Wong PI, Somontes SD, Rodriguez RF, Morán VR, González FN, Pérez HC, Paneque DY

Language: English
References: 0
Page: 4301-4304
PDF size: 301.55 Kb.

ABSTRACT

Three methods are presented, to evaluate the nematicidal activity of the bioproduct HeberNem® against eggs and larvae of Meloidogyne spp. They are a tool to determine the nematicidal effect of formulations of bioproducts composed of microorganisms that affect the life cycle of the nematodes. The first method or in vitro test allows determining the percentage of inhibition of egg hatching with a coefficient of variation lower than 5 %, and the percentage of reduction larvae survival vs. control with a coefficient of variation lower than 15 %. The second method allows calculating the concentration of microorganisms capable of producing hydrogen sulfide during the product’s development studies. And the third method consists of an essay in 1 L pots containing a 1:1 (v/v) peat-sand mixture indicator plants, to determine the technical effectiveness of the product against eggs and larvae of Meloidogyne spp. Five weeks after sowing, the degree of root infestation is determined and the effectiveness of each treatment is calculated with respect to the untreated control. The HeberNem® formulations tested showed greater than 90 % egg hatching inhibition and 52 % larval survival reduction for the 5 × 10⁶ c.f.u./mL concentration. In the pot trial, the technical effectiveness obtained was greater than 50 % with respect to the control.