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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2005, Número 4

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Rev Invest Clin 2005; 57 (4)


Respuesta de linfocitos T CD4 y CD8 contra la rHSP60 de Klebsiella pneumoniae en células mononucleares de sangre periférica de pacientes con espondilitis anquilosante

Zambrano-Zaragoza F, García-Latorre E, Domínguez-López ML, Cancino-Díaz ME, Burgos-Vargas R, Jiménez-Zamudio L

Idioma: Ingles.
Referencias bibliográficas: 40
Paginas: 555-562
Archivo PDF: 294.62 Kb.


RESUMEN

Objetivo. Determinar las vías utilizadas por las células mononucleares de sangre periférica (CMSP) de pacientes con espondilitis anquilosante para procesar a la rHSP60 de Klebsiella pneumoniae (rHSP60Kp) y las subpoblaciones de linfocitos T involucrados en la activación. Métodos. Se determinó la res-puesta linfoproliferativa, por incorporación de 3H-TdR en CMSP, en presencia de la rHSP60Kp, en 14 pacientes con EA HLA-B27+ y en 15 sujetos sanos HLA-B27-. La ruta de procesamiento y presentación de la rHSP60Kp se determinó por incorporación de 3H-TdR en las CMSP de los pacientes utilizando como células presentadoras a las CMSP homólogas, preincubadas con el antígeno y los inhibidores específicos: cloroquina, brefeldina A y N-acetil-L-leucil-L-leucil-L-nor-leucinal (LLnL), y fijadas con p-formaldehído. Se evaluaron las subpoblaciones de linfocitos T CD4+ y CD8+ que expresaron CD69, frente al antígeno, por citometría de flujo. Resultados. Ocho de los 14 pacientes y ninguno de los sujetos sanos, tuvo respuesta linfoproliferativa positiva (IE › 3.0) contra la rHSP60Kp (p ‹ 0.012). En cinco de los pacientes el I.E. fue superior a 4.0. En estos pacientes la linfoproliferación disminuyó cuando se utilizó cloroquina y LLnL, y se hizo negativa cuando se utilizó BFA, lo que indica que ambas vías son empleadas. Las subpoblaciones de linfocitos T (CD4+ y CD8+) expresaron CD69 frente al antígeno. Conclusiones. Nuestros resultados sugieren que ambas poblaciones de linfocitos T: CD4+ y CD8+ participan en la respuesta a la rHSP60Kp.


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