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2019, Number 1

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Rev Mex Ortodon 2019; 7 (1)

Effects of micro-vibration at 30 Hz on IL-17 and RANKL expression in in vitro mouse skull-derived osteoblasts and bone marrow-derived osteoclasts

García LS, Villanueva ARE, Pérez VJL, Juárez NLI, Paéz Arenas A, Massó LF, Meikle MC

Language: Spanish
References: 27
Page: 24-32
PDF size: 240.50 Kb.


ABSTRACT

Introduction: Micro-vibration therapy in patients with osteoporosis has been shown to induce remodeling in the outer cortical bone perhaps due to inhibition of the osteoclastic activity. Objective: We assessed the effects of exposure to micro-vibration for 20 minutes at 30 Hz in osteoblastic and osteoclastic cells to determine the expression of IL-17 and RANKL. Material and methods: Osteoblasts derived from the skull and osteoclasts derived from tibia stem cells of a Balb/c strain mouse were cultured in 24-well plates (Gybco, USA) in the following culture media, respectively: DMEM F-12 (ATCC, USA) and α-MEM (ATCC, USA), adding 0.5 µL/mL of 1α, 25-Dihydroxivitamin D3 (Sigma-Aldrich, USA) to the latter. Both culture media were supplemented with 10% fetal bovine serum (Gybco, USA), 1% of antibiotics and antifungals. Incubation (NuAire, USA) was done in an atmosphere of 5% CO2, 95% humidity at 37 oC. Cultured cells were subsequently subjected to micro-vibrations (0.25 N, 30 Hz) with AcceleDent (Ortho Accel, USA) for 20 minutes. Before and after exposure to the vibrations 500 µL of supernatant of both cultured cells were collected and stored in an ultra-low temperature freezer at -70 oC (Thermo, USA) to later determine IL-17 and RANKL expression (Peprotech, Rocky Hill, NJ, USA) by ELISA test. Results: Micro-vibration produced a statistically significant upregulation of IL-17 and downregulation of RANKL in both cultured osteoblasts and osteoclasts in the experimental group compared with the control group. Conclusion: Micro-vibration at 30 Hz for 20 minutes applied to in vitro cultures of osteoblastic and osteoclastic cells produces soluble factors related to osteoclastic maturation and inhibition.


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