Juárez BA, Flores EXE, Serrano MNM, Cruz RE, Castellanos BG, Vega SR

Language: Spanish
References: 15
Page: 8-15
PDF size: 521.64 Kb.

ABSTRACT

Background: Cryopreservation permits to keep sperm cells for undefined time until the moment to be used. However, the process can damage them or even to kill them because of thermal changes.
Objective: Comparing sperm recovery of semen samples of fertile and infertile men after being subjected to a process of cryopreservation.
Method: Semen samples of 25 fertile men and 13 of infertile men were frozen by two weeks. Sperm concentration, mobility and morphology, as well as total of mobile cells (TCM) were compared before and after cryopreservation. Semen samples of fertile men were divided in 12 with normal semen values (FPN) and 13 with altered semen values (FPA). Mann-Whitney U test was used considering significant p‹0.05.
Results: There was a drop of mobility from 65.12% to 42.08% and TCM from 123.37 million to 62.05 million in samples of fertile men. In infertile men the mobility changed from 53.38% to 7.08% and TCM from 64.57 million to 11.75 million. In fertile men with altered values TCM is comparable to that of infertile men, but sperm recovery was much higher.
Recovery of frozen semen samples is about 50%, but only 15% in those of infertile men.
Conclusions: It is important to considerer sperm loss for deciding the number of semen samples to be frozen.

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