Gómez SJ, Talamás RP, Aguirre GM

Language: Spanish
References: 20
Page: 4-12
PDF size: 862.93 Kb.

ABSTRACT

Protein phosphatases are enzymes that dephosphorylate proteins in tyrosine and serine/threonine residues, and are involved in various cellular processes, whose functions have been best characterized in eukaryotic cells. However, in the last two decades these enzymes have been identified and characterized in infectious microorganisms such as parasites. It has been found that certain parasite phosphatases maintain conserved functions similar to those of higher eukaryotes, whereas other phosphatases have unique structural and/or functional characteristics, which make them candidates for drug development or as diagnostic markers. This paper encompasses the knowledge generated to date, about the role of these enzymes in parasites of medical and veterinary importance.

REFERENCES

1. Moorhead G, Trinkle-Mulcahy L, Ulke-Lemee A (2007) Emerging roles of nuclear protein phosphatases. Nature Reviews 8:234-244. [ Links ]

2. Kutuzov MA, Andreeva AV (2012) Prediction of biological functions of Shewanella-like protein phosphatases (Shelphs) across different domains of life. Funct Integr Genomics 12(1):11-23. [ Links ]

3. Kutuzov MA, Andreeva AV (2008) Protein Ser/Thr phosphatases of parasitic protozoa. Mol Biochem Parasitol 161:81-90. [ Links ]

4. Jan G, Delorme V, Saksouk N, Abrivard M, Gonzalez V, Cayla X, Hakimi MA, Tardieux I (2009) A Toxoplasma type 2C serinethreonine phosphatase is involved in parasite growth in the mammalian host cell. Microbes Infect. 11(12):935-945. [ Links ]

5. Daher W, Cailliau K, Takeda K, Pierrot C, Khayath N, Dissous C, Capron M, Yanagida M, Browaeys E, Khalife J (2006) Characterization of Schistosoma mansoni Sds homologue, a leucinerich repeat protein that interacts with protein phosphatase type 1 and interrupts a G2/M cell-cycle checkpoint. Biochem J 395(2):433-441. [ Links ]

6. Andreeva AV, Kutuzov, M (2008) Protozoan protein tyrosine phosphatases. Int J Parasitol 8:1279-1295. [ Links ]

7. Lountos GT, Tropea JE, Waugh DS (2013) Structure of the Trypanosoma cruzi protein tyrosine phosphatase TcPTP1, a potential therapeutic target for Chagas' disease. Mol Biochem Parasitol 187(1):1-8. [ Links ]

8. Wimmer M, Schmid B, Tag C, Hoffer H (1998) Ascaris suum:Protein Phosphotyrosine Phosphatases in Oocytes and Developing Stages. Exp Parasitol 88(2):139-145. [ Links ]

9. Lauwaet T, Davids B, Torres-Escobar A, Birkeland S, Cipriano M, Preheim S, Palm D, Svard S, McArthur A, Gillin F (2007) Protein phosphatase 2A plays a crucial role in Giardia lamblia differentiation. Mol Biochem Parasitol 152(1):80-89. [ Links ]

10. Campbell B, Rabelo E, Hofmann A, Hu M, Gasser R (2010) Characterization of a Caenorhabditis elegans glc seven-like phosphatase (gsp) orthologue from Haemonchus contortus (Nematoda). Mol Cell Probes. 24(4):178-189. [ Links ]

11. Deng C, Yu X, Li X, Sun J, Wang L, Wang X, Chen W, Hu X, Wu Z (2012) Molecular expression and characterization of a novel protein phosphatase 2A gene from Clonorchis sinensis. Parasitol Res 110(5):1951-1957. [ Links ]

12. Yi K, Wang X, Emmett, M., Marshall, A., Stewart, M. y Roberts, T (2009) Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm. Mol Biol Cell 20(14):3200-3208. [ Links ]

13. Prestes E, Bayer-Santos E, Hermes P, Marques T, Wagner G, Umaki A, Perdigao S, Bordignon J, Steindel M y Grisard C (2012) Trypanosoma rangeli protein tyrosine phosphatase is associated with the parasite's flagellum. Mem Inst Oswaldo Cruz 107(6): 713-719. [ Links ]

14. Naderer T, Dandash O, McConville MJ (2011) Calcineurin is required for Leishmania major stress response pathways and for virulence in the mammalian host. Mol Microbio 80(2):471-480. [ Links ]

15. Rathaur S, Rai R, Srikanth E, Srivastava S (2009) Setaria cervi dual specific phosphatase: characterization and its effect on eosinophil degranulation. Parasitology 136:895-904. [ Links ]

16. Singh M, Mukherjee P, Narayanasamy K, Arora R, Sen SD, Gupta S, Natarajan K, Malhotra P (2009) Proteome analysis of Plasmodium falciparum extracellular secretory antigens at asexual blood stages reveals a cohort of proteins with possible roles in immune modulation and signaling. Mol Cell Proteomics. 8(9):2102-2118. [ Links ]

17. Escalona-Montaño AR, Pardavé-Alejandre D, Cervantes-Sarabia R, García-López P, Gutiérrez-Quiroz M, Gutiérrez-Kobeh L, Becker-Fauser I, Aguirre-García MM (2010) Leishmania mexicana promastigotes secrete a protein tyrosine phosphatase. Parasitol Res 107(2):309-315. [ Links ]

18. Patzewitz E, Guttery D, Poulin B, Ramakrishnan C, Ferguson D, Wall R, Brady D, Holder A, Szoor B and Tewari R (2013) An ancient protein phosphatase, SHLP1, is critical to microneme development in Plasmodium ookinetes and parasite transmission. Cell Rep 3(3):622-629. [ Links ]

19. Guttery D, Poulin B, Ferguson D, Szoor B, Wickstead B, Carroll P, Ramakrishnan C, Brady D, Patzewitz E, Straschil U, Solyakov L, Green J, Sinden R, Tobin A, Holder A y Tewari R (2012) A unique protein phosphatase with kelch-like domains (PPKL) in Plasmodium modulates ookinete differentiation, motility and invasion. PLoS Pathog 8(9):e1002948. [ Links ]

20. Balderas-Rentería I, García-Lazaro J, Carranza-Rosales, Morales-Ramos, Galan W, Muñoz-Espinosa (2007) Transcripcional upregulation of genes related to virulence activation in Entamoeba histolytica. Arch Med Res 38(4):372-379. [ Links ]