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2006, Number 3

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Rev Inst Nal Enf Resp Mex 2006; 19 (3)

Plasma markers of oxidative stress in a healthy 31-60 year old mexican population

Torres-Ramos YD, Sierra-Vargas MP, Olivares-Corichi IM, Hicks GJJ

Language: Spanish
References: 26
Page: 206-213
PDF size: 113.85 Kb.


ABSTRACT

Introduction: Patients with asthma, COPD, diabetes mellitus, kidney diseases and Alzheimer syndrome, conditions associated to oxidative stress, have modifications and rupture of certain plasma biomolecules. In order to explain properly this findings, basal values in normal individuals of such biomolecules should be known.
Objective: To determine the basal values of some markers of oxidative stress in healthy 31 to 60 year old individuals.
Method: Seventy seven healthy volunteers were classified into group 1, 31 to 40 years, group 2, 41 to 50 years and group 3, 51 to 60 years; the following oxidative stress markers were measured: reactive compounds to tiobarbituric acid (TBARs), predisposition to oxidative stress, determination of carbonil groups, total antioxidative capacity of plasma (TACP) and enzymatic activity of paraoxonase. ANOVA and Bonferroni tests were used; a level of 0.05 was considered significant.
Results: Group 2 and 3 showed significant increment in TBARs (8.462 ± 0.571 vs 10.34 ± 1.23 µ,M TBARs, respectively). In the predisposition to lipoper oxidation, group 3 was more susceptible, due to an increase in RCTA levels (477.0 ± 16.71 µM) in comparison to group 2 (432.3 ± 25.71 µM) and 1 (320.6 ± 28.95 µM). There was no difference in the determination of carbonil groups between the groups. TACP is significantly diminished in group 2 in relation to group 1 (0.950 ± 0.071 vs 0.69 ± 0.068 units, respectively). Paraoxonase’s activity showed a significant increase in group 3 in relation to group 1 (0.119 ± 0.004 vs 0.072 ± 0.007 nmol p-nitrophenol/mg protein, respectively).
Conclusion: Advancing age modifies biomolecular markers of oxidative stress; during the natural aging process, the main damage is to lipids.


REFERENCES

  1. Olivares Corichi IM, Guzmán Grenfell AM, Medina Navarro R, Hicks JJ. Especies reactivas del oxígeno (ERO): Bioquímica inorgánica y biomedicina. En: Hicks JJ, editor. Bioquímica. 2da ed. México: McGraw-Hill; 2006.p. 690-708.

  2. Rahman I, Swarska E, Henry M, Stolk J, MacNee W. Is there any relationship between plasma antioxidant capacity and lung function in smokers and in patients with chronic obstructive pulmonary disease? Thorax 2000;55:189-193.

  3. Droge W. Free radicals in the physiological control of cell function. Physiol Rev 2002;82:47-95.

  4. Hicks-Gómez JJ, Sierra-Vargas MP, Olivares-Corichi IM, Torres-Ramos YD, Guzmán-Grenfell AM. Estrés oxidante en asma. Rev Inst Nal Enf Resp Mex 2005;18:70-78.

  5. MacNee W. Oxidants/antioxidants and COPD. Chest 2000;117(5 Suppl 1):303S-317S.

  6. Yagi K. Simple procedure for specific assay of lipid hydroperoxides in serum or plasma. Methods Mol Biol 1998;108:107-110.

  7. Olivares-Corichi IM, Santillan-Medina R, Valle-Laisequilla, Pindaro A, Hicks JJ. Increase of human plasma antioxidant capacity with a novel formulation of antioxidants. Proc West Pharmacol Soc 2003; 46:45-57.

  8. Aviram M, Rosenblat M, Bisgaier CL, Newton RS, Primo-Parmo SL, La Du BN. Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions. A possible peroxidative role for paraoxonase. J Clin Invest 1998;101:1581-1590.

  9. Hicks JJ. Las lipoproteínas. En: Hicks JJ, editor. Bioquímica. 2da ed. México: McGraw-Hill;2006.p.415-435.

  10. Bin AA, Zhang Q, Lim YK, Fang D, Retnam L, Lim SK. Expression of major HDL-associated antioxidant PON-1 is gender dependent and regulated during inflammation. Free Radic Biol Med 2003;34:824-829.

  11. Lowry OH, Roserbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-275.

  12. Amici A, Levine RL, Stadtman ER. Conversion of amino acids residues in proteins and amino acid homopolymers to carbonyl derivatives by metal-catalyzed oxidation reactions. J Biol Chem 1989;264:3341-3346.

  13. Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarkers of oxidative stress. Clin Chim Acta 2003; 329:23-38.

  14. Sastre J, Pallardo FV, Vina J. Mitochondrial oxidative stress plays a key role in aging and apoptosis. IUBMB Life 2000; 49:427-435.

  15. Halliwell B, Whiteman M. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 2004;142:231-255.

  16. Halliwell B. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994;344:721-724.

  17. McCord JM. The evolution of free radicals and oxidative stress. Am J Med 2000;108:652-659.

  18. Hillestrom PR, Covas MI, Poulsen HE. Effect of dietary virgen olive oil on urinary excretion of etheno-DNA adducts. Free Radic Biol Med 2006;41: 1133-1138.

  19. Roberts CK, Vaziri ND, Wang XQ, Barnard RJ. Enhanced NO inactivation and hypertension induced by a high-fat, refined-carbohydrate diet. Hypertension 2000;36:423-429.

  20. Noakes M, Keogh JB, Foster PR, Clifton PM. Effect of an energy-restricted, high-protein, low-fat diet relative to a conventional high-carbohydrate, low-fat diet on weight loss, body composition, nutritional status, and markers of cardiovascular health in obese women. Am J Clin Nutr 2005;81:1298-1306.

  21. Beal FM. Aging, energy and oxidative stress in neurodegenerative disease. Ann Neurol 1995;38:357-366.

  22. Pauwels RA, Buist AS, Calverley PM, Jenkins CR, Hurd SS, and: GOLD Scientific Committee. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease. NHLBI / WHO Global Initiative for Chronic Obstructive Lung Disease (GOLD) Workshop summary. Am J Respir Crit Care Med 2001;163:1256-1276.

  23. Rahman I, Morrison D, Donaldson K, MacNee W. Systemic oxidative stress in asthma, COPD, and smokers. Am J Respir Care Med 1996;154(4 Pt 1):1055-1060.

  24. Witko-Sarsat V, Friedlander M, Capeillere-Blandin C, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int 1996; 49:1304-1313.

  25. Rajesh M, Sulochana KN, Coral K, et al. Determination of carbonyl group content in plasma proteins as a useful marker to assess impairment in antioxidant defense in patients whith Eales’ disease. Indian J Ophthalmol 2004;52:139-144.

  26. Miravitlles M, Murio C, Guerrero T, Gisbert R. Costs of chronic bronchitis and COPD: a 1-year follow-up study. Chest 2003;123:789-791.




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