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Federación Mexicana de Ginecología y Obstetricia, A.C.

2005, Number 11

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Ginecol Obstet Mex 2005; 73 (11)

Serum levels of IL-1Β, IL-6 and TNF-α in infertile patients with ovarian dysfunction

Vital RVS, Téllez VS, Hinojosa CJC, Ortiz RMJ, Chavarría OME, Reyes FA

Language: Spanish
References: 19
Page: 604-610
PDF size: 72.31 Kb.


ABSTRACT

Background: Some cytokines that take part in the ovarian cycle regulation, such as IL-1Β, IL-6 and TNF-α play an important role during growth and development of ovarian follicle. Quantification of some cytokines is promissory in the work-up of ovarian reproductive pathology.
Objective: To determine serum levels of IL-1Β, IL-6 and TNF-α in infertile patients with ovarian dysfunction and to compare them with those found in ovulatory patients.
Patients and methods: We conducted a cross-sectional study in infertile patients with premature ovarian failure, diminished ovarian reserve, chronic anovulation and ovulatory patients that were well-characterized by clinical and hormonal parameters. We determined serum concentrations of IL-1ΒΒ, IL-6 and TNF-α by an ELISA method. Statistics was conducted by using one way ANOVA, Kruskall-Wallis and Pearson correlation analyses.
Results: We studied a total of forty patients. The mean age was 30 years; we did not find significant differences among groups. Overall mean of cytokines concentration was: 13.2 pg/mL for IL-1Β; 8.4 pg/mL for IL-6; and 1.5 pg/mL for TNF-α. We observed a significant difference in the concentration of TNF-a in the premature ovarian failure group when it was compared to the control group. Pearson correlation coefficients were no significant.
Conclusion: A wide range of dispersion of serum cytokines concentration was observed. Serum concentrations of TNF-α in woman with premature ovarian failure were significantly lower than those in the control group. Our findings represent a precedent in the powerful utility of the quantification of these cytokines in reproductive medicine.


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