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2021, Number 4

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Biotecnol Apl 2021; 38 (4)

Obtention of an enzymatic hydrolyzate of Andean lupin flour (Lupinus mutabilis Sweet) from pineapple bromelain

Navas EVA, López HOD, Morales ADC, Carpio CM

Language: Spanish
References: 21
Page: 4211-4215
PDF size: 417.07 Kb.


ABSTRACT

Hydrolyzates have allowed adding value to foods with a high amount of proteins such as legumes because they improve the bioavailability of their proteins, therefore the purpose of this investigation was to obtain an enzymatic hydrolyzate from andean lupin flour. For which pineapple juice was used as the source of bromelain. Two factors were studied: pH 5.0 and 6.0;and substrate-water ratio 1/10 and 1/20. The prediction equations for the yield and the degree of hydrolysis measured as amino nitrogen percentage were obtained as well as the optimum conditions for the maximum desirability of 87.3 % which were pH 5.0, substrate-water ratio 1/10.3, and 60 min for the hydrolysis time allowed to reach 0.88% of amine nitrogen, from these conditions an scale up for industrial production was performed. The hydrolyzate characterization was carried out by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high performance liquid chromatography, which allowed to conclude that the protein was fractionated in peptides and as a techno-functional property, its solubility improved and the hydrolyzate could be applied as a functional ingredient. chitosan concentration in both strains.


REFERENCES

  1. Drago SR, Luggren PJ, Vioque PJ, Betancur AD, Chel GL, González RJ. Propiedades bioactivas de hidrolizados de gluten de trigo. OmniaScience Monographs, Barcelona. 2013;1(0):83-109.

  2. Razali A, Amin A, Sarbon N. Antioxidant activity and functional properties of fractionated cobia skin gelatin hydrolysate at different molecular weight. Int Food Res J. 2015;22(2):651.

  3. Evangelho JA, Berrios JD, Pinto VZ, Antunes, MD, Vanier NL, Zavareze ER. Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates. Food Sci Technol (Campinas). 2016;36(1):23-7.

  4. Caicedo C, Peralta E, Villacrés E, RiveraM. Poscosecha y mercado de chocho (Lupinusmutabilis Sweet) en Ecuador. BoletínTécnico. INIAP Estación ExperimentalSanta Catalina, Programa Nacional deLeguminosas. 2001;105:37-4.

  5. Thiansilakul Y, Benjakul S, Shahidi F.Antioxidative activity of protein hydrolysatefrom round scad muscle usingalcalase and flavourzyme. J Food Biochem.2007;31(2):266-87.

  6. Lapierre H, Binggeli S, Sok M, Pellerin D,Ouellet D. Estimation of correction factorsto determine the true amino acid concentrationof protein after a 24-hour hydrolysis.J Dairy Sci. 2019;102(2):1205-12.

  7. Aguilera C. Cinética de inactivaciónenzimática y de degradación de saboren función de la temperatura en jugo depiña. Cholula: Universidad de las AméricasPuebla; 2003.

  8. Hernández M, Carvajal C, Márquez M,Báez R, Morris H, Santos R, et al. Obtenciónde preparados enzimáticos a partirde tallos de piña (Ananas comosus) conpotencialidades de uso en la biotecnologíay la medicina. Rev CENIC Cienc Biol.2005;36:12-3

  9. Clavijo D, Portilla M, Parra, A. Cinética yextracción de la bromelina obtenida apartirde la piña (Ananas comosus) provenientede Lebrija-Santander. Ciencia y TecnologíaAlimentaria. 2011(cited 2020 Mar 5); 10(1). Available from: http://revistas.unipamplona.edu.co/ojs_viceinves/index.php/ALIMEN/article/view/99/0

  10. Zhurbenko R, Rodríguez C, Varela A.Método de obtención de base nutritivapara el cultivo de microorganismos y productoobtenido. Patente Cuba. Certificadode Autor No. 22310. Oficina Cubana dela Propiedad Industrial; 2007.

  11. Manzoor Z, Nawaz A, Mukhtar H,Haq I. Bromelain: Methods of extraction,purification and therapeutic applications.Braz Arch Biol Technol. 2016 (cited 2020Mar 5);59. Available from: https://www.scielo.br/j/babt/a/sqJsWgp9PVL9XN3k7R6yT5G/?lang=en&format=html

  12. Kaur T, Kaur A, Grewal R. Kineticsstudies with fruit bromelain (Ananascomosus) in the presence of cysteineand divalent ions. J Food Sci Technol.2015;52:5954-60.

  13. Lago IL, Varela JD, Cáceres FM. La bromelina:una proteasa de interés comercial.CYTA-J Food. 2008;18:4-2.

  14. Carvajal C, Márquez M, Pérez A,Chávez M, Hernández M. Caracterizacióncinética de un preparado semipurificadode bromelina para uso antitumoral. RevCubana Plant Med. 2010;15(2):27-41.

  15. Zhurbenko R, Rodríguez C, Díaz M,Durán A, López O, Viera D. Caracterizaciónde la peptona de soya para elcultivo de microorganismos. Rev CubanaMed Trop. 2006 (cited 2020 Mar5); 58(2). Available from: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07602006000200003

  16. Castel MV. Estudio de las propiedadesfuncionales, tecnológicas y fisiológicas delas proteínas de amaranto. Tesis. Institutode Tecnología de Alimentos, Facultad deIngeniería Química, Universidad Nacionaldel Litoral, Argentina; 2010 (cited 2020 Mar5). Available from: http://bibliotecavirtual.unl.edu.ar:8443/handle/11185/212

  17. Quelal M, Nazate K, Villacrés E, CuaránJ. Obtaining and characterization of a quinoaprotein hydrolyzate Chenopodium quinoaWilld. Enfoque UTE. 2019;10(2):79-89.

  18. Benítez R, Ibarz A, Pagan J. Hidrolizadosde proteína: procesos y aplicaciones. ActaBioquím Clín Latinoam. 2008;42(2):227-36.

  19. Camacho B, Moreno M, García D,Medina C, Sidorovas, A. Caracterización deun hidrolizado proteico enzimático obtenidodel pez caribe colorado (Pygocentrus caribaHumboldt). 2007;32(3):188-94.

  20. Bueno LL, Rincón BN. Aprovechamientode piñas de segunda, para la obtenciónde un zumo de piña comercial. Tesis.Universidad Tecnológica de Pereira. Programade Especialización en ProcesosIndustriales Agroalimentarios; 2016 (cited2020 Mar 5). Available from: http://repositorio.utp.edu.co/dspace/bitstream/handle/11059/7390/664804774B928.pdf?sequence=1

  21. Ivanova N, Khomich L, Perova I, Eller K.Pineapple juice nutritional profile. VoprosyPitaniia. 2019;88(2):73-82.




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