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ISSN 2007-4360 (Print)
Asociación Mexicana de Periodontología, Colegio de Periodoncistas A.C

2015, Number 3

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Rev Mex Periodontol 2015; 6 (3)

Decrease in the nuclear and oxidative DNA damage in patients with periodontitis after intake of folic acid

Gómez MJC, Morales VG, Ortiz GYM, Gómez MBC, Guerreo VC, Mendoza LAL, Martínez RVMC, Zamora PAL

Language: Spanish
References: 20
Page: 121-128
PDF size: 323.70 Kb.


ABSTRACT

Introduction: Periodontitis (PE) is a disease that not only affects the oral cavity, but also disturbs the overall health of the individual since the PE course with an increased in the production of oxidative stress and free radicals (FR) which increases DNA damage. Antioxidants, such as folic acid (FA), can act as an adjuvant to strengthen the antioxidant defense system and prevent the damage that causes the FR in PE. Objective: Determine the nuclear and oxidative DNA damage in patients with PE after (FA) intake. Material and methods: Two groups were formed: group 1 (n = 30) subjects without PE and group 2 (n = 35): subjects with PE. Both groups were exposed to 5 mg of FA orally 3 times daily for 30 days. From all participants, buccal mucosa and saliva samples were taken, one at the beginning of FA intake and the next 30 days later. Nuclear damage was determined by means of micronuclei (MN) and nuclear abnormalities (NA) assay in buccal mucosa cells. Oxidative damage was determined by quantifying the levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) in saliva by ELISA assay. Results: The PE group presented significant increase numbers (p ‹ 0.05) in MN, NA and in the levels of 8-OHdG in the baseline sample compared with the group without PE. And after the intake of FA the values of MN, NA and 8-OHdG decreased statistically significant (p ‹ 0.05) when compared with baseline values, in both groups. Conclusion: The number of MN, NA and 8-OHdG levels was significantly decreased in patients with PE and in the group without PE after folic acid intake.


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