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2006, Number S2

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Rev Med Inst Mex Seguro Soc 2006; 44 (S2)

Is volume important when collecting blood from the umbilical cord?

Novelo GBA, Benítez AG, Márquez CS, Sánchez CJA, Franco GE, Arellano OJS, Guerra MA

Language: Spanish
References: 25
Page: 107-110
PDF size: 75.56 Kb.


ABSTRACT

The volume of blood collected from the umbilical cord is an important condition that varies from one collection to another, for which it is necessary to establish the minimum required amount to get an adequate harvest of total nucleated cells, with the purpose of transplant.
Study design and methods: Blood from the umbilical cord was collected during labor or cesarean section in 2005. A quantification of the collected volume was carried out; the unit underwent a volume reduction; total nucleated cells were counted and finally, the unit was cryopreserved. The statistical analysis was made with the statistic software SPSS version 10.0, by making a ROC curve to establish the adequate cut point for the minimum volume of collection needed.
Results: 161 samples of umbilical cord were studied, with an average volume of 89 mL and an average of total nucleated cells of 6.6 x 108 at the end of the process.
A minimum volume of blood collection from the umbilical cord was established as cut point, by a ROC curve, of 70 mL, with a 93 % sensitivity and a 75 % specificity, having as a positive value, a count of 8 x 108 total nucleated cells.
Conclusion: The umbilical cord blood bank decided to establish as the minimum volume of their collections, 70 mL, based on the need to guarantee the minimum volume of total nucleated cells needed for a possible transplant; this value was established based on the results of the umbilical cord program.


REFERENCES

  1. Gluckman E, Broxmeyer HA, Auerbach AD, Friedman HS, Douglas GW et al. Haematopoietic reconstitution in a patient with Fanconi anemia by means of umbilical cord blood from an HLA identical sibling. N Engl J 1989;321:1174-1178.

  2. Cord blood: Establishing a National Haematopoietic Stem Cell Bank Program. Washington, DC: National Academy of Sciences; 2005.

  3. Brunstein and Wagner. Umbilical Cord Blood Transplantation and Banking. Supplemental Material. Annu Rev Med 2006;57:403-407.

  4. Tse W, Laughlin MJ. Umbilical Cord Blood Transplantation: A New Alternative Option. Hematology 2005:377-383

  5. Solves PS, Moraga R, Mirabet V, Lanea L, Soler MA. In utero or Ex utero cord blood collection: an unresolved question. Transfusion 2003;43:1174-1175.

  6. Lasky LC, Lane TA, Miller JP et al. In utero or ex utero cord blood collection: which is better? Trasfusion 2002;42:1261-1267.

  7. Lasky CL, Haley NR, Kuhn BC. Letter to editor. Transfusion 2003;43:1176.

  8. Sparrow LR, Cauch JA, Ramadi LT, Wuagh CM, Kirkland MA. Influence of mode of bird and

  9. collection on WBC yields of umbilicals cord blood units. Transfusion 2002;42:210-215.

  10. Solves P, Perales A, Moraga R, Saucedo E, Soler A, Monleon J. Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units. Acta Haematol 2005;113:241-246.

  11. Ballen K, Hicks J, Dharan B, Ambruso D, Anderson K et al. Racial and ethnic composition of volunteer cord blood donors: comparison with volunteer unrelated marrow donors. Transfusion 2002;42:1279-1284.

  12. Broxmayer HG. Cord Blood: Biology, Immunology, Banking and Clinical Transplantation. Bethesda, Maryland, USA: AABBPRESS; 2004.

  13. Borstein R, Flores A, Montalbán A, del Rey M, de la Serna J, Gilsanz F. A Modified Cord Blood Collection Method Achieves Sufficient Cell Levels for Transplantation in Most Adult Patients. Stem Cells 2005;23:324-334.

  14. Gluckman E, Broxmeyer HA, Auerbach AD, Friedman HS, Douglas GW et al. Haematopoietic reconstitution in a patient with Fanconi anemia by means of umbilical cord blood from an HLA identical sibling. N Engl J 1989;321:1174-1178.

  15. Cord blood: Establishing a National Haematopoietic Stem Cell Bank Program. Washington, DC: National Academy of Sciences; 2005.

  16. Brunstein and Wagner. Umbilical Cord Blood Transplantation and Banking. Supplemental Material. Annu Rev Med 2006;57:403-407.

  17. Tse W, Laughlin MJ. Umbilical Cord Blood Transplantation: A New Alternative Option. Hematology 2005:377-383

  18. Solves PS, Moraga R, Mirabet V, Lanea L, Soler MA. In utero or Ex utero cord blood collection: an unresolved question. Transfusion 2003;43:1174-1175.

  19. Lasky LC, Lane TA, Miller JP et al. In utero or ex utero cord blood collection: which is better? Trasfusion 2002;42:1261-1267.

  20. Lasky CL, Haley NR, Kuhn BC. Letter to editor. Transfusion 2003;43:1176.

  21. Sparrow LR, Cauch JA, Ramadi LT, Wuagh CM, Kirkland MA. Influence of mode of bird and collection on WBC yields of umbilicals cord blood units. Transfusion 2002;42:210-215.

  22. Solves P, Perales A, Moraga R, Saucedo E, Soler A, Monleon J. Maternal, Neonatal and Collection Factors Influencing the Haematopoietic Content of Cord Blood Units. Acta Haematol 2005;113:241-246.

  23. Ballen K, Hicks J, Dharan B, Ambruso D, Anderson K et al. Racial and ethnic composition of volunteer cord blood donors: comparison with volunteer unrelated marrow donors. Transfusion 2002;42:1279-1284.

  24. Broxmayer HG. Cord Blood: Biology, Immunology, Banking and Clinical Transplantation. Bethesda, Maryland, USA: AABBPRESS; 2004.

  25. Borstein R, Flores A, Montalbán A, del Rey M, de la Serna J, Gilsanz F. A Modified Cord Blood Collection Method Achieves Sufficient Cell Levels for Transplantation in Most Adult Patients. Stem Cells 2005;23:324-334.




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