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2000, Number 4

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Rev Inst Nal Enf Resp Mex 2000; 13 (4)

Effects of cumarine and 7-hydroxicumarine on the cell cycle of pulmonary adenocarcinoma cell lines.

Sullivan López-González J, Prado GH, Aguilar CD, Molina GJA, Mandoki JJ, Medina MF

Language: Spanish
References: 26
Page: 192-197
PDF size: 55.69 Kb.


ABSTRACT

Introduction: Coumarins are compounds widely distributed in nature. In humans, the main metabolite of coumarin is 7-hydroxicoumarin. These compounds show antitumoral activity in vivo and in vitro. We previously reported that coumarin and 7-hydroxicoumarin have cytostatic activity in adenocarcinoma cell lines; however, no reports are available concerning the cell cycle phase in which these compounds may act. Objective: To determine the effect of coumarin and 7-hydroxicoumarin on the cell cycle transition in lung adenocarcinoma cell lines. Material and methods. Three adenocarcinoma cell lines (SK-LU-1, 1.3.15, 3A5A) and healthy donor mononuclear cells stimulated with phytohemagglutinin were incubated for 24h and 40h respectively with 100 mg/mL of coumarin or 7-hydroxicoumarin. Cell cycle disturbances were evaluated by flow cytometry using DNA content analysis. Results: After treatment, the cell lines showed statistically significant accumulation of the G0/GI cell cycle phase, accompanied by a proportional depletion of the S-phase. Nevertheless, no significant changes were observed in mononuclear cells after treatment. Conclusions: Results show that coumarin and 7-hydroxicoumarin arrest tumoral cells at the G0/GI phase. Understanding the cell cycle disturbances induced by these compounds will help in the design of treatment schedules by combination with other drugs that arrest the cell cycle at other phases. Furthermore, this methodology can be used to test tumor cell susceptibility against chemotherapeutic agents.


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