medigraphic.com
ISSN 1561-2996 (Electronic)
ISSN 0864-0289 (Print)

2013, Number 2

<< Back Next >>

Rev Cubana Hematol Inmunol Hemoter 2013; 29 (2)

Distribution of types of paraproteins in a sample of patients with multiple myeloma

Arce-Hernández AA, Villaescusa-Blanco R, Morera-Barrios LM, Junco-González Y, Merlín-Linares JC, Ramón-Rodríguez L, Hernández-Padrón C

Language: Spanish
References: 19
Page:
PDF size: 49.29 Kb.


ABSTRACT

Introduction: multiple mieloma (MM) is a disease characterized by a monoclonal proliferation of immunoglobulins representing approximately 15 % of malignant hemopathies.
Methods: the distribution of classes, subclasses and light chains of monoclonal immunoglobulins was studied in 285 patients with MM. Three methods were used: serum protein electrophoresis for the detection of monoclonal immunoglobulins or paraproteins, immunofixation electrophoresis and double immunodiffusion to identify classes, subclasses and light chain types.
Results: 206 patients (72.28 %) with IgG MM, 73 (25,62 %) with IgA MM, and 6 (2,1 %) with IgM MM were found. The distribution of IgG subclasses was: 130 cases (63,11 %) IgG1; 43 (20,87 %) IgG2; 21 (10,19 %) IgG3: and 12 (5,83 %) IgG4. Distribution of IgA subclasses was: 59 patients (80,82 %) IgA1 and 14 (19,18 %) IgA2; 187 patients (65,62 %) showed kappa light chains and 98 (34,38 %) were lambda.
Conclusions: the data obtained in our study allowed us to identify the frequency of distribution of classes, subclasses and light chains in a sample of patients with MM.


REFERENCES

  1. Palumbo A, Anderson K. Multiple myeloma. N Engl J Med. 2011;364(11):1046-60.

  2. Conté G, Gastón M, Lois V, Cabrera ME, León A, García H, et al. Mieloma Múltiple en Chile. Características clínicas y sobrevida. Rev Méd Chile. 2007;135(9):1111 7.

  3. Kyle RA, Gertz MA, Witzig TE, Lust JA, Lacy MQ, Dispenzieri A, et al. Review of 1027 patients with newly diagnosed multiple myeloma. Mayo Clinic Proceedings. 2003 Jan;78(1):21-3.

  4. Withold M, Rick W. An immunoblotting procedure following agarose gel electrophoresis for subclass typing of IgG paraproteins in human sera. Eur J Clin Chem Clin Biochem. 1993 Jan;31(1):17-21.

  5. Klouche M, Bradwell AR, Wilhelm D, Kirchner H. Subclass typing of IgG paraproteins by immunofixation electrophoresis. Clin Chem. 1995 Oct;41(10):1475-9.

  6. Durrum EL. Paper electrophoresis. En:Eleck RJ, Durrum EL, Zweig G (eds). Paper chromatography and paper electrophoresis. 2nd ed. New York:Academic. 1958;489- 674.

  7. D´Agostino LE, Marcuzzo GD. Immunofixation: complement of immunoelectrophoresis in the study of monoclonal immunoglobulins. Acta Bioquim Clin Latinoam. 1987 dic; 21(4):477-82.

  8. Ouchterlony O, Nilsson LA. Immunodiffusion and immunoelectrophoresis. En: Weir DM (ed). Handbook of Experimental Immunology. Oxford: Blackwell Scientific Publications. 1986; p.32.

  9. Baldini L, Guffanti A, Cesana BM, Colombi M, Chiobli O, Damilano I, et al. Role of different hematologic variables in defining the risk of malignant transformation in monoclonal gammopathy. Blood. 1996 Feb;87(3):912-8.

  10. Bartl R. Monoclonal immunoglobulins. In: Bartl R, Wilma A, Lothar M, Thomas E, eds. Clinical laboratory Diagnostics. York: Deighton. 2001;742-58.

  11. Schuster SR, Rajkumar SV, Dispenzieri A, Morice W, Aspitia AM, Ansell S et al. IgM multiple myeloma definition, prognosis and differentiation from Waldenström macroglobulinemia Am J Hematol. 2010 Nov;85(11):853-5.

  12. Vijay A, Gertz M. Waldenström macroglobulinemia. Blood. 2007 Jun;109(12):5096-103.

  13. Avet-Loiseau H, Attal M, Moreau P, Charbonnel C, Garban F, Hulin C, et al Genetic abnormalities and survival in myeloma multiple.: the experience of the Intergroupe Francophone du Myélome. Blood. 2007 Apr;109(8):3489-95.

  14. Bergsagel PL, Kuehl WM. Chromosome translocations in multiple myeloma. Oncogene. 2001;20(40):5611-22.

  15. Fonseca R, Barlogie B, Bataille R, Bastard C, Bergsagel PL, Chesi M, et al. Genetics and cytogenetics of multiple myeloma: a workshop report. Cancer Res. 2004; 64(4):1546-58.

  16. Fonseca R, Bailey RJ, Ahmann GJ, Rajkumar SV, Hoyer JD, Lust JA, et al. Genomic abnormalities in monoclonal gammopathy of undetermined significance. Blood. 2002;100 (4):141724.

  17. Avet-Loiseau H, Facon T, Grosbois B, Magrangeas F, Rapp MJ, Harousseau JL, et al. Oncogenesis of multiple myeloma: 14q32 and 13q chromosomal abnormalities are not randomly distributed, but correlate with natural history, immunological features, and clinical presentation. Blood. 2002;99(6):218591.

  18. Fasullo FJ, Frische HA, Liu FJ, Hamilton RG. IgG heavy-chian subclases typing of mieloma paraproteins by isoelectric focusing immunoblot analysis. Clin Chem. 1989;35:364-8.

  19. Gharagozloo S, Khoshnoodi SRA, Shokri F. Distribution of immunoglobulin light and heavy chain and subclasses in paraproteins isolated from Iranian patients with multiple myeloma. Irn J Med Sci. 1996;21:105-11.




2020     |     www.medigraphic.com