Martínez-Pacheco MM, Saucedo-Luna J, Flores-García A, Martínez-Muñoz RE, Campos-García J

Language: English
References: 48
Page: 23-31
PDF size: 310.04 Kb.

ABSTRACT

Colletotrichum lindemuthianum a hemibiotrophic fungus is the causal agent of anthracnose in Phaseolus vulgaris L. This a complex and peculiar vegetative life cycle with unique cell differentiation and growth process, and a high degree of genetic and metabolic plasticity which contribute with its adaptation under adverse environmental conditions. Therefore, the fungus is able to propagate and to survive easily in its specific host and plant detritus, where extracellular enzymes play an important role in the biopolymers degradation. A source of carbon and other nutrients for this fungus is the large quantity of lignocellulose available in the tequila industry waste, this motivates the thought in the production of sugars from enzymatic degradation of cellulosic waste from the agave as an interesting and inexpensive alternative to obtain products from this solid waste. C. lindemuthianum was subject to screening of cellulases, we founded that this fungus secretes a high level of cellulases in medium containing agave lignocellulosic waste when it was compared with other cellulose fungi producer. The enzymatic activity of these lytic enzymes showed saccharification percentage value of 34.3 at optimal pH and temperature. This fungus is an ideal model to study aspects as development, cell differentiation and production of extracellular enzymes with biotechnological application.

REFERENCES

1. Acosta Rodriguez, I., M.G. Pinon Escobed, M.G. Zavala Paramo, E. López Romero, & H. Cano Camacho. Degradation of cellulose by the bean-pathogenic fungus Colletotrichum lindemuthianum. Production of extracellular cellulolytic enzymes by cellulose induction. Antonie van Leeuwenhoek, 2005; 87: 301-310.

2. Alexopoulos, C.J. & C.W. Mims. 1979. Introductory Mycology. Third Edition. Ed. Wiley.

3. Alzate Marin, A.L., G. Soares Baia, T.J. de Paula Junior, G.A. de Carvalho, E. Gonzalves de Barros & M. Alves Moreira. Inheritance of anthracnose resistance in common bean differential cultivars AB 136. Plant Disease, 1995; 81: 996-998.

4. Anderson, A.J. Extracellular enzymes produced by Colletotrichum lindemuthianum and Helmintosporium maydis during growth on isolated bean and corn walls. Phytopathology, 1978; 68: 1585-1589.

5. Anderson, D.W. & R.L. Nicholson. Characterization of a laccase in the conidial mucilage of Colletotrichum graminicola. Mycologia, 1996; 88: 996-1002.

6. Bailey, J.A., R.J. O´Connell, R.J. Pring & C. Nash. 1992. Infection strategies of Colletotrichum species. pp. 88-120. In: J.A. Bailey & M.J. Jeger (Eds). Colletotrichum: Biology, Pathology and control CAB International, Wallingford.

7. Balardin, R.S., A.M. Jarosz & J.D. Kelly. . Virulence and molecular diversity in Colletotrichum lindemuthianum from South Central, and North America. Phytopathology, 1997; 87: 1184-1191.

8. Basset, M.J. List of genes of Phaseolus vulgaris. Annual Report of the Bean Improvement Cooperative, 1996; 39: 1-19.

9. Bonnen, A.M. & R. Hammerschmidt. Role of cutinolytic enzymes in infection of cucumber by Colletotrichum lagenarun. Physiology and Molecular Plant Pathology, 1989; 35: 475-481.

10. Centis, S., B. Dumas, J. Fournier, M. Marolda & M-T. Esquerré-Tugayé. Isolation and sequence analysis of CLPG1, a gene coding for an endopolygalacturonase of the phytopathogenic Colletotrichum lindemuthianum. Gene, 1996; 170: 125-129.

11. Centis, S., I. Guillas, N. Sejalon, M-T. Esquerre-Tugaye & B. Dumas. Endopolygalacturonase genes from Colletotrichum lindemuthianum. Cloning of CLPG2 and comparison of its expression to that of CLPG1 during saprophytic and parasitic growth of the fungus. Molecular Plant Microbe Interactions, 1997; 10: 769-775.

12. Charrier, A. & H. Bennerot. Contribution al etude des races physiologiques de l´ anthracnose du haricot. Annals of Phytopathology, 1970; 2: 489-506.

13. D ickman, M.B., S.S. Patil & P.E. Kolattukudy. Effect of organophosphorus pesticides on cutinase activity and infection of papaya by Colletotrichum gloesporoides. Physiological Biochemistry 1983; 73: 1209-1214.

14. Dickman, M.B. & S.S. Patil. Cutinase deficient mutants of Colletotrichum gloesporoides are nonpathogenic to papaya fruit. Physiology and Molecular Plant Pathology 1986; 28: 235-243.

15. D illard, H.R. & A.C. Cobb.. Survival of Colletotrichum lindemuthianum in bean debris in New York State. Plant Disease 1993; 77: 1233-1238.

16. Drijfhout, E. & H.J.C. Davis. Selection of a new set of homogeneously reacting bean (Phaseolus vulgaris) differential to differentiate races of Colletotrichum lindemuthianum. Plant Pathology 1989; 38: 391-396.

17. Dufresne, M., J.A. Bailey, M. Dron & T. Langin. clk1, a serin/threonine protein kinase encoding gene, is involved in pathogenicity of C. lindemuthianum on common bean. Molecular Plant Microbe Interactions 1998; 11: 99-108.

18. D umas, B., C. Borel, C. Hebert, J. Maury, C. Jaquet, R. Balsse & M.T. Esquerre-Tugaye. Molecular characterization of CLPT1, a SEC-4 like Rab/GTPase of the phytopathogenic fungus Colletotrichum lindemuthianum which is regulated by the carbon source. Gene 2001; 272: 219-225.

19. Flores Garcia, A. & M.M. Martinez Pacheco. Capacidad celulolítica de Colletotrichum lindemuthianum en la fase saprofitica. Ciencia Nicolaita 2005; 42: 60-72.

20. Green, J.R., N.A. Pain, M.E. Cannell, G.L. Jones, C.P. Leckie, S. Mc-Cready, K. Mendgen, A.J. Mitchell, J.A. Callow and R.J. O’Connell. Analysis of differentiation and development of the specialized infection structures formed by biotrophic fungal plant pathogens using monoclonal antibodies. Canadian Journal of Botany 1995; 73: s408-s417.

21. Habu, N., K. Igarashi, M. Samejima, B. Petersson & K-E. Eriksson. Enhanced production of cellobiose dehydrogenase in cultured of Phanerochaete crysosporium supplemented with bovine calf serum. Biotechnology and Applied Biochemistry 1997; 20:97-102.

22. Heath, M.C. & D. Skalamera. Cellular interaction between plants and biotrophic fungal parasites. In: Tommerup IC, Andrews JH, ed. Academic Press. Advances in Botany Research 1997; 24: 196-225.

23. Hughes, H.B., R. Carzaniga, S.L. Rawling, J.R. Green & R.J. O’Connell. Spore surface glycoproteins of Colletotrichum lindemuthianum are recognized by a monoclonal antibody, which inhibits adhesion to polystyrene. Microbiology 1999; 147: 1927-1936.

24. Hubbeling, N. Resistance in beans to the lambda race of Colletotrichum lindemuthianum. Proc. 19th International Horticultural Congress 1A 1974: 293.

25. Keon, J.P.R., G. Waksman & J.A. Bailey. A comparison of the biochemical and physiological properties of a polygalacturonase from two races of Colletotrichum lindemuthianum. Physiological and Molecular Plant Pathology 1990; 37: 193-206.

26. Kubo, Y., & I. Furusawa. Melanin biosyntheses: Pre-requisite for successful invasion of the plant host by appressoria of Colletotrichum and Pyricularia. In: G.T. Cole & H.C. Hoch (Plenum, Eds). The fungal spore and disease initiation in plants and animals. New York. 1991: 205-218.

27. Mercure, E.W., B. Leite & R.L. Nicholson. Adhesion of ungerminated conidia of Colletotrichum graminicola to artificial hydrophobic surfaces. Physiology and Molecular Plant Pathology 1994; 45: 421- 440.

28. O ’Connell, R.J. Absence of a specialized interface between intracellular hyphae of Colletotrichum lindemuthianum and cells of Phaseolus vulgaris. New Phytopatologist 1987; 107: 725-734.

29. O ’Connell, R.J., N.A. Pain, K.A. Hutchinson, G.L. Jones & J.R. Green. Ultrastructure and composition of the cells surfaces of infection structures formed by the fungal plant pathogen Colletotrichum lindemuthianum. Journal of Microscopy 1996; 181: 204-212.

30. O ’Sullivan, D., P. Tosi, F. Creusot, B.M. Cooke, T.H. Phan, M. Dron & T. Langin. Variation in genome organization of the plant pathogenic fungus Colletotrichum lindemuthianum. Current Genetic 1998; 33: 291-298.

31. Pain, N.A., J.R. Green, G.L. Jones & R.J. O’Connell. Composition and organization of extracellular matrices around germ tubes and appressoria of Colletotrichum lindemuthianum. Protoplasma 1996; 190: 119-130.

32. Pain, N.A., R.J. O’Connell & J.T. Green. A plasma membrane associated protein is a marker for differentiation and polarization of Colletotrichum lindemuthianum appresoria. Protoplasma 1995; 188: 1-11.

33. Pastor Corrales, M.A., M.M. Otoya, A. Molina & S.P. Singh. Resistance to Colletotrichum lindemuthianum isolates from Middle America and Andean South America in different common bean races. Plant Disease 1995; 79: 63-67.

34. Perfect, S.E., R.J. O’Connell, E.F. Green, C. Doering-Saad & J.R. Green. Expression cloning of a fungal proline-rich glycoprotein specific to the biotrophic interface formed in the Colletotrichum-bean interaction. Plant Journal 1998; 15: 273-279.

35. Rodriguez, R.J. & O.C. Yoder. Selectable genes for transformation of the fungal plant pathogen Glomerella cingulata fsp phaseoli (Colletotrichum lindemuthianum). Gene 1987; 54: 73-81.

36. Sánchez García, B.M., A. Flores Oliva, A. Sánchez Arizpe, S. Pineda Rodríguez, G. López Jiménez, S. Fraire Velasquez, E.R. Garrido Ramírez, J.A. Acosta Gallegos, J. Simpson Williamson & R. Rodriguez Gerra. Colletotrichum lindemuthianum pathotypes in Oaxaca and San Luis Potosi, Mexico and resistance in common bean. Agricultura Tecnica de Mexico 2009; 35: 46-57.

37. Schnock, M.G., G.M. Hoffman & J. Kruger. A new physiological strain of Colletotrichum lindemuthianum infecting Phaseolus vulgaris L. Horticultural Science 1975; 10: 40.

38. Sela-Buurlage, M.B., L. Epstein & R.J. Rodriguez. Adhesion of ungerminates Colletotrichum musae conidia. Physiology and Molecular Plant Pathology, 1991; 39: 345-352.

39. Sicard, D., Y. Michelakis, M. Dron & C. Neema. Variability of resistance to Colletotrichum lindemuthianum in the three centers of diversity of common bean of its host Phaseolus vulgaris. Phytopatology 1997; 87: 807-813

40. Tamayo, P.J., M. Otoya & M.A. Pastor-Corrales. Diversidad de los patotipos de Colletotrichum lindemuthianum, el patógeno de la antracnosis, en Río Negro, Antioquia. Fitopatología Colombiana, 1995; 19: 1-6.

41. Templeton, M.D., E.H.A. Rikkerink, S.L. Solon & R.N. Crowhurst. The cloning and molecular characterization of the glyceraldheyde 3-phosphate dehydrogenase encoding gene and cDNA from the plant pathogenic fungus Glomerella cingulata. Gene 1992; 122: 225-230.

42. Tu, J.C., J.W. Sheppard & D.M. Laidlaw. Occurrence and characterization of the epsilon race of bean anthracnose in Ontario. Plant Disease 1984; 68: 69-70.

43. Tsigos, T.Y. & V. Bouriotis. Purification and characterization of chitin deacetylase from Colletotrichum lindemuthianum. Journal of Biological Chemistry 1995; 270: 26286-26291.

44. Van Dyke, C.G. & C.W. Mims. Ultrastructure of conidia, conidum germination and appressorium development in the plant pathogenic fungus Colletotrichum truncatum. Canadian Journal of Botany, 1991; 69: 2455-2467.

45. Wattard, C., D. Kobiler, A. Dinoor & D. Prusky. Pectate lyase of Colletotrichum gloesporoides attacking avocado fruit: cDNA cloning and involvement in pathogenicity. Physiology and Molecular Plant Pathology 1997; 50: 197-212.

46. Wijesundera, R.L.C., J.A. Bailey, R.J.W. Byrde & A.H. Fielding. Cell wall degrading enzymes of Colletotrichum lindemuthianum: their role in the development of bean anthracnose. Physiology and Molecular Plant Pathology 1989; 34: 403-413.

47. Yerkes, W.D. & O.M. Telis. New races of Colletotrichum lindemuthianum in Mexico. Phytopathology 1956; 46: 564-567.

48. Young, D.H. & H. Kauss. Adhesion of Colletotrichum lindemuthianum spores to Phaseolus vulgaris hypocotyls and polystyrene. Applied and Environmental Microbiology 1984; 47: 616-619.