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2016, Number 3

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Rev Mex Patol Clin Med Lab 2016; 63 (3)

Implementation of the Foldscope as a device for cervical cancer diagnosis: A verification and evaluation study

Moya-Salazar J, Bruno M, Rojas-Zumaran V, Bhamla S, Prakash M

Language: Spanish
References: 27
Page: 141-147
PDF size: 808.99 Kb.


ABSTRACT

Objective: To implement, monitor and evaluate the implementation of Foldscope in the screening of cervical cancer in conventional exfoliative cytology. Material and methods: An exploratory, quasi-experimental double-blind research was conducted in Prakash Lab, Department of Bioengineering at Stanford University during May 2016. We selected 10 slides of cervical cytology, four negative for intraepithelial lesion or malignancy (NLIM) and six with cervical uterine abnormalities. The cytological characteristics of the smears were evaluated with both Foldscope and optical microscopy with 10x and 40x magnification lenses. Microphotography, an image station tool and image projection were used. Statistical analysis was performed using IBM SPSS. Results: From the comparison of images between the optical microscope and Foldscope, the same cytological features were found in the cells of both NLIM and preneoplastic or neoplastic lesions (p ‹ 0.001). However, the Foldscope evidenced a clear lack of clarity around the focal point and constraints in focus, which made necessary, more often than not, the use of the image station and image projection. Conclusions: Foldscope is an extraordinary tool for the diagnosis of cervical cancer; despite its obvious limitations, it is able to receive improvements to be made a cost-effective device exclusively for cancer diagnosis.


REFERENCES

  1. Curtis H, Barnes N. Biología. Buenos Aires: Editorial Medica Panamericana; 1993.

  2. Cohen M. What is cell theory? Shaping modern science. Canada: Crabtree Pub.; 2011.

  3. Parker V. Antony van Leeuwenhoek. Bull Med Libr Assoc. 1965; 53 (3): 442-447.

  4. Solomon E, Berf L, Martin D, Villee C. Biología. México: McGraw-Hill Internacional; 1992.

  5. Cybulski JS, Clements J, Prakash M. Foldscope: origami-based paper microscope. PLoS One. 2014; 9 (6): e98781.

  6. Basen-Engquist K, Paskett ED, Buzaglo J, Miller SM, Schover L, Wenzel LB et al. Cervical cancer behavioral factors related to screening, diagnosis, and survivors’ quality of life. Cancer. 2003; S98 (9): 2009-2014.

  7. Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C et al. GLOBOCAN 2012 v1.0, Cancer incidence and mortality worldwide: IARC, Cancer Base No. 11 [Internet]. Lyon: International Agency for Research on Cancer; 2013.

  8. Moya SJ, Pio DL. Prevalence of cervical-uterine abnormalities associated with poverty levels at “Hospital Nacional Docente Madre-Niño San Bartolome” between 2011-2013. Rev Invest Univ Norbert Wiener. 2014; 3 (1): 89-99.

  9. Fernández de Casadevante V, Gil-Cuesta J, Cantarero-Arévalo L. Determinants in the uptake of the human papillomavirus vaccine: a systematic review based on European studies. Front Oncol. 2015; 5 (141): 1-13.

  10. Watson M, Saraiya M, Benard V, Coughlin SS, Flowers L, Cokkinides V et al. Burden of cervical cancer in the United States, 1998-2003. Cancer. 2008; 113: 2855-2864.

  11. Koss LG. The Papanicolaou test for cervical cancer. A triumph or a tragedy. JAMA. 1989; 261 (5): 737-743.

  12. Papanicolaou GN. A new procedure for staining vaginal smears. Science. 1942; 95 (2469): 438-439.

  13. Rojas-Zumaran V, Moya-Salazar J. Validation of the ecological Papanicolaou stain for cervical cancer diagnosis. Acta cytol. In pres.

  14. Moya-Salazar J, Rojas-Zumaran V, Torres-Martínez R, Rosas-Vargas L. Calidad de los extendidos cervicouterinos dentro de la coloración de Papanicolaou para el cribado de cáncer cervical en Lima, Perú. Rev Esp Patol. 2016; 49: 7-18.

  15. Nayar R, Wilbur DC. The Bethesda system for reporting cervical cytology. Definitions, criteria, and explanatory notes. 3rd. ed. Cham: Springer; 2015.

  16. Acuña CR, Falla PA, Romaní RM. Evaluación de la técnica de tinción rápida Cytocolor versus la tinción Papanicolaou en citología cérvico-vaginal [Tesis]. Lima: Universidad Nacional Federico Villareal. Facultad de Tecnología Médica; 2006.

  17. Moya-Salazar J, Rojas-Zumaran V. Environmental Performance of xylene, hydrochloric acid and ammonia solution during Pap stain for diagnosing cervical cancer. J Health Pollution. 2016; 6 (11): 58–65.

  18. Saslow D, Solomon D, Lawson HW et al. American Cancer Society, American Society for Colposcopy and Cervical Pathology, and American Society for Clinical Pathology screening guidelines for the prevention and early detection of cervical cancer. CA Cancer J Clin. 2012; 62 (3): 147-172.

  19. Prakash M. Focuslocking, field of view locking and using ambient light (table lamp) as illumination [Article Online]. [Fecha de acceso: 07-06-16] Available in: https://microcosmos.foldscope.com/?p=16093

  20. Salvetto M, Sandiford P. External quality assurance for cervical cytology in developing countries, experience in Peru and Nicaragua. Acta Cytol. 2004; 48: 23-31.

  21. Alonso de Ruiz P, Lazcano PE, Hernández AM. Cáncer cervicouterino. Diagnóstico, prevención y control. Argentina: Editorial Médica Panamericana; 2000.

  22. Nanda K, McCrory DC, Myers ER, Bastian LA, Hasselblad V, Hickey JD et al. Accuracy of the Papanicolaou test in screening for and follow-up of cervical cytologic abnormalities: a systematic review. Ann Intern Med. 2000; 132: 810-819.

  23. Cooper K. Errors and error rates in surgical pathology: an Association of Directors of Anatomic and Surgical Pathology survey. Arch Path Lab Med. 2006; 130: 607-609.

  24. Longatto-Filho A, Schmitt FC. Cytology education in the 21st century: living in the past or crossing the Rubicon? Acta Cytol. 2010; 54: 654-656.

  25. Ramos-Ortega G, Díaz-Hernández M, Rodríguez-Moctezuma J, Domínguez-Gómez F. Satisfactory cervical cytologic smear against longitudinal exocervical smears. Rev Med Inst Mex Seguro Soc. 2014; 52 (6): 696-703.

  26. Doushkina V, Fleming E. Optical and mechanical design advantages using polymer optics. Advances in Optomechanics. 2009; 74: 24-31.

  27. Leica. La teoría del microscopio. Buffalo NY: Leica Microsystems Inc. Educational and Analytical Division; 2000; Rev A.




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