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Organo Oficial de la Sociedad Mexicana de Urología

2007, Número 2

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Rev Mex Urol 2007; 67 (2)


Expresión del gen DD3PCA3 en cáncer de próstata e hiperplasia prostática benigna. Estudio en el Hospital Central Militar de México

Floriano-Sánchez E, Amador-Ortega J, Castro-Marín M, Torres-Salazar JJ, Ricardos-Espinoza AA, Tavera-Ramirez G, López-Silvestre JC, Aboytes-Velásquez EA

Idioma: Español
Referencias bibliográficas: 38
Paginas: 93-101
Archivo PDF: 314.05 Kb.


RESUMEN

El 70% de los casos de cáncer de próstata se detecta en estadios avanzados, por lo que el gen DD3 PCA3 es un interesante marcador candidato para el diagnóstico y pronóstico, ya que es uno de los más específicos para el cáncer de próstata.
Objetivo. Determinar y analizar la expresión del gen DD3 PCA3 en tejidos con cáncer de próstata e hiperplasia prostática benigna.
Métodos. En este estudio realizamos la cuantificación del mRNA del gen DD3, desarrollando la metodología para determinar la expresión génica usando la trascripción reversa-PCR en tiempo-real (Rotor-Gene 3000).
Resultados. La expresión del gen se determinó en cáncer de próstata y se comparó con hiperplasias benignas, expresándose 30.4 veces más (p‹ 0.0001) en las neoplasias; el promedio de expresión para las neoplasias e hiperplasias fueron de 1745 y 57.4, respectivamente.
Conclusiones. En este estudio encontramos que hay expresión del gen DD3PCA3 significativamente mayor en las neoplasias, en comparación con las hiperplasias, por lo que este gen puede ser utilizado como un marcador temprano tumoral, realizándose en biopsias de pacientes con sospecha de cáncer de próstata.


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