2011, Number 3
<< Back Next >>
Biotecnol Apl 2011; 28 (3)
Low genetic variability in the fifth introduction of Litopenaeus vannamei in Cuba, as estimated with microsatellite markers
Artiles A, Rodríguez I, Pérez A, Pérez L, Espinosa G
Language: Spanish
References: 22
Page: 147-150
PDF size: 166.61 Kb.
ABSTRACT
The present work estimated the genetic variability and relatedness index of the fifth stock of Pacific white shrimp, Litopenaeus vannamei, imported into Cuba for farming purposes from the US Shrimp Improvement System (SIS). Genetic variability was estimated by genotyping 33 samples for four microsatellite loci: M1, Pvan 1815, Pvan 0040 and Pvan 1758. This stock had average expected and observed heterozygosities of 0.37 and 0.27 respectively; the lowest of all stocks previously introduced in Cuba. The above, together with the low amount of allelic variants detected for each microsatellite, was suggestive of low genetic variability. In addition, pairwise relatedness coefficients clustered around unity, indicating a high degree of consanguinity. Taken as a whole, the data suggests that this breeding stock should be crossed first with other individuals from a different source or with higher genetic variability.
REFERENCES
Tizol R, Jaime B, Laria R, Pérez L, Machado R, Silveira R. Introducción en Cuba del camarón blanco del pacifico L. vannamei. Etapa I Cuarentena. Repositorio digital Ocean Docs; 2004 [cited 2010 Nov 16]. Available from: http://hdl.handle.net/1834/3588 .
Borrell YJ, Espinosa G, Vazquez E, Sánchez JA, Blanco G. Variabilidad genética de loci microsatélites en los primeros lotes de Litopenaeus vannamei introducidos en Cuba para la acuicultura. Rev Invest Mar. 2006;27(3):237-44.
Machado TR. Assessment of genetic variability in two lots of white shrimp, Litopenaeus vannamei (Boone, 1931) introduced to Cuba. International Fischeries Management [Master dissertation]. Department of Aquatic Biosciences. Norwegian College of Fishery Science: University of Tronso, Norway; 2006.
Sbordoni V, de Matthaeis M, Cobolli Sbordoni M, La Rosa G, Mattoccia M. Bottleneck effects and the depression of genetic variability in hatchery stocks of Litopenaeus japonicus (Crustacea, Decapoda). Aquaculture. 1986;57:239-51.
Bierne N, Beuzart I, Vonau V, Bonhomme F, Bedier E. Microsatellite-associated heterosis in hatchery-propagated stocks of the shrimp Peneaus stylirostris. Aquaculture. 2000;184:203-19.
Xu Z, Primavera JH, de la Pena LD, Pettit P, Belak J, Alcivar-Warren A. Genetic diversity of wild and cultured Black Tiger Shrimp (Penaeus monodon) in the Philippines using microsatellites. Aquaculture 2001;199:13-40.
Garcia DK, Faggart MA, Rhoades L, Alcivar-Warren AA, Wyban JA, Carr WH, et al. Genetic diversity of cultured Penaeus vannamei shrimp using three molecular genetic techniques. Mol Mar Biol Biotech. 1994;3:270-80.
Wolfus GM, García GK, Alcivar-Warren A. Application of the microsatellite technique for analyzing genetic diversity in shrimp breeding programs. Aquaculture. 1997;152:35-47.
Benzie JAH. Population genetics structure in peneaid prawns. Aquaculture Res. 2000;31:95-119.
Cruz P, Mejía-Ruiz CH, Pérez-Enriquez R, Ibarra AM. Isolation and characterization of microsatellites in Pacific white shrimp Peneaus (Litopenaeus vannamei). Mol Ecol Notes. 2002;2:239-41.
Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 1978;89:583-90.
Peakall R, Smouse PE. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes. 2006;6:288-95.
Graur D, W-H Li. Fundamentals of Molecular Evolution. 2nd ed. Sunderland (MA): Sinauer Asociates; 2000.
Wright S. The interpretation of population structure by F-statistics with special regards to systems of mating. Evolution. 1965;19:395-420.
Goudet J. FSTAT software (version 2.9.3.2). Lausanne: University of Lausanne; 2002 [updated 2005 Aug 23, cited 2010 Oct 14]. Available from: http://www.unil.ch/popgen/softwares/fstat.htm .
Queller DC, Goodnight KF. Estimating relatedness using genetic markers. Evolution. 1989;43(2):258-75.
Cruz P, Ibarra AM, Mejia-Ruiz H, Gaffney PM, Perez-Enríquez R. Genetic variability assessed by microsatellites in a breeding program of Pacific White Shrimp (Litopenaeus vannamei). Mar Biotechnol. 2004;6:157-64.
Valles-Jimenez R, Cruz P, Perez-Enriquez R. Population genetic structure of Pacific white shrimp (Litopenaeus vannamei) from Mexico to Panama: microsatellite DNA variation. Mar Biotechnol (NY). 2004;6(5):475-84.
Pérez-Enríquez R, Hernández-Martínez F, Cruz P. Genetic diversity status of White shrimp Penaeus (Litopenaeus vannamei) broodstock in Mexico. Aquaculture. 2009; 297:44-50.
Freitas PD, Jesús CM, Galleti PM Jr. Isolation and characterization of new microsatellite loci in the Pacific white shrimp Litopenaeus vannamei and cross-species amplification in other penaeid species. Mol Ecol Notes. 2007;7(2):324-6.
Meehan D, Xu Z, Zuniga G, Alcivar-Warren A. High frequency and large number of polymorphic microsatellites in cultured shrimp, Penaeus (Litopenaeus vannamei) [Crustacea:Decapoda]. Mar Biotechnol (NY). 2003;5(4):311-30.
Borrell YJ, Álvarez J, Vázquez E, Fernández Pato C, Martínez Tapia C, Sánchez JA, et al. Applying microsatellites to the management of farmed turbot stocks (Scophthalmus maximus L.) in hatcheries. Aquaculture. 2004;241:133-50.