2012, Number 3
<< Back Next >>
Rev Mex Med Repro 2012; 4.5 (3)
Results of a program of IVF with embryo transfer in a day 3 vs day 5
Castillo BJ, Díaz SP, Santos HR, Galache VP, Hernández AS, García VG
Language: Spanish
References: 13
Page: 126-131
PDF size: 88.39 Kb.
ABSTRACT
Background: Whether blastocyst-stage transfer offers any real benefit to infertile couples remains controversial .
Objective: To compare de pregnancy rates of day 3 cleavage
versus day 5 blastocyst fresh non donor embryo transfers in patients under 35 years old.
Material and methods: A prospective non-ramdomized study. A total of 242 consecutive treatment cycles with fresh non donor embryos in patients younger than 35 years old were reviewed. In 132 patients the transfer occurred on day 3 and in 110 patients on day 5. Day 3 transfers fulfilled embryo quality criteria at least 8 cells on the morning of day 3, less than 10% of anucleate fragments).
Results: Demographics and stimulation features between the two groups (day 3
vs day 5) were not significantly different when evaluated: Age (30.4
vs 29.1); time of infertility (4.94
vs 4.61 years) or type of infertility; total doses of FSHr (2422.5
vs 2220.0 IU). There were no significance difference between clinical pregnancy rate (47%
vs 46%) and live birth rate (32%
vs 29%) between day 3 group and in blastocyst day 5 embryo transfers.
Conclusion: In the group of patients under 35 years old, blastocyst stage do not offer better chance of achieving a live delivery than with cleavage-stage.
REFERENCES
Magli MC, Jones GM, Gras L, et al. Chromosome mosaicism in day 3 aneuploid embryos that develop tomorphologically normal blastocysts in vitro. Hum Reprod 2000;15:1781-1786.
Staessen C, Platteau P, Van Assche E, et al. Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomized controlled trial. Hum Reprod 2004;19:2849-2858.
Tsirigotis M. Blastocyst stage transfer: pitfalls and benefits. Too soon to abandon current practice? Hum Reprod 1998;13:3285-3289.
Jones GM, Trounson AO, Lolatgis N, Wood C, et al. Factors affecting the success of human blastocyst development and pregnancy following in vitro fertilization and embryo transfer. Fertil Steril 1998;70:1022-1029.
Racowsky C, Jackson KV, Cekleniak NA, et al. The number of eight-cell embryos is a key determinant for selecting day 3 or day 5 transfer. Fertil Steril 2000;73:558-564.
Blake DA, Proctor M, Johnson NP. The merits of blastocyst versus cleavage stage embryo transfer: a Cochrane review. Hum Reprod 2004;19:795-807.
Papanikolaou E, D’haeseleer E, Verheyen G, et al. Live birth rate is significantly higher after blastocyst transfer than after cleavage-stage embryo transfer when at least four embryos are available on day 3 of embryo culture. A randomized prospective study. Human Reproduction 2005;20:3198-3203.
Munne S, Cohen J. Chromosome abnormalities in human embryos. Hum Reprod Update 1988;4:842-855.
Mellon J, Fluker MR, Yuzpe AA, Kelk DA, et al. Blastocyst transfers improve pregnancy rates for select older patients, but not for those under 37 years old. Fertil Steril 2004;82, Suppl 2;192.
Munne S, Fragouli E, Colls P, et al. Comprehensive chromosome screening indicates that anappropriately designed 12-chromosome FISH test would detect 91% of aneuploid blastocysts. RBMOnline 2009;20:92-97.
Jansen RP, Bowman MC, De Boer KA, et al. What next for preimplantation genetic screening (PGS)? Experience with blastocyst biopsy and testing for aneuploidy. Hum Reprod 2008;23:1476-1478.
Tejera A, Herrero J, De Los Santos MJ, Garrido N, et al. Oxygen consumption is a quality marker for human oocyte competence conditioned by ovarian stimulation regimens. Fert Steril 2011;96(3):618-623.
Cruz M, Gadea B, Garrido N, Kamilla S, et al. Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging. J Assist Reprod Genet 2011;28:569-573.