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2002, Number 2

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Vet Mex 2002; 33 (2)

Comparative study of the concentration of reduced glutathione and the activity of gamma-glutamyl-transferase and glutathione transferase in liver and kidney of broilers and rats

Quezada TT, Jaramillo JF, Valdivia FA, Reyes SJL, Ortiz MR, Rodríguez VML

Language: English/Spanish
References: 43
Page: 125-135
PDF size: 137.28 Kb.


ABSTRACT

The objective of this study was to compare protein concentration (PC), adenosine 5'-triphosphate (ATP) and reduced glutathion (GSH), as well as the specific enzymatic activities of transferase of glutathion (SEA-TG), and gamma-glutamyl-transferase (SEA-GGT) in liver and kidney homogenates of broilers and rats. Due to the fact that these processes are involved in the detoxification process of the cells, twenty-five four-week-old broilers, and twenty-five 35 day-old rats were divided at random in five groups. Animals were weighed, anesthetized, and perfused using chicken buffer solution, and their liver and kidneys were removed. Samples from both tissues were taken and homogenized in a buffer solution (4°C). Aliquots were made and stored at –25°C until analyzed. Protein concentration (mg/g of tissue), ATP (ng/mg of tissue), and GSH (Mol/mg of tissue) were determined. AEE-TG and AEE-GGT were measured. Data was analyzed by the analysis of variance (ANOVA), and the multiple comparison means was obtained through the Tukey test. ATP and GSH, as well as SEA-GGT-and SEA-TG concentrations were significant among species and tissues (P ‹ 0.01). PC among species in both tissues did not show significant differences (P › 0.05), while that among the tissues of the same species proved to be different (P ‹ 0.01). Results of the comparison of the studied species suggest, in general, discrepancy between species, and the possible difference of response of cellular detoxification mechanisms in presence of xenobiotics.


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