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Órgano oficial de difusión de la Federación Mexicana de Patología Clínica, AC y de la Asociación Latinoamericana de Patología Clínica/Medicina de Laboratorio

2012, Number 2

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Rev Mex Patol Clin Med Lab 2012; 59 (2)

Prevalence of diverse Candida species in women with cervical dysplasia in a Hospital of Puebla, Mexico

López-García A, Ruiz-Tagle A, Pérez-Tlacomulco A, Mauleón-Montero A, Sánchez-Hernández JA, Rivera-Tapia JA

Language: Spanish
References: 15
Page: 101-106
PDF size: 39.08 Kb.


ABSTRACT

Women with cervical dysplasia may be susceptible to disseminated infection with Candida since conditions are created to help these yeasts to proliferate easily. The aim of this study is to determine the prevalence of different Candida species in vaginal secretions of patients with dysplasia in a Puebla city hospital. Vaginal smears were made and deposited in saline solution and Stuart transportation broth. Saline solution samples were used to perform a direct smear where guide cells, Trichomonas, yeast, PMN and epithelial cells were searched. Stuart broth was used to perform a smear for Gram staining to search for yeast, Gram positive, Gram-negative bacilli, Gram-positive cocci, Gram-negative cocci and Gram-negative coccobacilli. Also to inoculate CHROMagar plates, which were incubated at 37 ±C from 24 to 72 h. When the incubation time had passed, the lecture of the plates was carried out to identify Candida species according to their appearance and color. From the three hundred cultures from patients with cervical dysplasia, we obtained 37% of positive cultures for pathogens and 63% normal flora. In positive cultures for microorganisms were identified the following pathogens Staphylococcus saprophyticus 23%, Candida 20%, Escherichia coli 20%, Streptococcus agalactiae 9%, Staphylococcus aureus 9% and Gardnerella vaginalis 3%. The vaginal smears diagnosed with cervical dysplasia obtained 32/300 positive cultures for Candida.


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