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Instituto Nacional de Neurología y Neurocirugía

2011, Number 4

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Arch Neurocien 2011; 16 (4)

Locomotor impairment induced by intra-hippocampus microinjection of methylazoxymethanol in male rats in the forced swim test

Saavedra M, Rivadeneyra-Domínguez E, Rodríguez-Landa JF

Language: Spanish
References: 34
Page: 186-192
PDF size: 104.42 Kb.


ABSTRACT

Methylazoxymethanol (MAM) and the non-protein amino acid β-N-methyl-amino-L-alanine (L-BMAA) are neurotoxins contained in cycads. The consumption of cycads seeds produces locomotor impairment and rotational behavior in rats in the forced swim test. The present study investigated the neuroanatomical basis of these impairments. Thirty-two male Wistar rats were randomly divided into four groups: vehicle (1.0µl physiological saline), MAM (10nM), glutamate (10nM) and glutamate- MAM (10nM each). The treatments were microinjected every 24h for 7 days. The behavioral effects were evaluated in the forced swim and open field tests. Results: The vehicle did not modify locomotor activity in the open field test or produce rotational behavior in the forced swim test. Methylazoxymethanol promoted rotational behavior on the first day of treatment an effect that significantly decreased throughout the treatment, and increased sink time. Glutamate gradually increased rotational behavior throughout the treatment and the glutamate-MAM combination produced the greatest number of rotations, which significantly increased throughout the treatment. The MAM, glutamate and glutamate-MAM groups exhibited a significant decrease in the number of neurons in the CA1 and CA3 of the hippocampus compared with vehicle. Conclusion: The hippocampus participates in the expression of the locomotor impairment in rats in the forced swim test, an effect possibly mediated by excitatory effects at the neuronal level.


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