Pérez‑Willis WA, Yance‑Morales M, Pérez‑Soto WS

Language: Spanish
References: 22
Page: 202-209
PDF size: 285.09 Kb.

ABSTRACT

It is known that adipose tissue is composed of adipocytes and stromal vascular fraction. Within this are stem cells derived from adipose tissue, which are being used in various studies; however, at present there is no standardized method for obtaining this fraction, being expensive and in some cases automated equipment is used. In this study we describe a detailed technique of isolation of the stromal vascular fraction that uses basic, economical elements, with optimal results, so that they are accessible to all specialists. We took samples of adipose tissue from the abdominal area of ​​30 female patients 25 to 50 years old, using a lipoaspiration cannula. The samples were subjected to isolation of the stromal vascular fraction with the technique we propose, carrying out a control with a technique called classical, based on the enzymatic method. Then we performed the cell count with the tripan blue staining by means of a Neubauer chamber manually, obtaining an average cell concentration of 427,033 cells/mL of stromal vascular fraction and an average cell viability of 79.6%, as well count time of isolation, which was an average of 75.1 minutes. We conclude that our proposal is a technique of easy application, accessible and economical, with adequate results to perform cell therapy or cell culture, without the need for automated equipment and is a fundamental basis for specialists in reconstructive plastic surgery.

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