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2008, Number 2

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Rev Inst Nal Enf Resp Mex 2008; 21 (2)

Detection and typing of respiratory syncytial virus using nested RT-PCR in patients with acute respiratory infection

Mojica MF, Escobar MF, Escalante MP, Jaramillo CA, Delgado MP

Language: Spanish
References: 17
Page: 92-98
PDF size: 92.56 Kb.


ABSTRACT

Background: Respiratory Syncytial Virus (RSV) is one of the main causes of acute lower respiratory tract infections (ALRTI), and one of the most important causes of morbidity and mortality in infants worldwide. Diagnosis of the entity and specifically, detection and characterization of certain ethiological agents by conventional methods carries difficulties that molecular techniques such as reverse transcription PCR (RT-PCR) pretend to overcome.
Objective: To develop a nested RT-PCR protocol for the detection and typing of RSV in respiratory simples.
Methods: Nasopharyngeal swabs and aspirates from patients with symptoms compatible of ALRTI, that tested positive or negative for RSV by immunofluorescence assay, were used in this study. The molecular technique applied was a RT-PCR followed by a nested PCR using primers for the nucleocapsid gen, that discriminate RSV type A from B by the differential size of the amplicons.
Results: A total of 30 samples were processed, resulting in 2 positives for RSV type A, 16 for RSV type B, and 7 positive to both types; 5 were negative for RSV.
Conclusion: Our results suggest that this RT-PCR protocol represents a quick and effective alternative for the detection and typing of RSV from respiratory samples.


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