López NJA, Ríos MS, Chávez-Olivares VMA, Serrano NOS

Language: Spanish
References: 22
Page: 186-191
PDF size: 926.11 Kb.

ABSTRACT

Background: With the introduction of the MSOME (Motile Sperm Organelle Morphology Examination), prospective, retrospective and/or randomized studies, have been developed, in order to evaluate the benefits that this methodology can offer among the results of a reproductive center. There are different optics for sperm selection (Hoffman or Nomarski), in order to improve the rates of fertilization and embryo quality. When the spermatozoa present vacuoles in more than 20% or great size vacuoles, embryos with low probability of implantation are produced.
Objective: To compare the diagnosis by MSOME and morphology described in manual of WHO, 2010 of spermatozoa nucleus of the infertile men, who, after low or high complexity assisted reproduction procedures, did not achieve the pregnancy or was produced a loss of pregnancy.
Patient and method: 50 males included in low or high complexity protocols of Centro Mexicano de Fertilidad, a basic semen analysis was done, and results were classified according to the “WHO laboratory manual for the examination and processing of human semen” (WHO, 2010). MSOME was done immediately to diagnose alterations of the sperm organelle.
Results: It was determined in the semen analysis that: 17 patients (35%) presented asthenozoospermia, 11 (19%) oligozoospermia, 10 (21%) teratozoospermia and 12 (25%) oligoasthenoteratozoospermia. MSOME results shown that in sperm of patients diagnosed with teratozoospermia and oligoasthenoteratozoospermia more than the 20% of the spermatozoa presented vacuoles. Even though vacuoles were observed in sperm samples of those patients diagnosed with asthenozoospermia and oligozoospermia, the concentration was less than 20%.
Conclusions: Optimizing gametes selection is one of the best tools for the assisted reproduction laboratory. To analyze sperm with MSOME technique before including patients to low and high complexity procedures is a very useful diagnosis tool to improve pregnancy rates.

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