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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2005, Number 4

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Rev Invest Clin 2005; 57 (4)

CD4 and CD8 T cell response to the rHSP60 from Klebsiella pneumoniae in peripheral blood mononuclear cells from patients with ankylosing spondylitis

Zambrano-Zaragoza F, García-Latorre E, Domínguez-López ML, Cancino-Díaz ME, Burgos-Vargas R, Jiménez-Zamudio L

Language: English
References: 40
Page: 555-562
PDF size: 294.62 Kb.


ABSTRACT

Objective. To determine the processing pathways used by peripheral blood mononuclear cells (PBMC) and present the rHSP60Kp, and the T cell subpopulations involved in the response, in patients with ankylosing spondylitis (AS) Meth-ods. The lymphoproliferative response to the rHSP60Kp in PBMC from 14 HLA-B27+ AS patients and 15 B27- healthy controls was assessed by 3H-TdR incorporation. The processing pathways for the rHSP60Kp were analyzed by 3H-TdR incorporation in fresh PBMC from patients using homologous PBMC preincubated with the antigen and specific inhibi-tors: chloroquine, N-acetyl-L-leucil-L-leucil-L-nor-leucinal (LLnL) or brefeldin A (BFA), fixed with p-formaldehyde (fixed APC). The CD4+/CD8+ T cell subpopulation activated with the antigen was determined by three colours flow cytome-try in PBMC from patients. Results. Eight out of fourteen patients showed positive lymphoproliferative responses to the rHSP60Kp while none of the healthy controls responded (p ‹ 0.012). In five patients S.I. was above 4.0. In these patients lymphoproliferation was lower when chloroquine and LLnL was used and it became negative with BFA, indicating that both pathways are used. CD4+ and CD8+ T cells populations expressed CD69 when activated by the rHSP60Kp. Conclusions. Our results suggest that CD4 and CD8 T cells participate in the response to the rHSP60Kp in B27+ AS patients.


REFERENCES

  1. Burgos-Vargas R, Granados-Arriola J. Ankylosing spondylitis and related diseases in the Mexican Mestizo. Spine-State of the Art Rev 1990; 4: 665-77.

  2. D’Amato M, Fiorillo MT, Carcassi C, Mathieu A, Zuccarelli A, Bitti PP, Tosi R, Sorretino R. Relevance of residue 116 of HLA-B27 in determining susceptibility to ankylosing spondylitis. Eur J Immunol 1995; 25: 3199-201.

  3. Khan MA. Update: the twenty subtypes of HLA-B27. Curr Opin Rheumatol 2000; 12: 235-8.

  4. Saraux A, Guedes C, Allain J, Valls I, Baron D, Youinou P, Le Goff P. HLA-B27 in French patients with rheumatoid arthritis. Scand J Rheumatol 1997; 26: 269-71.

  5. Avakian H, Welsh J, Ebringer A, Entwistle CC. Ankylosing spondylitis, HLA-B27 and Klebsiella pneumoniae II. Cross-reactive studies with human tissue typing sera. Br J Exp Path 1980; 61: 92-6.

  6. Collado A, Gratacos J, Ebringer A, Rashid T, Marti A, Sammarti R, Munoz-Gomez J. Serum IgA anti-Klebsiella pneumoniae in ankylosing spondylitis patients from Catalonia. Scand J Rheumatol 1994; 23: 119-23.

  7. Hermann E, Sukke B, Droste U, Büschenfelde KM. Klebsiella pneumoniae reactive T cells in blood and synovial fluid of patients with ankylosing spondylitis. Arthritis Rheum 1995; 38: 1277-82.

  8. Ebringer RW, Cawdell DR, Cowling P, Ebringer A. Sequential studies in ankylosing spondylitis: association of Klebsiella pneumoniae with active disease. Ann Rheum Dis 1978; 37: 146-51.

  9. Welsh J, Avakian H, Cowling P, Ebringer A, Wooley P, Panayi G, Ebringer R. Ankylosing spondylitis, HLA-B27 and Klebsiella pneumoniae I: cross-reactive studies with rabbit antisera. Br J Exp Path 1980; 61: 85-91.

  10. Parra-Campos V, Escobar-Gutiérrez A, Cancino-Díaz M, Domínguez-López ML, Burgos-Vargas R, Granados-Arreola J, García-Latorre E. Antibody response to nitrogenase-positive and-negative Klebsiella pneumoniae strains in juvenile-onset ankylosing spondylitis patients and their first degree relatives: lack of differential recognition of the bacterial nitrogenase. Rev Lat-amer Microbiol 1996; 38: 121-7.

  11. Cancino-Díaz ME, Pérez-Salazar JE, Domínguez-López ML. Escobar-Gutiérrez A, Granados-Arreola J, Jiménez-Zamudio L, Burgos-Vargas R, García-Latorre E. Antibody response to Klebsiella pneumoniae 60 kDa protein in familial and sporadic ankylosing spondylitis: role of HLA-B27 and protein characterization as a Gro-EL HSP60. J Rheumatol 1998; 25: 1756-64.

  12. Cancino-Díaz M, Curiel-Quesada E, García-Latorre E, Jiménez-Zamudio L. Cloning and sequencing of the gene that codes for the HSP60 of Klebsiella pneumoniae, related to ankylosing spondylitis. Microb Path 1998; 25: 23-32

  13. Domínguez-López ML, Cancino-Díaz ME, Jiménez-Zamudio L, Granados-Arreola J, Burgos-Vargas R, García-Latorre E. Cellular immune response to Klebsiella pneumoniae antigens in HLA-B27 positive ankylosing spondylitis patients. J Rheumatol 2000; 27: 1453-60.

  14. Domínguez-López ML, Burgos-Vargas R, Galicia-Serrano H, Bonilla-Sánchez MT, Rangel-Acosta HH, Cancino-Díaz ME, Jiménez-Zamudio L, Granados J, García-Latorre E. IgG antibodies to enterobacteria 60 kDa heat shock proteins in the sera of HLA-B27 positive ankylosing spondylitis patients. Scand J Rheumatol 2002; 31: 260-5.

  15. Fiorillo MT, Maragna M, Butler R, Dupuis ML, Sorrentino R. CD8+ T-cell autoreactivity to an HLA-B27-restricted self-epitope corrrelates with Ankylosing spondylitis. J Clin Invest 2000; 106: 47-53.

  16. Schirmer M, Golberger C, Würzner R, Duftner C, Pfeiffer KP, Clausen J, Naumayr G, Falkenbach A. Circulating cytotoxic CD8+CD28- T cells in ankylosing spondylitis. Arthritis Res 2002; 4: 71-6.

  17. Lehner PJ, Cresswell P. Processing and delivery of peptides presented by MHC class I molecules. Curr Opin Immunol 1996; 8: 59-67.

  18. Pamer EG. Antigen presentation in the immune response to infectious diseases. Clin Infect Dis 1999; 28: 714-6.

  19. Carbonetti NH, Irish TJ, Chen CH, O’Connell CB, Hadley GA, McNamara U, Tuskan, RG, Lewis GK. Intracellular delivery of a cytolytic T-lymphocyte epitope peptide by pertussis toxin to major histocompatibility complex class I without involvement of the cytosolic class I antigen processing pathway. Infect Immun 1999; 67: 602-7.

  20. Health WR, Carbone FR. Cross-presentation in viral immunity and self tolerance. Nature Rev 2001; 1: 127-34.

  21. Jensen PE, Weber DA, Thayer WP, Westerman LE, Dao CT. Peptide exchange in MHC molecules. Immunol Rev 1999; 172: 229-38.

  22. Reimann J, Kaufmann HE. Alternative antigen processing pathways in antiinfective immunity. Curr Op Immunol 1997; 9: 462-9.

  23. Reimann J, Schirmbeck R. Alternative pathways for processing exogenous antigens that can generate peptides for MHC class I-restricted presentation. Immunol Rev 1999; 172: 131-52.

  24. Wick MJ, Ljunggren HG. Processing of bacterial antigens for peptide presentation on MHC class I molecules. Immunol Rev 1999; 172: 153-62.

  25. Zhou X, Franksson LL, Lederer E, Ljunggren HG, Jondal M. Characterization of TAP-independent and brefeldin A-resistant presentation of sendai virus antigen to CD8+ cytotoxic T lymphocytes. Scand J Immunol 1995; 42: 66-75.

  26. Kovacsovics-Bankowski M, Rock KL. A phagosome-to-cytosol pathway for exogenous antigens presented on MHC class I molecules. Science 1995; 267: 243-6.

  27. Bennet PH, Burch TA. New York Symposium on population studies in the rheumatic diseases: new diagnostic criteria. Bull Rheum Dis 1967; 17: 453-8.

  28. Bradford M. A rapid and sensitive method for the quantitation of micrograms quantities of proteins utilizing the principle of protein dye binding. Anal Biochem 1976; 72: 248.

  29. Braine HG, Elfenbeing GJ, Mellits ED. Peripheral blood lymphocyte numbers, lymphocyte proliferative responses in vitro, and serum immunoglobulins in regular hemapheresis donors. J Clin Apheresis 1985; 2: 213-8.

  30. Donalson SL, Ranney RR, Tew JG. Evidence of mitogenic activity in periodontitis-associated bacteria. Infect Immun 1983; 42: 487-95.

  31. Feurle GE, Dörken B, Schöpf E, Lenhard V. HLA-B27 and defects in the T cell system in Whipple’s disease. Eur J Clin Invest 1979; 9: 385-9.

  32. Men Y, Audran R, Thomasin C, Eberl G, Demotz S, Merkle HP, Gander B Corradin G. MHC class I- and class II-restricted processing and presentation of microencapsulated antigens. Vaccine 1999; 17: 1047-56.

  33. Afeltra A, Galeazzi M, Ferri G, Amoroso A, De Pita O, Porzio F, Bonomo L. Expression of CD69 antigen on synovial fluid T cells in patients with rheumatoid arthritis and other chronic synovitis. Ann Rheum Dis 1993; 52: 457-60.

  34. Hernandez-García C, Fernández-Gutiérrez B, Morado C, Bañares A, Jover J. The CD69 activation pathway in rheumatoid arthritis synovial fluid T cells. Arthritis Rheum 1996; 39: 1277-86.

  35. Stone MA, Payne U, Schentag C, Rahman P, Pacheco-Tena C, Inman RD. Comparative immune responses to candidate arthritogenic bacteria do not confirm a dominant role for Klebsiella pneumoniae in the pathogenesis of familial ankylosing spondylitis. Rheumatol 2004; 43: 148-55.

  36. Parga Lozano C, Marrugo Cano J, Hernández Bonfante L. Respuesta proliferativa de células mononucleares de sangre periférica frente al alérgeno recombinante BtM del ácaro del polvo casero Blomia tropicalis. Allergol et Immunopathol 2004; 32: 247-51.

  37. Hutchinson P, Divola LA, Holdsworth R. Mitogen-induced T-cell CD69 expression is a less sensitive measure of T cell function than [3H]-thymidine uptake. Cytometry 1999; 38: 244-9.

  38. Lich JD, Elliot JF, Blum JS. Cytoplasmic processing is a prerequisite for presentation of endogenous antigen by major histocompatibility complex class II proteins. J Exp Med 2000; 191: 1513-23.

  39. Rutella S, Rumi C, Lucia MB, Barberi T, Puggioni PL, Lai M, Romano A, Cauda R, Leone G. Induction of CD69 antigen on normal CD4+ and CD8+ lymphocyte subsets and its relation-ship with phenotype of responding T cells. Cytometry 1999; 38: 95-101.

  40. Augustin B, Kotnik V, Skoberne M, Malovrh T, Skralovnick-Stern A, Tercelj M. CD 69 expression on CD4+ T lymphocyte after in vitro stimulation with tuberculin is an indicator of immune sensitization against Mycobacterium tuberculosis antigens. Clin Diagn Lab Immunol 2005; 12: 101-6.




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