medigraphic.com
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2013, Number 6

<< Back Next >>

Rev Invest Clin 2013; 65 (6)

Safety of a dual potential prebiotic system from Mexican agave “Metlin® and Metlos®”, incorporated to an infant formula for term newborn babies: a randomized controlled trial

López-Velázquez G, Díaz-García L, Anzo A, Parra-Ortiz M, Llamosas-Gallardo B, Ortiz-Hernández AA, Mancilla-Ramírez J, Cruz-Rubio JM, Gutiérrez-Castrellón P

Language: English
References: 40
Page: 483-490
PDF size: 166.17 Kb.


ABSTRACT

Rationale. Infant formulae are being supplemented with probiotics, prebiotics, or symbiotic despite uncertainties regarding their efficacy. Mexican agave is an interesting source of fructans with particular features and with potential prebiotic effects. Material and methods. RCT in 600 healthy term babies (20 ± 7 days), allocated to receive standard infant formula (control) or infant formula added with a dual prebiotic system “Metlin® and Metlos®”, from Mexican agave. Primary outcomes include stools frequency, stools consistency, gastrointestinal intolerance (frequency of abdominal distension, flatulency, regurgitations, vomiting). Secondary outcomes include changes on weight and height along the study and frequency of dermatologic problems (eczema). Results. In 66,120 days of total follow-up, there were no differences on the frequency of stools passage (Human Milk: 3.8 ± 2.4 evacuations per day; Pro + Metlin + Metlos 3.6± 2.0; Pro + Metlin 3.6 ± 2; only Pro 3.4 ± 2.3¸ only formula 3.4 ± 2.0; p NS). Consistency of stools was similar between human milk and prebiotics supplemented groups. Also the frequency of gastrointestinal symptoms was significantly low between these groups. Conclusions. Fructans derivate from agave and added to infant formula are safe and well tolerated by Mexican healthy term babies.


REFERENCES

  1. Norwegian Scientific Committee for Food Safety. Panel on Biological Hazards & Panel on Nutrition, Dietetic Products, Novel Food and Allergy. Risk assessment on use of Lactobacillus rhamnosus (LGG) as an ingredient in infant formula and baby foods (II). Available from: www.vkm.no/dav/63bb45d3eb.pdf [Accessed November 16, 2010].

  2. Norwegian Scientific Committee for Food Safety. Panel on Nutrition, Dietetic Products, Novel Food and Allergy. Risk assessment on use of Lactobacillus rhamnosus (LGG) as an ingredient in infant formula and baby foods (opinion). Available from: www.vkm.no [Accessed November 16, 2010].

  3. European Food Safety Authority. Scientific opinion on the substantiation of a health claim related Immunofortis and strengthening of the baby’s immune system pursuant to Article 14 of Regulation (EC) No 1924/2006. EFSA J 2010; 8: 1430. Available from: http://www.efsa.europa.eu/en/scdocs/doc/ 1430.pdf [Accessed November 16, 2010].

  4. Koletzko B, Bohles H, Fusch C, et al. Recommendations on pre- and probiotics in infant formula [in German]. Monatsschr Kinderheilkd 2009; 157: 267-70.

  5. ESPGHAN Committee on Nutrition. Prebiotic oligosaccharides in dietetic products for infants: a commentary by the ESPGHAN Committee on Nutrition. J Pediatr Gastroenterol Nutr 2004; 39: 465-73.

  6. Braegger C, Chmielewska A, Decsi T, Kolacek S, Mihatsch W, Moreno L, van Goudoever JB. Supplementation of infant formula with probiotics and/or prebiotics: A systematic review and comment by the ESPGHAN Committee on Nutrition. Journal of Pediatric Gastroenterology and Nutrition 2011; 52: 238-50.

  7. Bakker-Zierikzee AM, Alles MS, Knol J, et al. Effects of infant formula containing a mixture of galacto- and fructo-oligosaccharides or viable Bifidobacterium animalis on the intestinal microflora during the first 4 months of life. Br J Nutr 2005; 94: 783-90.

  8. Bakker-Zierikzee AM, Tol EA, Kroes H, et al. Faecal SIgA secretion in infants fed on pre- or probiotic infant formula. Pediatr Allergy Immunol 2006; 17: 134-40.

  9. Brunser O, Figueroa G, Gotteland M, et al. Effects of probiotic or prebiotic supplemented milk formulas on fecal microbiota composition of infants. Asia Pac J Clin Nutr 2006; 15: 368-76.

  10. Alliet P, Scholtens P, Raes M, et al. Effect of prebiotic galactooligosaccharide, long-chain fructo-oligosaccharide infant formula on serum cholesterol and triacylglycerol levels. Nutrition 2007; 23: 719-23.

  11. Arslanoglu S, Moro GE, Boehm G. Early supplementation of prebiotic oligosaccharides protects formula-fed infants against infections during the first 6 months of life. J Nutr 2007; 137: 2420-4.

  12. Arslanoglu S, Moro GE, Schmitt J, et al. Early dietary intervention with a mixture of prebiotic oligosaccharides reduces the incidence of allergic manifestations and infections during the first two years of life. J Nutr 2008; 138: 1091-5.

  13. Ben XM, Zhou XY, Zhao WH, et al. Supplementation of milk formula with galacto-oligosaccharides improves intestinal micro- flora and fermentation in term infants. Chin Med J (Engl) 2004; 117: 927-31.

  14. Ben XM, Li J, Feng ZT, et al. Low level of galacto-oligosaccharide in infant formula stimulates growth of intestinal Bifidobacteria and Lactobacilli. World J Gastroenterol 2008; 14: 6564-8.

  15. Bettler J, Euler AR. An evaluation of the growth of term infants fed formula supplemented with fructo-oligosaccharide. Int J Probiotics Prebiotics 2006; 1: 19-26.

  16. Brunser O, Gotteland M, Cruchet S, et al. Effect of a milk formula with prebiotics on the intestinal microbiota of infants after an antibiotic treatment. Pediatr Res 2006; 59: 451-6.

  17. Costalos C, Kapiki A, Apostolou M, et al. The effect of a prebiotic supplemented formula on growth and stool microbiology of term infants. Early Hum Dev 2008; 84: 45-9.

  18. Decsi T, Arato’ A, Balogh M, et al. Randomised placebo controlled double blind study on the effect of prebiotic oligosaccharides on intestinal flora in healthy infants [in Hungarian]. Orv Hetil 2005; 146: 2445-50.

  19. Fanaro S, Jelinek J, Stahl B, et al. Acidic oligosaccharides from pectin hydrolysate as new component for infant formulae: effect on intestinal flora, stool characteristics, and pH. J Pediatr Gastroenterol Nutr 2005; 41: 186-90.

  20. Haarman M, Knol J. Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula. Appl Environ Microbiol 2005; 71: 2318-24.

  21. Knol J, Scholtens P, Kafka C, et al. Colon microflora in infants fed formula with galacto- and fructo-oligosaccharides: more like breast-fed infants. J Pediatr Gastroenterol Nutr 2005; 40: 36-42.

  22. Magne F, Hachelaf W, Suau A, et al. Effects on faecal microbiota of dietary and acidic oligosaccharides in children during partial formula feeding. J Pediatr Gastroenterol Nutr 2008; 46: 580-8.

  23. Moro G, Minoli I, Mosca M, et al. Dosage-related bifidogenic effects of galacto- and fructooligosaccharides in formula-fed term infants. J Pediatr Gastroenterol Nutr 2002; 34: 291-5.

  24. Moro GE, Mosca F, Miniello V, et al. Effects of a new mixture of prebiotics on faecal flora and stools in term infants. Acta Paediatr Suppl 2003; 91: 77-9.

  25. Moro G, Arslanoglu S, Stahl B, et al. A mixture of prebiotic oligosaccharides reduces the incidence of atopic dermatitis during the first six months of age. Arch Dis Child 2006; 91: 814-9.

  26. Nakamura N, Gaskins HR, Collier CT, et al. Molecular ecological analysis of fecal bacterial populations from term infants fed formula supplemented with selected blends of prebiotics. Appl Environ Microbiol 2009; 75: 1121-8.

  27. Scholtens PA, Alliet P, Raes M, et al. Fecal secretory immunoglobulin A is increased in healthy infants who receive a formula with short-chain galacto-oligosaccharides and long-chain fructo-oligosaccharides. J Nutr 2008; 138: 1141-7.

  28. van Hoffen E, Ruiter B, Faber J, et al. A specific mixture of short-chain galacto-oligosaccharides and long-chain fructo-oligosaccharides induces a beneficial immunoglobulin profile in infants at high risk for allergy. Allergy 2009; 64: 484-7.

  29. Ziegler E, Vanderhoof JA, Petschow B, et al. Term infants fed formula supplemented with selected blends of prebiotics grow normally and have soft stools similar to those reported for breast-fed infants. J Pediatr Gastroenterol Nutr 2007; 44: 359-64.

  30. Lopez MG, Mancilla-Margalli NA, Mendoza-Diaz G. Molecular structures of fructans from Agave tequilana Weber var. azul. J Agric Food Chem 2003; 51(27): 7835-40.

  31. Gomez E, Tuohy KM, Gibson GR, Klinder A, Costabile A. In vitro evaluation of the fermentation properties and potential prebiotic activity of Agave fructans. J Appl Microbiol 2010; 108(6): 2114-21.

  32. Urías-Silvas JE, Cani PD, Delmée E, Neyrinck A, López MG, Delzenne NM. Physiological effects of dietary fructans extracted from Agave tequilana Gto. and Dasylirion spp. Br J Nutr 2008; 99(2): 254-61.

  33. Lohman T, Roche A, Martorell R (eds). Anthropometric standardization reference manual. Illinois, EUA: Human Kinetics Books; 1991.

  34. Habicht JP. Estandarización de métodos epidemiológicos cuantitativos sobre el terreno. Bol Oficina Sanit Panam 1974; 375.

  35. Gibson GR, McCartney AL, Rastall RA. Prebiotics and resistance to gastrointestinal infections. Br J Nutr 2005; 93(Suppl. 1): S31-S34. International Scientific Association for Probiotics and Prebiotics (ISAPP) 6th Annual Meeting. November 9-11, 2008; London, Ontario Canada.

  36. de Vrese M, Schrezenmeir J. Probiotics, prebiotics, and synbiotics. Adv Biochem Eng Biotechnol 2008; 111: 11-66.

  37. Jenkins DJ, Kendall CW, Vuksan V. Inulin, oligofructose and intestinal function. J Nutrition 1999; 129(Suppl. 7): 1431-3.

  38. Ziegler E, Vanderhoof JA, Petschow B, Mitmesser SH, Stolz SI, Harris CL, et al. Term infants fed formula supplemented with blends of prebiotics grow normally and have soft stools similar to those reported for breast-fed infants. J Pediatr Gastroenterol Nutr 2007; 44: 359-64.

  39. Chouraqui JP, Grathwohl D, Labaune JM, Hascoet JM, de Montgolfier I, Leclaire M, Giarre M, et al. Assessment of the safety, tolerance, and protective effect against diarrhea of infant formulas containing mixtures of probiotics or probiotics and prebiotics in a randomized controlled trial. Am J Clin Nutr 2008; 87(5): 1365-73.

  40. Savino F, Palumeri E, Castagno E, Cresi F, Dalmasso P, Cavallo F, Oggero R. Reduction of crying episodes owing to infantile colic: a randomized controlled study on the efficacy of a new infant formula. European Journal of Clinical Nutrition 2006; 60: 1304-10.




2020     |     www.medigraphic.com