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2015, Number 3

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Evid Med Invest Salud 2015; 8 (3)

Cytotoxic effect of the Vibrio cholerae cytolysin (VCC) on HEK293 cells

Castañón-Sánchez CA, Enríquez-Rincón F, Figueroa-Arredondo P

Language: Spanish
References: 20
Page: 111-116
PDF size: 359.34 Kb.


ABSTRACT

The non-O1 Vibrio cholerae group is composed by many diverse strains that are non-cholera toxin producers (CT -) or non-toxigenic strains), but still they are diarrheagenic and cytotoxic. These characteristics are attributed to the presence of a pore-forming toxin: the V. cholerae cytolysin (also known as VCC), since it is the best candidate to cause diarrhea in the human host. Characterization of the VCC as a vacuolation-inducer toxin was previously conducted by our group in tumorigenic and immortalized cell lines. In order to continue the study of the cytotoxicity of the VCC, we have chosen the HEK-293 cell line as a model of study that would help to further determine the role of the VCC as a virulence factor in the non O1 V. cholerae pathogenesis, since these cells are used for foreign protein expression from plasmids with the cloned genes. In vitro challenges were performed applying sterile bacterial supernatants rich in VCC (1:20) to HEK-293 cells, and later on, a microscopic search for cytotoxicity was performed. Here we show that there is a cytotoxic effect on HEK-293 cells, directly related to VCC, the toxin hyper-produced by V. cholerae non-O1 strains from our strain collection.


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