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2002, Number 4

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Rev Inst Nal Enf Resp Mex 2002; 15 (4)

Preliminary characterization of the protein composition of buildings located in different parts of the México City metropolitan area.

Haselbarth LMMM, Pérez NJ, Arreguín ER

Language: Spanish
References: 50
Page: 206-213
PDF size: 84.43 Kb.


ABSTRACT

Introduction: In children, asthma is among the most important respiratory health problems. It has been recognized that one of the main risk factors for the development of asthmatic symptoms is exposure to indoors allergens, especially from house dust. Although the prevalence of asthma in Mexico lies between 5 to 10 %, published reports on the protein composition of house dust are scarce. The objective of our study was a qualitative and quantitative characterization of the protein composition of some samples of house dust obtained from different areas of Mexico City and to look for a correlation with some house features, such as humidity, pets or carpets. Material and methods: Thirty-one samples of dust obtained from houses randomly selected from a group of children in Mexico City public schools were analyzed. Dust proteins were characterized by a colorimetric method and quantitatively analyzed by electrophoresis in a sodium dodecil sulfate polyacrylamide gel (SDS-PAGE). Results: Proteins constituted a mean of 0.2% of each house dust sample with concentrations that varied from 0.036 to 9.030 mg/g of dust. The Mann-Whitney test showed no correlation between protein concentration and home characteristics. The most frequent protein molecular weights ranged between 13.6 to 16.5 kDa. Other molecular weights present were 10.6 to 13.5, 34.6 to 37.5, 40.6 to 43.5 and 58.6 to 61.5 kDa. The presence of these proteins did not depend on any of the house features; however, the proteins with molecular weight between 40.6 to 43.5 kDa showed a significant statistical correlation (p‹ 0.05) with the presence of carpets in the house (Fisher exact test). Also, this range of molecular weights had a probability 6.86 higher (C.I. 1.10, 42.76) in houses with carpets than in houses without them (OR). To the best of our knowledge, this is the first study performed in Mexico aimed to characterize proteins in house dust, and present results can serve as a basis for future studies on biologic indoor pollution.


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