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2005, Number 2

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Vet Mex 2005; 36 (2)

Effect of the caprine arthritis encephalitis virus in the reproductive system of male goats

Martínez RHA, Ramírez ÁH, Tórtora PJ, Aguilar SA, Garrido FGI, Montaraz CJA

Language: English/Spanish
References: 34
Page: 159-176
PDF size: 553.80 Kb.


ABSTRACT

The effect of the Caprine Arthritis Encephalitis Virus (CAEV) on the reproductive system of the male goat was evaluated. Fourteen adult males distributed into 4 groups were studied: I) negative control (uninfected animals) (n=3); II) animals experimentally infected with an autochthonous CAEV (FES-C.UNAM isolate) (n=3); III) naturally infected animals (n=5); IV) animals experimentally infected with CAEV reference strain from the American Type Culture Collection (ATCC) (n=3). Blood and semen samples were collected every 30 days and animals were clinically evaluated (especially for possible external genital alterations) during 10 months. No differences in scrotal diameter, seminal motility, seminal pH, color and volume were found among the four groups. Antibodies against CAEV were detected by ELISA in blood serum and seminal fluid in animals infected with the autochthonous strain after 7 months, in animals experimentally infected with the reference strain after 3 months and animals naturally infected remained positive throughout the experiment. By Western Blot, antibodies against proteins p14, p16, p19, p25, p27, p45, p71, p90 and gp135 thousand kDa were found in seminal fluid and in blood serum against proteins p16, p19, p25, p27, p71, p90, and gp135 thousand kDa. Animals were humanely sacrificed 10 months after inoculation. No pathological alterations were found in the genital organs of animals belonging to groups I and IV. In group II only one animal was found with a granuloma in the head of the epididymis of the right testis with a yellowish creamy substance. In group III one animal was found with hydrocele that contained a serous liquid, another with edema in the head of the right testis and another one with syncytia in seminal vesicle. Immunohistochemical studies showed CAEV antigens in epithelial cells of the seminal vesicles, ampulla and bulbourethral glands in groups II, III and IV. By PCR, CAEV was detected in seminal fluid at the time of sacrifice. No histological alterations were found except in one animal belonging to group III which presented syncytia in the seminal vesicle. The presence of antigen in the tissues demonstrates that these may act as disseminators of CAE.


REFERENCES

  1. Rowe J D, East N E. Risk factors for transmission and methods for control of caprine arthritis-encephalitis virus infection. Vet Clin North Am Food Anim Retrovirus Pract 1997; 13 (I): 25-53.

  2. East N E, Rowe JD, Dahlberg JE, Theilen GH, Pedersen NC. Modes of transmission of caprine arthritis encephalitis virus infection.Small Rumin Res 1993;10: 251-261.

  3. Martinez HA, Ramirez AH, Tortora PJL, Montaraz CJA. Detection of antibodies in seminal liquid of animals infected with goats arthritis encephalitis by western blotting. International Conference and Workshop on animals Retroviruses 2000 September 3-5. Queens´ College Cambridge UK: 62.

  4. Novelo BP, Martinez RHA, Tortora PJ, Ramirez AH. Serologic diagnosis of caprine arthritis encephalitis using an immunodiffusion test on bucks. 7th International Conference on goats 2000 May 15-21 Tours. France. 819 pp. Round. Table 3. Detection and prevention of CAE. International Goats Association 2000. Proceedings. Tome II. Nouzilly. France.37380.

  5. Travassos C, Benoit C, Valas S, Da Silva A, Perrin G. Détection du virus de l´arthritis encéfalite caprine dans le sperma de boucs infectés expérimentalement. Vet Res 1998; 29:578-579.

  6. Travassos CE, Benoit C., Valas S, Da Silva AG, Perrin G. Caprine arthritis-encephalitis virus in semen of naturally infected bucks. Small Rumin Res 1999; 32:101-106.

  7. Van Voorhis BJ, Martinez A, Mayer K, Anderson DJ. Detection of Human immunodeficiency virus type 1 in semen from seropositive men using culture and polimerasa chain reaction deoxyribonucleic acid amplification techniques. Fertil Steril 1991; 55:588-594.

  8. Nash JW, Hanson LA, Coats KSC. Bovine immunodeficiency virus in stud bull semen. Am J Vet Res 1995; 56:760-763.

  9. De la Concha-Bermejillo A, Magnus-Corral S, Brodie SJ, Demartin JC. Venereal shedding of ovine lentivirus in infected rams. Am J Vet Res 1996; 57: 684-688.

  10. Jordan HL, Liang Y, Hudson LC, Tompkins WA. Shedding of feline immunodeficiency virus in semen of domestic cats during acute infection. Am J Vet Res 1999; 60:211-215.

  11. Nazara SJ, Trigo FJ, Suberbie E, Madrigal V. Estudio serológico de la artritis encefalitis caprina en México.Téc Pec Méx 1985; 48:99-101.

  12. Vitu C, Russo P, Vignoni M, Arthrite-Encephalite caprine: Essai D´une preparation vaccinale adjuvee-II. Etude de la reponse anticorps. Com Immun Microbiol Infect Dis 1993;2:137-144.

  13. Russo P, Vitu C, Fontaine JJ, Vignoni M. Arthrite- encéfalite caprine : Essai d´une préparation vaccinale adjuvée.I Étude clinique et virologique. Com Immun Microbiol Infect Dis 1993;16:131-136.

  14. Beyer JC, Chebloune Y, Lakhal-Mselli L, Hötzel I, Mcwhirter-Kumpula N, Cheevers WP. Immunization with plasmad DNA expressing the caprine arthritis-encephalitis virus envelope gene. Quantitative and qualitative aspects of antibodies response to viral surface glycoprotein. Vaccine 2001; 19:1643-1651.

  15. Foote RH. Value of testicular and sperm profiles in optimizing reproductive success: lessons learned from selective breeding programs of domestic and laboratory animals. Sperm measures and reproductive success. Institute for Healt Policy Analysis. Forum on Science health and environmental risk assessment. In:Burger Jr EJ, Tardiff RG, Scialli AR, Zenick H, editors. Progress in Clinical and Biological Research 32. New York, Ithaca. Alan R. Liss, Inc. 1989:107-126.

  16. Laemmli VK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-685.

  17. Clavijo A, Thorsen J. Chemilumininescent detection of caprine arthitis encephalitis virus with a PCR-generated single stranded non radiolabelled probe. Vet Microbiol 1995; 43:295-305.

  18. Miller CJ, Vogel P, Alexander NJ, Dandekar AS, Hendrickx AG, MarxP A. Pathology and localization of simian immunodeficiency virus in the reproductive tract of chronically infected male Rhesus macaques. Lab Invest 1994; 70:255-262.

  19. Ellis TM, Wilcox GE, Robinson WF. Antigenic variation of caprine arthritis encephalitis virus during persistent infection of goats. J Gen Virol 1987; 68:3145-3152.

  20. Adams DS, Klevjer-Anderson P, Carlson JL, McGuire TC, Gorham JR. Transmission and control of caprine artritis-encephalitis virus. Am J Vet Res 1983; 44:837-840.

  21. Cheveers WP, Knowles DP, McGuire TC, Cunningham DR, Adams DS, Gorham JR. Chronic disease in goats orally infected with two isolates of the caprine arthritis encephalitis lentivirus. Lab Invest 1988; 58: 510-517.

  22. LechnerF, Machado J, Bertoni G, Seow HF, Dobbelaere DA, Peterhans E. Caprine arthritis encephalitis virus dysregulates the expression of cytokines in macrophages. J Virol 1997; 71:7488-7497.

  23. Adeyemo O, Gao RJ, Lan HC. Cytoquine production in vitro by macrophages of goats with caprine arthritis encephalitis. Cell Mol Biol 1997; 43:1031-1037.

  24. York DF, Vigne R, Verwoerd DW, Querat G. Nucleotide sequence of jaagsiekte retrovirus, an exogenous and endogenous type D and B retrovirus of sheep and goats. J Virol 1992; 66:4930-4939.

  25. Belov L, Whalley MJ. Virus-specific polipeptides of caprine arthritis encephalitis virus recognized by monoclonal antibodies to virion protein p24 and p14. J Gen Virol 1988;69:1097-1103.

  26. Meas S, Usui T, Ohashi K, Sugimoto Ch, Onuma M. Vertical transmission of bovine leukemia virus and bovine immunodeficiency virus in dairy cattle herds. Vet Microbiol 2002; 84:275-282.

  27. Kashuba ADM, Dyer JR, Kramer LM, Raasch RH, Eron JJ, Cohens MS. Antiretroviral-Drugs concentration in semen: implications for sexual transmission of human immunodeficiency virus type 1. Antimicrob Agents Chemother 1999; 43:1817-1826.

  28. Kiessling AA, Crowell RC, Connell RS. Sperm-associated retrovirus in the mouse epididymis. Proc Natl Acad Sci USA 1987; 84:8667-8671.

  29. Mselli-Lakhal L, Guiguen F, Fornazero C, Du J, Favier C, Durand J, et al. Goat milk epithelial cells are permissive to CAE infection in vitro. Virology 1999; 259:67-73.

  30. Jan CL, Greenland T, Gounel F, Balleydier S, Mornex JF. Activation of small ruminant aortic endothelial cells after in vitro infection by caprine arthritis encephalitis virus. Res Vet Sci 2000; 69:225-231.

  31. Lamara A, Fieni F, Mselli-Lakhal L, Taintuier D, Chebloune Y. Efficient replication of caprine arthritis-encephfalitis virus in goats granulosa cells. Virus Res 2001; 79:165-172.

  32. Zacharopoulus VR, Phillips DM. Cell-mediated HTLV-I infection of a cervix-derived epithelial cell-line. Microb Pathog 1997; 23:225-233.

  33. Pudney J, Anderson D. Orchitis and human immunodeficiency virus type 1 infected cells in reproductive tissues from men with the acquired immune deficiency syndrome. Am J Pathol 1991; 139:149-161.

  34. Ali O A. Caprine arthritis-encephalitis related changes in the uterus of a goat. Vet Rec 1987;121:131-132.




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