Echegollen-Guzmán A, Torres-Puebla M, Acosta-González RI, Bocanegra-Alonso A, Díaz-Aparicio E, Flores-Gutiérrez GH

Language: Spanish
References: 16
Page: 197-201
PDF size: 76.37 Kb.

ABSTRACT

An immunoenzymatic assay was developed on nitrocellulose paper for diagnosing human and bovine brucellosis, using lipopolysaccharide of Brucella melitenis 16M as antigen. An indirect-ELISA was the gold-standard for calculating the epidemiological sensitivity and specificity and positive and negative predictive values; the results between both tests were analyzed with the kappa test with a confidence level of 95%. The results for the human serum samples were 64.86%, 71.23%, 53.33% y 80%, for clinical sensitivity and specificity and positive and negative predictive values, respectively; there were low agreement between both tests (kappa = 0.343; p ‹ 0.05). The results for bovine serum samples were 29.17%, 87.78%, 38.89% and 82.23%, respectively; showing inadequate agreement between the tests (kappa = 0.187; p ‹ 0.05). Additional surveys are required for improving the immunological and epidemiological features of this immunoenzymatic assay, before it can be considered as an alternative to the diagnosis of brucellosis.

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