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Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

2012, Número 1

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Rev Invest Clin 2012; 64 (1)


Estandarización de RT-PCR en LCR de pacientes pediátricos con infección del sistema nervioso central por enterovirus

García-Elorriaga G, Esparza-García A, Méndez-Rojas C, del Rey-Pineda G, González-Bonilla C

Idioma: Español
Referencias bibliográficas: 33
Paginas: 59-66
Archivo PDF: 215.73 Kb.


RESUMEN

Objetivo. Estandarizar la RT-PCR panviral en LCR y determinar su aplicabilidad para detectar infección aguda por enterovirus en el sistema nervioso central en menores de 15 años. Material y métodos. Se realizó RT-PCR directamente en muestras de LCR de diez pacientes pediátricos con sospecha de infección del sistema nervioso central y en nueve con padecimientos diferentes del sistema nervioso central. Resultados. El 80% (8/10) de las muestras fueron RT-PCR positivas para enterovirus en los pacientes con sospecha de infección del sistema nervioso central; ninguna muestra resultó positiva en los pacientes con padecimientos diferentes. Conclusiones. Ya que los enterovirus se encuentran entre las principales etiologías de encefalitis pediátrica, la RT-PCR podría ser particularmente útil para su detección rápida en LCR.


REFERENCIAS (EN ESTE ARTÍCULO)

  1. Stanway G, Brown F, Christian P, Hovi T, Hyypia T, King AMQ, et al. Family Picornaviridae. In: Fauquet CM, Mayo MA, Maniloff J, U Desselberger, Ball LA (ed.). Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses. London, England: Elsevier/Academic Press; 2005, p. 757-78.

  2. Brown B, Oberste MS, Maher K, Pallansch MA. Complete genomic sequencing shows that polioviruses and members of human enterovirus species C are closely related in the noncapsid region. J Virol 2003; 77: 8973-84.

  3. Melnick JL. Enteroviruses: polioviruses, coxackieviruses, ECHO viruses and newer enteroviruses. In: Fields BN (ed.). Virology. New York: Raven Press; 1990, p. 549-605.

  4. Lukashev AN, Ivanova OE, Khudiakova LV. Social and economic significance of enterovirus infection and its role in etiologic structure of infectious diseases in the world. Zh Mikrobiol Epidemiol Immunobiol 2010; 5: 113-20.

  5. Centers for Disease Control and Prevention (CDC). Nonpolio enterovirus and human parechovirus surveillance-United States, 2006-2008. MMWR 2010; 59(48): 1577-80.

  6. Modlin JF. Enterovirus infection in infants and children. Adv Pediatr Infect Dis 1999; 12: 155-8.

  7. Rotbart HA. Enteroviral infections of the central nervous system. Clin Infect Dis 1995; 20: 971-81.

  8. Coria-Lorenzo JJ, Juárez-Escobar M, Velasco-Alvarez VH. Meningoencefalitis viral. Enfoque clínico. Rev Mex Ped 2001; 68: 252.

  9. Lamaräo LM, Gomes M de L, Ferreira LL, Fonseca CM, Araujo LC, Santan MB, et al. Investigation of enterovirus in case of aseptic meningitis syndrome of Belem. Rev Soc Bras Med Trop 2005; 38: 391-5.

  10. Steiner I, Budka H, Chaudhuri A, Koskiniemi M, Sainio K, Salonen O, et al. Viral meningoencephalitis: a review of diagnostic methods and guidelines for management. Eur J Neurol 2010; 17(8): 999-e57.

  11. Lin M, Chan Y, Chen C, Tang R, Wu K. Epidemiology and diagnostic methodology for enterovirus infection, including cerebrospinal fluid analysis: a 5-year review. J Microbiol Infect 2009; 41(6): 450-5.

  12. Leitch E, Harvala H, Robertson I, Ubillos I, Templeton K, Simminds P. Direct identification of human enterovirus serotypes in cerebrospinal fluid by amplification and sequencing of the VP1 region. J Clin Virol 2009; 44(2): 119-24.

  13. Hong J, Kang B, Kim A, Hwang S, Lee S, Kim J, et al. Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology. J Clin Microbiol 2010; 48(2): 615-6 [Doi:10.1128/ JCM.01790-09].

  14. Pozzetto B, Andreoletti L, Beguier E, Bourlet T, Dussaix E, Grangeot-Keros L, et al. Multicenter evaluating of a commercially available PCR assay for diagnosing enterovirus infection in a panel of cerebrospinal fluid specimens. J Clin Microbiol 1996; 34: 3002-6.

  15. Young PP, Buller RS, Storch GA. Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens. J Clin Microbiol 2000; 39: 4260-1.

  16. Peigue-Lafeuille H, Archimbaud C, Mirand A, Chambon M, Regagnon C, Laurichesse H, et al. From prospective molecular diagnosis of enterovirus meningitis to prevention of antibiotic resistance. Med Mal Infect 2006; 36: 124-31.

  17. Read SJ, Jeffery KJM, Bangham CRM. Aseptic meningitis and encephalitis: the role of PCR in the diagnostic laboratory. J Clin Microbiol 1997; 35: 691-6.

  18. Rotbart HA, Sawyer MH, Fast S, Lewinski C, Murphy N, Keyser EF, et al. Diagnosis of enteroviral meningitis using PCR with a microwell detection assay. J Clin Microbiol 1994; 32: 2590-2.

  19. Vuorinen T, Vanionpaa R, Hyypia T. Five years’ experience of reverse-transcriptase polymerase chain reaction in daily diagnosis of enterovirus and rhinovirus infections. Clin Infect Dis 2003; 37: 452-5.

  20. Zoll GJ, Melchers WJF, Kopecka H, Jambroes G, Van der Piel HJA, Galama JMD. General primer-mediated polymerase chain reaction for detection of enterovirus: application for diagnostic routine and persistent infections. J Clin Microbiol 1992; 30: 160-5.

  21. Lee BE, Davies HD. Aseptic meningitis. Curr Opin Infect Dis 2007; 20(3): 272-7.

  22. Archimbaud C, Chambon M, Bailly JL, Petit I, Henquell C, Mirand A, et al. Impact of rapid enterovirus molecular diagnosis on the management of infants, children, and adults with aseptic meningitis. J Med Virol 2009; 81(1): 42-8.

  23. Chomczynski P, Hulley SB, Cummings SR. Designing Clinical Research: an epidemiological approach. Baltimore, Marylan; Williams and Wilkins; 1998, p. 325-32.

  24. Iizuka N, Kuge S, Nomoto A. Complete nucleotide sequence of the genome of coxsackie B1. Virology 1987; 156: 64-73.

  25. Narkeviciute I, Vaiciuniene D. Outbreak of echovirus 13 infection among Lithuanian children. Clin Microbiol Infect 2004; 10(11): 1023-5.

  26. Silverman R. The Pleconaril Pediatric Meningitis Study Group: frequency of enteroviral meningitis among children presenting to the emergency department with viral/aseptic meningitis by using a PCR assay. Pediatrics 1999; 34(4, Part 2) Suppl.: S9-S10.

  27. Davis L, Tyler K. Molecular diagnosis of CNS viral infections. J Neurol Neurosurg Psychiatry 2005; 76(1): 10.

  28. Fu J, Yuan Y, Sun L, Cui X. Relation between acute respiratory infection and enterovirus in children in Beijing area. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2007; 21(4): 316-8.

  29. Chokephaibulkit K, Kankirawatana P, Apintanapong S, Pongthapisit V, Yoksan S, Kositanont U, et al. Viral etiologies of encephalitis in Thai children. Pediatr Infect Dis J 2001; 20(2): 216-8.

  30. Brown D, Irish G, Robinson R, Swan A, Banatvala J, Howard R, et al. Diagnosis and outcomes of enterovirus infections in young infants. Pediatr Infect Dis J 2005; 24: 546-50.

  31. Fowler A, Stödberg T, Eriksson M, Wickström R. Childhood encephalitis in Sweden: etiology, clinical presentation and outcome. Eur J Paediatr Neurol 2008; 12(6): 484-90.

  32. Sarmiento L, Mas P, Goyeneçhea A, Palomera R, Morier L, Capó V, et al. First epidemia of echovirus 16 meningitis in Cuba. Emerging Infectious Dis 2001; 7: 887-9.

  33. Petitjean J, Quibriac F, Freymuth F, Fuchs N, Laconche N, Aymard M, et al. Specific detection of enteroviruses in clinical samples by molecular hybridization using poliovirus subgenomic riboprobes. J Clin Microbiol 1990; 28: 307-11.




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