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2017, Number 3

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Rev Hematol Mex 2017; 18 (3)

Deoxyribonucleic acid index in patients with acute lymphoblastic leukemia as tool for the classification of risk of early relapse

Parra-Ortega I, Naìjera-Martiìnez N, Mendoza-García E, Vega-Cardelas JM, Hernaìndez-Echaìurregui G, Loìpez-Martiìnez B, Vilchis-OrdonÞez A

Language: Spanish
References: 23
Page: 114-126
PDF size: 829.66 Kb.


ABSTRACT

Background: Deoxyribonucleic acid index (DNA) has close correlation with another clinical settings as forecast factor in children acute lymphoblastic leukemia (ALL). It has been described that hypodiploid DNA index is related to high risk stratification unlike hyperdiploid DNA index.
Objetive: To assess the clinical usefulness of quantification of DNA index for the correct classification of risk of early relapse.
Material and Method: Flow cytometry of bone marrow aspirates (BMA) was performed to determine DNA index in mononuclear cells (MNCs) in children with acute lymphoblastic leukemia (ALL).
Results: Among 27 ALL BMA, 44% were male. According to EGIL classification cell lineage and stage of maturation determined lymphoblastic leukemia identification: ALL corresponded to Pro B (4%), Pre B (30%) and common Pre B (62%), bifenotypic leukemia’s (4%). Hypodiploid DNA index corresponded to 22%, hyperdiploid corresponded to 33.3%.
Conclusion: DNA index is a powerful tool to classify children with ALL according to risk of early relapse, risk stratification (high or standard) modifies treatment schedule. It is important that every diagnostic laboratory develops all the battery on methodologies to classify accurately children with ALL.


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