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TIP Revista Especializada en Ciencias Químico-Biológicas

2015, Number 2

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TIP Rev Esp Cienc Quim Biol 2015; 18 (2)

Male infertility and spermatic DNA fragmentation: A current problem

Quintero–Vásquez GA, Bermúdez-Cruz RM, Castillo-Cadena J

Language: Spanish
References: 87
Page: 144-151
PDF size: 741.38 Kb.


ABSTRACT

Spermatic DNA contributes one half of the genetic material to offspring. Currently, the parameters obtained through a seminogram do not provide complete information on the potential fertilization of the semen and the ability to produce a healthy embryo and an on-going pregnancy. This is the reason why the interest in developing techniques to assess sperm DNA fragmentation has been increased, as damage during spermatogenesis can occur at any stage of the process, this being a multifactorial phenomenon and not entirely delimited yet. Nowadays the infertility is an increasing global problem, and it has been shown that the quality of the DNA in the sperm can affect fertilization; this is why the evaluation of sperm DNA integrity, in addition to the study of the sperm parameters may provide additional information on the quality of the spermatozoa, resulting in great help to identify the causes of male infertility and thus can be used to better guide couples with infertility issues.


REFERENCES

  1. WHO Biomarkers and Risk Assessment: Concepts and Principle. World Health Organization (1990).

  2. Nallella, K.P., Sharma, R.K., Aziz, N. & Agarwal, A. Significance of sperm characteristics in the evaluation of male infertility. Fertil Steril 85, 629-634 (2006).

  3. Spira, A. & Multigner, L. Sperm density and environment. Treatment of infertility: the new frotiers (1998).

  4. Alshahrani, S. et al. Infertile men older than 40 years are at higher risk of sperm DNA damage. Reprod. Biol. Endocrinol. 12, 103 (2014).

  5. Enciso, M., Alfarawati, S. & Wells, D. Increased numbers of DNAdamaged spermatozoa in samples presenting an elevated rate of numerical chromosome abnormalities. Hum. Reprod. 28, 1707-1715 (2013).

  6. Gallegos, G. et al. Sperm DNA fragmentation in infertile men with genitourinary infection by Chlamydia trachomatis and Mycoplasma. Fertil Steril 90, 328-334 (2008).

  7. Li, W.W. et al. Cigarette smoking affects sperm plasma membrane integrity. Zhonghua Nan Ke Xue 18, 1093-1096 (2013).

  8. Martini, A.C. et al. Effects of alcohol and cigarette consumption on human seminal quality. Fertil Steril 82, 374-377 (2004).

  9. Sánchez-Peña, L.C. et al. Organophosphorous pesticide exposure alters sperm chromatin structure in Mexican agricultural workers. Toxicol. Appl. Pharmacol. 196, 108-113 (2004).

  10. Schmid, T.E. et al. Elemental composition of human semen is associated with motility and genomic sperm defects among older men. Hum. Reprod. 28, 274-282 (2013).

  11. Menkveld, R. The basic semen analysis. Chapter 9. In: Male Infertility. Diagnosis and treatment (eds. Oehninger, S. & Kruger, T.F.) 141-170 (Oxon, UK, Informa Healthcare, 2007).

  12. McLachlan, R.I. et al. Semen analysis: its place in modern reproductive medical practice. Pathology 35, 25-33 (2003).

  13. WHO Laboratory Manual for the examination and Processing of Human Semen. World Health Organization press 5th edn (2010).

  14. Comhaire, F. & Vermeulen, L. Human semen analysis. Hum Reprod Update 1, 343-362 (1995).

  15. Cocuzza, M. Clinical relevance of routine semen analysis and controversies surrounding the 2010 World Health Organization criteria for semen examination. Int. Braz. J. Urol. 41, 181-183 (2010).

  16. Barratt, C.L., Bjorndahl, L., Menkveld, R. & Mortimer, D. Special interest group for andrology basic semen analysis course: a continued focus on accuracy, quality, efficiency and clinical relevance. Hum. Reprod. 26, 3207-3212 (2011).

  17. Guzick, D.S. et al. Efficacy of treatment for unexplained infertility. Fertil. Steril. 70, 207-213 (1998).

  18. Carrell, D.T. et al. Sperm DNA fragmentation is increased in couples with unexplained recurrent pregnancy loss. Arch. Androl. 49, 49-55 (2003).

  19. Khadem, N., Poorhoseyni, A., Jalali, M., Akbary, A. & Heydari, S.T. Sperm DNA fragmentation in couples with unexplained recurrent spontaneous abortions. Andrologia 46, 126-130 (2014).

  20. Sedlackova, T., Repiska, G., Celec, P., Szemes, T. & Minarik, G. Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods. Biol. Proced. Online 15, 5 (2013).

  21. Irvine, D.S. et al. DNA integrity in human spermatozoa: relationships with semen quality. J. Androl. 21, 33-44 (2000).

  22. De Kretser, D.M., Loveland, K.L., Meinhardt, A., Simorangkir, D. & Wreford, N. Spermatogenesis. Hum. Reprod. 13 Suppl 1, 1-8 (1998).

  23. Rajender, S., Avery, K. & Agarwal, A. Epigenetics, spermatogenesis and male infertility. Mutat. Res. 727, 62-71 (2011).

  24. Tesarik, J., Guido, M., Mendoza, C. & Greco, E. Human spermatogenesis in vitro: respective effects of follicle-stimulating hormone and testosterone on meiosis, spermiogenesis, and Sertoli cell apoptosis. J. Clin. Endocrinol. Metab. 83, 4467- 4473 (1998).

  25. Aoki, V.W. et al. DNA integrity is compromised in protaminedeficient human sperm. J. Androl. 26, 741-748 (2005).

  26. Balhorn, R. The protamine family of sperm nuclear proteins. Genome Biol. 8, 227 (2007).

  27. Carrell, D.T., Emery, B.R. & Hammoud, S. Altered protamine expression and diminished spermatogenesis: what is the link? Hum. Reprod. Update 13, 313-327 (2007).

  28. Iranpour, F.G. The effects of protamine deficiency on ultrastructure of human sperm nucleus. Adv. Biomed. Res. 3, 24 (2014).

  29. Jodar, M. & Oliva, R. Protamine alterations in human spermatozoa. Adv. Exp. Med. Biol. 791, 83-102 (2013).

  30. Liu, L., Aston, K.I. & Carrell, D.T. Protamine extraction and analysis of human sperm protamine 1/protamine 2 ratio using Acid gel electrophoresis. Methods Mol. Biol. 927, 445-450.

  31. Hekmatdoost, A., Lakpour, N. & Sadeghi, M.R. Sperm chromatin integrity: etiologies and mechanisms of abnormality, assays, clinical importance, preventing and repairing damage. Avicenna J. Med. Biotechnol. 1, 147-160 (2009).

  32. Agarwal, A. & Said, T.M. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum. Reprod. Update 9, 331-345 (2003).

  33. Yu, B. et al. Cigarette smoking is associated with abnormal histone-to-protamine transition in human sperm. Fertil Steril 101, 51-57 (2013).

  34. Agarwal, A. & Saleh, R.A. Utility of oxidative stress test in the male infertility clinic Zhonghua Nan Ke Xue 8, 1-9 (2002).

  35. Oliva A, S.A. & Multigner, L. Contribution of enviromental factors to the risk of male infertility. Hum. Reprod. 16 1768-1776 (2011).

  36. Godmann, M., Lambrot, R. & Kimmins, S. The dynamic epigenetic program in male germ cells: Its role in spermatogenesis, testis cancer, and its response to the environment. Microsc. Res. Tech. 72, 603-619 (2009).

  37. Agarwal, S., Sikka, M., Dev, G. & Banerjee, A. Testicular size, morphology and spermatogenesis in infertile males with varicocele. Indian J. Pathol. Microbiol. 31, 19-25 (1988).

  38. Wright, C., Milne, S. & Leeson, H. Sperm DNA damage caused by oxidative stress: modifiable clinical, lifestyle and nutritional factors in male infertility. Reprod. Biomed. Online 28, 684-703 (2014).

  39. Aitken, R.J. & Krausz, C. Oxidative stress, DNA damage and the Y chromosome. Reproduction 122, 497-506 (2001).

  40. Agarwal, A. & Allamaneni, S.S. Sperm DNA damage assessment: a test whose time has come. Fertil Steril 84, 850-853 (2005).

  41. Koskimies, A.I., Savander, M., Ann-Marie, N. & Kurunmaki, H. Sperm DNA damage and male infertility. Duodecim. 126, 2837-2842 (2011).

  42. Zini, A., Bielecki, R., Phang, D. & Zenzes, M.T. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril 75, 674-677 (2001).

  43. Zhang, X.D. et al. The effects of different sperm preparation methods and incubation time on the sperm DNA fragmentation. Hum. Fertil (Camb.) 14, 187-191 (2011).

  44. Martínez-Pastor, F., Fernández-Santos, M.R., Domínguez- Rebolledo, A.E., Esteso, M.C. & Garde, J.J. DNA status on thawed semen from fighting bull: a comparison between the SCD and the SCSA tests. Reprod. Domest. Anim. 44, 424-431 (2009).

  45. Ribas-Maynou, J. et al. Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay. Andrology 1, 715-722 (2013).

  46. Durmaz, A. et al. COMET, TUNEL, and TEM analysis of an infertile male with short tail sperm. J. Turk. Ger. Gynecol. Assoc. 16, 54-57 (2015).

  47. Chenlo, P.H. et al. Fragmentation of sperm DNA using the TUNEL method. Actas Urol. Esp. 38, 608-612 (2014).

  48. Curi, S.M. et al. Flow cytometry TUNEL standardization for assaying sperm DNA fragmentation. J. Androl. 33, 1 (2011).

  49. Domínguez-Fandos, D., Camejo, M.I., Ballesca, J.L. & Oliva, R. Human sperm DNA fragmentation: correlation of TUNEL results as assessed by flow cytometry and optical microscopy. Cytometry A. 71, 1011-1018 (2007).

  50. Chesselet, M.F., MacKenzie, L. & Hoang, T. Detection of DNA damage in tissue sections by in situ nick translation. Curr. Protoc. Neurosci. Chapter 1, Unit 19 (2001).

  51. Gorczyca, W., Gong, J. & Darzynkiewicz, Z. Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res. 53, 1945-1951 (1993).

  52. Aravindan, G.R., Bjordahl, J., Jost, L.K. & Evenson, D.P. Susceptibility of human sperm to in situ DNA denaturation is strongly correlated with DNA strand breaks identified by single-cell electrophoresis. Exp. Cell Res. 236, 231-237 (1997).

  53. Fairbairn, D.W., Olive, P.L. & O’Neill, K.L. The comet assay: a comprehensive review. Mutat. Res. 339, 37-59 (1995).

  54. Bungum, M., Bungum, L. & Giwercman, A. Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility. Asian J. Androl. 13, 69-75 (2011).

  55. Evenson, D.P. Sperm chromatin structure assay (SCSA). Methods Mol. Biol. 927, 147-164 (2012).

  56. Evenson, D.P. & Wixon, R. Comparison of the Halosperm test kit with the sperm chromatin structure assay (SCSA) infertility test in relation to patient diagnosis and prognosis. Fertil Steril 84, 846-849 (2005).

  57. Henkel, R., Hoogendijk, C.F., Bouic, P.J. & Kruger, T.F. TUNEL assay and SCSA determine different aspects of sperm DNA damage. Andrologia 42, 305-313 (2010).

  58. Fernández, J.L. et al. Simple determination of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil Steril 84, 833-842 (2005).

  59. Fernández, J.L. et al. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentation. J. Androl. 24, 59-66 (2003).

  60. Muriel, L. et al. Increased aneuploidy rate in sperm with fragmented DNA as determined by the sperm chromatin dispersion (SCD) test and FISH analysis. J. Androl. 28, 38-49 (2007).

  61. Ahmadi, A. & Ng, S.C. Fertilizing ability of DNA-damaged spermatozoa. J. Exp. Zool. 284, 696-704 (1999).

  62. Ahmadi, A. & Ng, S.C. Developmental capacity of damaged spermatozoa. Hum. Reprod. 14, 2279-2285 (1999).

  63. Spano, M., Seli, E., Bizzaro, D., Manicardi, G.C. & Sakkas, D. The significance of sperm nuclear DNA strand breaks on reproductive outcome. Curr. Opin. Obstet. Gynecol. 17, 255-260 (2005).

  64. Wossidlo, M. et al. Dynamic link of DNA demethylation, DNA strand breaks and repair in mouse zygotes. EMBO. J. 29, 1877-1888 (2010).

  65. Enciso, M. et al. The ability of sperm selection techniques to remove single- or double-strand DNA damage. Asian J. Androl. 13, 764-768 (2011).

  66. Derijck, A., van der Heijden, G., Giele, M., Philippens, M. & de Boer, P. DNA double-strand break repair in parental chromatin of mouse zygotes, the first cell cycle as an origin of de novo mutation. Hum. Mol. Genet. 17, 1922-1937 (2008).

  67. Daris, B., Goropevsek, A., Hojnik, N. & Vlaisavljevic, V. Sperm morphological abnormalities as indicators of DNA fragmentation and fertilization in ICSI. Arch. Gynecol. Obstet. 281, 363-367 (2009).

  68. Frydman, N. et al. Adequate ovarian follicular status does not prevent the decrease in pregnancy rates associated with high sperm DNA fragmentation. Fertil Steril 89, 92-97 (2008).

  69. Kennedy, C., Ahlering, P., Rodríguez, H., Levy, S. & Sutovsky, P. Sperm chromatin structure correlates with spontaneous abortion and multiple pregnancy rates in assisted reproduction. Reprod. Biomed. Online 22, 272-276 (2011).

  70. Lin, M.H. et al. Sperm chromatin structure assay parameters are not related to fertilization rates, embryo quality, and pregnancy rates in in vitro fertilization and intracytoplasmic sperm injection, but might be related to spontaneous abortion rates. Fertil Steril 90, 352-359 (2008).

  71. Simon, L., Lutton, D., McManus, J. & Lewis, S.E. Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success. Fertil Steril 95, 652-657 (2010).

  72. Lewis, S.E., O’Connell, M., Stevenson, M., Thompson-Cree, L. & McClure, N. An algorithm to predict pregnancy in assisted reproduction. Hum. Reprod. 19, 1385-1394 (2004).

  73. Payne, J.F. et al. Redefining the relationship between sperm deoxyribonucleic acid fragmentation as measured by the sperm chromatin structure assay and outcomes of assisted reproductive techniques. Fertil Steril 84, 356-364 (2005).

  74. Fujimoto, V.Y., Browne, R.W., Bloom, M.S., Sakkas, D. & Alikani, M. Pathogenesis, developmental consequences, and clinical correlations of human embryo fragmentation. Fertil Steril 95, 1197-1204.

  75. Stigliani, S., Anserini, P., Venturini, P.L. & Scaruffi, P. Mitochondrial DNA content in embryo culture medium is significantly associated with human embryo fragmentation. Hum. Reprod. 28, 2652-2660 (2013).

  76. Pregl Breznik, B., Kovacic, B. & Vlaisavljevic, V. Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization and intracytoplasmic sperm injection? Fertil Steril 99, 1233-1241 (2013).

  77. Sakkas, D. et al. Origin of DNA damage in ejaculated human spermatozoa. Rev. Reprod. 4, 31-37 (1999).

  78. Muratori, M. et al. Investigation on the Origin of Sperm DNA Fragmentation: Role of Apoptosis, Immaturity and Oxidative Stress. Mol. Med. (2015).

  79. Pan, F. et al. Seminal parameters at different times of reanalysis of the normal semen samples detected in initial examination and their correlation with sperm DNA damage. Zhonghua Nan Ke Xue 19, 63-67 (2013).

  80. Shaman, J.A., Yamauchi, Y. & Ward, W.S. Sperm DNA fragmentation: awakening the sleeping genome. Biochem. Soc. Trans. 35, 626-628 (2007).

  81. Lewis, S.E., Agbaje, I. & Álvarez, J. Sperm DNA tests as useful adjuncts to semen analysis. Syst. Biol. Reprod. Med. 54, 111- 125 (2008).

  82. Lopes, S., Sun, J.G., Jurisicova, A., Meriano, J. & Casper, R.F. Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril 69, 528-532 (1998).

  83. Sun, J.G., Jurisicova, A. & Casper, R.F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol. Reprod. 56, 602- 607 (1997).

  84. Huang, C.C. et al. Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertil Steril 84, 130-140 (2005).

  85. Drobnis, E.Z. & Johnson, M.H. Are we ready to incorporate sperm DNA-fragmentation testing into our male infertility work-up? A plea for more robust studies. Reprod. Biomed. 30, 111-112 (2015).

  86. Evgeni, E., Charalabopoulos, K. & Asimakopoulos, B. Human sperm DNA fragmentation and its correlation with conventional semen parameters. J. Reprod. Infertil 15, 2-14 (2014).

  87. Zini, A. Are sperm chromatin and DNA defects relevant in the clinic? Syst. Biol. Reprod. Med. 57, 78-85 (2011).




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