2002, Number 2
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Rev Mex Patol Clin Med Lab 2002; 49 (2)
Evaluation of the efficacy diagnosis of ADN waves fresh examination, in patients with vaginal infection
Montes OAE, García ERM, Bernal GMJ, Pérez RC
Language: Spanish
References: 18
Page: 100-107
PDF size: 78.63 Kb.
ABSTRACT
In order to find out the difference in efficacy for mycrobiologic agent analysis of patients, suffering of vaginal infection: ADN waves versus microbiologic analysis of vaginal fresh material based on Nuget criteria were assayed. Sixty five patients who attended to the laboratory for analysis of the exudates from the vagina fundus were included in this study. Exudate sample from the vagina was taken with no lubricant throughout cotton or dracon swab. The smear of the exudates were Gram stainned to microscopically identify:
Candida, Thrichomonas, Gardnerella and clue cells. Samples were stripped in Agar Casman. Once the colonies had appeared, those which resulted suspicious for Candida were restripped in Nickerson media. For pathogenic Candida differentiation, a filamentous serum test was assayed. Findings of this search resulted as follows: Nine samples had
Candida (13.8%); 4 (6.1%) were pathogenic and 5 (6.5%) were not. One sample had Trichomonas (1.5%). Twenty six had Gardnerella (40%) and twenty nine were negative for mycrobiologic identification. Analysis with the DNA waves technique resulted as follows:
Trichomonas vaginalis 1 (1.5%),
Gardnerella vaginalis 26 (40.0%) and 38 were negative (58.5%)
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