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2018, Número 1

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Biotecnol Apl 2018; 35 (1)


Estabilidad, seguridad e inmunidad protectora de la vacuna Gavac® sometida a estrés térmico

Vargas-Hernández M, Santana-Rodríguez E, Montero-Espinosa C, Sordo-Puga Y, Acosta-Hernández A, Fuentes-Rodríguez Y, Pérez-Pérez D, Oliva-Cárdenas A, González-Ramos E, Duarte CA, Moreira-Rubio A, Sánchez-Ortiz I, Domingo-Puentes M, Leon-Barreras L, Pena W, Suárez-Pedroso M

Idioma: Ingles.
Referencias bibliográficas: 52
Paginas: 1222-1227
Archivo PDF: 382.06 Kb.


RESUMEN

Gavac® es una vacuna comercial para el control de la garrapata bovina (Rhipicephalus (Boophilus) microplus) que emplea la proteína Bm86 como antígeno vacunal. Se fabrica en viales multidosis y se administra en esquemas de dos dosis en los días 0 y 28, seguida de una dosis de refuerzo cada seis meses. Como otras vacunas, Gavac® requiere de condiciones de cadena de frío durante su almacenamiento y transportación, pues las condiciones de estrés térmico pueden afectar su eficacia. Sin embargo, en los viales multidosis, las dosis de vacuna remanentes tras el primer uso pueden conllevar a una pérdida sustancial de producto por el riesgo de inestabilidad. En este trabajo se evaluó la seguridad y eficacia de la vacuna sometida a estrés térmico en viales con un uso,para simular las condiciones de campo. La vacuna remanente en los viales tras una punción e incubados a 37 °C por 14 días no incrementó el número de reacciones adversas en el ganado bovino vacunado, ni alteró las propiedades fisicoquímicas de la emulsion. No se observaron diferencias estadísticamente significativas en los títulos de anticuerpos anti-Bm86 después de siete días de incubación a 37 °C. Además, tras 15 días de incubación a 37 °C, la vacuna mantuvo su capacidad para inducir respuesta protectora de anticuerpos suficiente para afectar los parámetros reproductivos de las garrapatas. Estos resultados demostraron la estabilidad, seguridad, eficacia y robustez de Gavac® bajo condiciones de campo.


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