Decrease of pro-inflammatory cytokines and regulation of Toll-like receptors 2 and 4 expression in neutrophils and monocytes after the administration of a symbiotic on patients with infection by HIV

Luz Alicia González-Hernández; Mary Fafutis-Morris; Karina Esthela Montes-Salcedo; Luis Giovani Valle-Gutiérrez; Jaime Federico Andrade-Villanueva

2011, Number 2

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Rev Med MD 2011; 2.3 (2)

Decrease of pro-inflammatory cytokines and regulation of Toll-like receptors 2 and 4 expression in neutrophils and monocytes after the administration of a symbiotic on patients with infection by HIV

González-Hernández LA, Fafutis-Morris M, Montes-Salcedo KE, Valle-Gutiérrez LG, Andrade-Villanueva JF

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Language: Spanish
References: 19
Page: 60-66
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ABSTRACT

Background: The human immunodeficiency virus (HIV) induces an immune dysfunction propitiating the onset of HIV associated enteropathy. Neither the benefits nor the safety of the use of probiotics on patients with HIV have been studied.
Objective: To assess whether the intestinal microbiota modification with the use of symbiotic regulates the TLR 2 and 4 expression in peripheral blood monocytes and neutrophils. Moreover, to provide evidence of a diminution of plasmatic proinflammatory cytokines.
Methods: Randomized clinical trial, double blind, performed in the Unit of HIV/AIDS in Guadalajara's “Fray Antonio Alcalde” Civil Hospital and the Centro de investigación en Inmunología y Dermatología in the Health sciences campus of the University of Guadalajara during 2008 to 2010. 20 patients were included and distributed in 4 groups of 5 individuals with infection by HIV-1 without retroviral treatment and no admission criteria (Stage ≥ B), with CD4⁺T cells > 350 cells/µ L. The group A was assigned to received the symbiotic, the group B a probiotic, the group C a prebiotic, and the group D a placebo. The concentrations of cytokines in plasma were quantified (IL-1β, IL-10 and TNF-α) and the TLR 2 and 4 expression in monocytes and neutrophils through flow cytometry.
Results: In the group A, was observed a decrease in the concentration of IL-6 (p‹ 0.05). For the IL-1β, IL-10 and TNF-α concentrations, no significant difference was observed. The TLR-4 expression in neutrophils and monocytes presented an increase. However, it was not a statistical difference (p=0.056). In the group B, no significant statistical differences were observed for IL-6, TNF-α, IL-1β and IL-10. The TLR-2 presented a significant statistical increase in expression in neutrophils (p=‹ 0.016), but with no significant changes in monocytes. In the groups C and D, there was no significance on the concentration of IL-1β, IL-10 and TNF-α. However, the placebo group had a significant negative correlation, between T cells CD4⁺and IL-6 concentration (r =-1.0, p =0.01).
Conclusions: The use of symbiotic diminishes the levels of IL-6 on patients with HIV who have not yet developed AIDS showing safety on use.

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