medigraphic.com
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2012, Number 1

<< Back Next >>

Rev Invest Clin 2012; 64 (1)

CSF RT-PCR standardization in pediatric patients with enteroviral central nervous system infection

García-Elorriaga G, Esparza-García A, Méndez-Rojas C, del Rey-Pineda G, González-Bonilla C

Language: Spanish
References: 33
Page: 59-66
PDF size: 215.73 Kb.


ABSTRACT

Objective. We standardized the RT-PCR panviral CSF and determined its applicability in detecting acute enterovirus infection in the central nervous system in children under 15 years. Material and methods. RT-PCR was performed directly in CSF samples of 10 pediatric patients with suspected CNS infection and 9, with different conditions of the central nervous system. Results. 80% (8/10) of RT-PCR samples were positive for enterovirus in patients with suspected CNS infection and no sample was positive in patients with different ailments. Conclusions. Since enteroviruses are among the main etiologies of pediatric encephalitis, RT-PCR could be particularly useful for rapid detection in CSF.


REFERENCES

  1. Stanway G, Brown F, Christian P, Hovi T, Hyypia T, King AMQ, et al. Family Picornaviridae. In: Fauquet CM, Mayo MA, Maniloff J, U Desselberger, Ball LA (ed.). Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. London, England: Elsevier/Academic Press; 2005, p. 757-78.

  2. Brown B, Oberste MS, Maher K, Pallansch MA. Complete genomic sequencing shows that polioviruses and members of human enterovirus species C are closely related in the noncapsid region. J Virol 2003; 77: 8973-84.

  3. Melnick JL. Enteroviruses: polioviruses, coxackieviruses, ECHO viruses and newer enteroviruses. In: Fields BN (ed.). Virology. New York: Raven Press; 1990, p. 549-605.

  4. Lukashev AN, Ivanova OE, Khudiakova LV. Social and economic significance of enterovirus infection and its role in etiologic structure of infectious diseases in the world. Zh Mikrobiol Epidemiol Immunobiol 2010; 5: 113-20.

  5. Centers for Disease Control and Prevention (CDC). Nonpolio enterovirus and human parechovirus surveillance-United States, 2006-2008. MMWR 2010; 59(48): 1577-80.

  6. Modlin JF. Enterovirus infection in infants and children. Adv Pediatr Infect Dis 1999; 12: 155-8.

  7. Rotbart HA. Enteroviral infections of the central nervous system. Clin Infect Dis 1995; 20: 971-81.

  8. Coria-Lorenzo JJ, Juárez-Escobar M, Velasco-Alvarez VH. Meningoencefalitis viral. Enfoque clínico. Rev Mex Ped 2001; 68: 252.

  9. Lamaräo LM, Gomes M de L, Ferreira LL, Fonseca CM, Araujo LC, Santan MB, et al. Investigation of enterovirus in case of aseptic meningitis syndrome of Belem. Rev Soc Bras Med Trop 2005; 38: 391-5.

  10. Steiner I, Budka H, Chaudhuri A, Koskiniemi M, Sainio K, Salonen O, et al. Viral meningoencephalitis: a review of diagnostic methods and guidelines for management. Eur J Neurol 2010; 17(8): 999-e57.

  11. Lin M, Chan Y, Chen C, Tang R, Wu K. Epidemiology and diagnostic methodology for enterovirus infection, including cerebrospinal fluid analysis: a 5-year review. J Microbiol Infect 2009; 41(6): 450-5.

  12. Leitch E, Harvala H, Robertson I, Ubillos I, Templeton K, Simminds P. Direct identification of human enterovirus serotypes in cerebrospinal fluid by amplification and sequencing of the VP1 region. J Clin Virol 2009; 44(2): 119-24.

  13. Hong J, Kang B, Kim A, Hwang S, Lee S, Kim J, et al. Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology. J Clin Microbiol 2010; 48(2): 615-6 [Doi:10.1128/ JCM.01790-09].

  14. Pozzetto B, Andreoletti L, Beguier E, Bourlet T, Dussaix E, Grangeot-Keros L, et al. Multicenter evaluating of a commercially available PCR assay for diagnosing enterovirus infection in a panel of cerebrospinal fluid specimens. J Clin Microbiol 1996; 34: 3002-6.

  15. Young PP, Buller RS, Storch GA. Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens. J Clin Microbiol 2000; 39: 4260-1.

  16. Peigue-Lafeuille H, Archimbaud C, Mirand A, Chambon M, Regagnon C, Laurichesse H, et al. From prospective molecular diagnosis of enterovirus meningitis to prevention of antibiotic resistance. Med Mal Infect 2006; 36: 124-31.

  17. Read SJ, Jeffery KJM, Bangham CRM. Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory. J Clin Microbiol 1997; 35: 691-6.

  18. Rotbart HA, Sawyer MH, Fast S, Lewinski C, Murphy N, Keyser EF, et al. Diagnosis of enteroviral meningitis using PCR with a microwell detection assay. J Clin Microbiol 1994; 32: 2590-2.

  19. Vuorinen T, Vanionpaa R, Hyypia T. Five years’ experience of reverse-transcriptase polymerase chain reaction in daily diagnosis of enterovirus and rhinovirus infections. Clin Infect Dis 2003; 37: 452-5.

  20. Zoll GJ, Melchers WJF, Kopecka H, Jambroes G, Van der Piel HJA, Galama JMD. General primer-mediated polymerase chain reaction for detection of enterovirus: application for diagnostic routine and persistent infections. J Clin Microbiol 1992; 30: 160-5.

  21. Lee BE, Davies HD. Aseptic meningitis. Curr Opin Infect Dis 2007; 20(3): 272-7.

  22. Archimbaud C, Chambon M, Bailly JL, Petit I, Henquell C, Mirand A, et al. Impact of rapid enterovirus molecular diagnosis on the management of infants, children, and adults with aseptic meningitis. J Med Virol 2009; 81(1): 42-8.

  23. Chomczynski P, Hulley SB, Cummings SR. Designing Clinical Research: an epidemiological approach. Baltimore, Marylan; Williams and Wilkins; 1998, p. 325-32.

  24. Iizuka N, Kuge S, Nomoto A. Complete nucleotide sequence of the genome of coxsackie B1. Virology 1987; 156: 64-73.

  25. Narkeviciute I, Vaiciuniene D. Outbreak of echovirus 13 infection among Lithuanian children. Clin Microbiol Infect 2004; 10(11): 1023-5.

  26. Silverman R. The Pleconaril Pediatric Meningitis Study Group: frequency of enteroviral meningitis among children presenting to the emergency department with viral/aseptic meningitis by using a PCR assay. Pediatrics 1999; 34(4, Part 2) Suppl.: S9-S10.

  27. Davis L, Tyler K. Molecular diagnosis of CNS viral infections. J Neurol Neurosurg Psychiatry 2005; 76(1): 10.

  28. Fu J, Yuan Y, Sun L, Cui X. Relation between acute respiratory infection and enterovirus in children in Beijing area. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2007; 21(4): 316-8.

  29. Chokephaibulkit K, Kankirawatana P, Apintanapong S, Pongthapisit V, Yoksan S, Kositanont U, et al. Viral etiologies of encephalitis in Thai children. Pediatr Infect Dis J 2001; 20(2): 216-8.

  30. Brown D, Irish G, Robinson R, Swan A, Banatvala J, Howard R, et al. Diagnosis and outcomes of enterovirus infections in young infants. Pediatr Infect Dis J 2005; 24: 546-50.

  31. Fowler A, Stödberg T, Eriksson M, Wickström R. Childhood encephalitis in Sweden: etiology, clinical presentation and outcome. Eur J Paediatr Neurol 2008; 12(6): 484-90.

  32. Sarmiento L, Mas P, Goyeneçhea A, Palomera R, Morier L, Capó V, et al. First epidemia of echovirus 16 meningitis in Cuba. Emerging Infectious Dis 2001; 7: 887-9.

  33. Petitjean J, Quibriac F, Freymuth F, Fuchs N, Laconche N, Aymard M, et al. Specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes. J Clin Microbiol 1990; 28: 307-11.




2020     |     www.medigraphic.com