Allelic and genotypic frequency of human platelet antigens

Isidro Alemán-Ávila; Octavio Martínez-Villegas; Héctor Alfredo Baptista-González; Fany Rosenfeld-Mann; Rocío Trueba-Gómez; Patricia Bouchán-Valencia; Georgina Coeto-Barona; Higinio Estrada-Juárez

2020, Number 1

Rev Hematol Mex 2020; 21 (1)

Allelic and genotypic frequency of human platelet antigens

Alemán-Ávila I, Martínez-Villegas O, Baptista-González HA, Rosenfeld-Mann F, Trueba-Gómez R, Bouchán-Valencia P, Coeto-Barona G, Estrada-Juárez H

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ABSTRACT

Background: The frequency of human platelet alloantigens in a population determines the prevalence of diseases associated with alloimmunization, such as neonatal alloimmune fetal thrombocytopenia.
Objective: To describe the allele frequencies of human platelet alloantigens in a sample of couples.
Material and Method: A cross-sectional, observational and comparative study was made including male-female unrelated couples, selected from August 1st to December 31st, 2014. The human platelet alloantigens (HPA) alleles were determined by PCR-SSP that allows detection of HPA-1a/b, HPA-2a/b, HPA-3a/b, HPA-4a/b, HPA-5a/b, HPA-15a/b.
Results: Fifteen male-female unrelated couples were selected The HPA that showed highest heterozygosity were HPA-3, HPA-4 and HPA-15 with frequencies of 0.500, 0.534 and 0.466, respectively. The HPA-1, HPA-2 and HPA-5 systems showed the most frequent genotype a/a with 0.933, 0.800 and 0.767, respectively. The b/b genotype was identified in the HPA-3, HPA-4, and HPA-15 systems with frequency of 0.167, 0.033 and 0.167, respectively. In five couples (0.334), the incompatibility with platelet antigens was high risk for the development of neonatal alloimmune thrombocytopenia.
Conclusions: The allelic and genotypic frequencies agree with the reports made in other populations. The allelic incompatibility between couples can estimate a risk for development of fetal-neonatal alloimmune thrombocytopenia, pending to evaluate the presence of other concurrent variables.

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