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ISSN 0864-0289 (Print)

2016, Number 3

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Rev Cubana Hematol Inmunol Hemoter 2016; 32 (3)

Isolation and characterization of mesenchymal cells from adipose tissue

Galván CJA, Miranda RA, de León DJ, Macías AC, Baganet CA, Rondón CT, González IAI, Socarras FBB, Bencomo HA, Rivero JRA, Hernández RP

Language: Spanish
References: 49
Page: 375-387
PDF size: 229.95 Kb.


ABSTRACT

Introduction: There is growing scientific interest in the therapeutic potential of stem cells derived from adipose tissue (ADSCs). These cells are abundant in adipose tissue, are readily available and have a high potential fordifferentiation into specialized cell lineages including adipocytes, osteocytes, chondrocytes, myocytes, cardiomyocytes, tenocyte, endothelial cells and neurons. The aim of this work was to isolate, cultivate andcharacterize ADSCs.
Methods: human adipose precursor cells were obtained from abdominal subcutaneous tissue. Cells were enzymatically separated from the tissue and decanted by centrifugation, cultured and finally analyzed.
Results: The ADSCs were able to replicate in our culture conditions. The cells maintained their phenotype in different passages throughout the study period, confirming its feasibility for in vitro culture. Also the ADSCs were induced to adipogenic and osteogenic differentiation, verifying its potential as mesenchymal stem cells in vitro. The flow cytometric study showed that these cells expressed CD73, CD90 and CD105 (markers of mesenchymal cells) and they were negative for CD34 and CD45 (hematopoieticcell markers). The ADSCs were able to inhibit in vitro the proliferation of T cells.
Conclusions: It is possible to obtain ADSCs by in vitro cultivation without adipogenic induction, maintaining its functional integrity and high proliferation; this cell could be an important tool for the cellular therapy in our country.


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