased NGF level in PD and HD patients (p ‹ 0.01). In the PD the NGF amount is related to the state of the diseases. The smaller NGF levels were observed in the initial stadiums of the disease (p ‹ 0.01). We proved that these changes don’t depend on the antiparkinsonian treatment. We also observed a decrement of the NGF levels in all experimental models while an increment (p ‹ 0.05) of NGF in the simultaneous transplant in two nuclei (striatum and subtalamic nucleus) in the pattern of 6 OHDA was observed, as well as in the striatal lesion where a protective effect of the NGF was evidenced in rats treated two days before lesion (p ‹ 0.05). The neurorestorative therapy in aged rats with cognitive deficit showed increase in the levels of NGF (p ‹ 0.001) when was used the combination of the transplant with the NGF intracerebral infusion. Conclusion: There are alterations in the levels of NGF in the PD and HD. The NGF levels are diminished in the experimental models of neurodegeneration and these levels are modifying by the use of neurorestorative techniques, especially in the case that it is combined way the transplant and the trophic therapy.


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