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ISSN 1561-3054 (Electronic)

2018, Number 3

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Rev Cubana Med Trop 2018; 70 (3)

Sensitivity of different larval stages of Aedes albopictus (S) (Diptera: Culicidae) to infection by two species of mermithids nematodes in laboratory conditions

Menéndez DZ, García GI, Hernández CN, González RA, Companioni IA, Berovides AV

Language: Spanish
References: 30
Page: 83-91
PDF size: 252.90 Kb.


ABSTRACT

Introduction: The combination of safe insecticides for the environment, breeding sites´ reduction and biological control is proposed as a promising approach for Culicids control. The enthomoparasites nematodes Romanomermis iyengari and Strelkovimermis spiculatus have been used successfully in laboratory and field studies, but haven't been evaluated yet in Cuba against Aedes albopictus larvae. This mosquito species constitutes a vector of dengue, chikungunya, zika and other arboviruses.
Objective: To determine the sensitivity of three larval stages ofAe. albopictus to the infection of R. iyengari and S. spiculatus in laboratory conditions.
Methods: A dose of 7: 1 (preparasitics/larva) was applied to 100 larvae of Ae. albopictus in different stages of development (I, II and III). 96 hours after the post-infection, 30 larvae were taken at random and dissected to determine the intensity and frequency of the infection. Each Ae. Albopictus larval stage had twelve replicas by stages and controls were made to them.
Results: S. spiculatus showed higher frequency of infection in the larval stages studied (97.22 %, 96.66 % and 73.33 %) than R. iyengari (90.27 %, 83.61 % and 57.22 %), while the intensity of infection by stages was 2.54; 2.44 and 1.55 for S.spiculatus and 2.39; 2.46 and 1.97 for R. iyengari.
Conclusions: Although S. spiculatus was superior to R. iyengari, with higher values of infection frequency in the three stages, both species proved to be good candidates as bio-control agents of Ae. albopictus and subsequent studies are recommended before use in natural conditions.


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