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TIP Revista Especializada en Ciencias Químico-Biológicas

2011, Number 1

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TIP Rev Esp Cienc Quim Biol 2011; 14 (1)

Pigmentos en los testículos de cinco anfibios endémicos de cuba (Eleutherodactylus turquinensis, E. cuneatus, E. glamyrus, Bufo longinasus longinasus y B. longinasus cajalbanensis)

Sanz-Ochotorena A, Segura-Valdés ML, Rodríguez-Gómez Y, Lara-Martínez R, Jiménez-García LF

Language: Spanish
References: 28
Page: 30-37
PDF size: 468.39 Kb.


ABSTRACT

Ectothermic vertebrates may have a pigment of unknown function, although in some cases melanomacrophage centers with apparent phagocytic function have been described; however, it is not common to find pigmented gonads in Anuran amphibians. The main objective of this paper is to report and analyze the presence of pigment in the testes of frogs (three species) and toads (one species), all of which are endemic to Cuba: Eleutherodactylus turquinensis, E. cuneatus, E. glamyrus, and Bufo longinasus (subspecies B. longinasus longinasus and B. longinasus cajalbanensis). The specimens were collected in western and eastern areas of the Island of Cuba and were anesthetized with ether once taken to the laboratory. Gonads were extracted and processed for light microscopy and, in the case of B. longinasus, also for transmission electron microscopy. The results show a morphology that is similar to that in other anuran testes; there was a cystic organization of sex cells in the seminiferous tubules and all animals were in total spermatogenesis. The testes of all studied species showed a dark and abundant pigmentation in the tunica albuginea and in the interstitial tissue. Many melanosomes were observed. In this paper, we analyze that the cause of strong testicular pigmentation may be a response to pollution and it is proposed that pigments are produced by another cell type similar to melanomacrophages, but not by melanocytes.


REFERENCES

  1. Gilbert, S.F. Developmental Biology (Ninth Edition, Sinauer Ass. Publishers, 2010) 711 págs.

  2. Kardong, K. Vertebrates: Comparative Anatomy, Function, Evolution (McGraw-Hill Science/Engineering/Math, 5 edition, 2008) 800 págs.

  3. Romer, A.S. & Parsons, T.S. The Vertebrate Body (Sixth edition, Saunders College Publishing, Philadelphia) (1986) 579 págs.

  4. Uribe-Arnazábal, M.C. Aparato Reproductor Masculino. En: Atlas de Histología de Vertebrados (comp. Estrada, E. & Uribe, M.C) 133-148 (2002).

  5. de Oliveira, C & Zieri, R. Testicular pigmentation in Physalaemus nattereri (Steindachner) (Amphibia, Anura) with anatomical obervations on the extracutaneus pigmentary system. Revista Brasilera de Zoologia 22, 454-460 (2005).

  6. Moresco, R.M. & de Oliveira, C. A Comparative study of the extracutaneous pigmentary system in three Anuran Amphibian species evaluated during the breeding season. South American Journal of Herpetology 4(1), 1-8 (2009).

  7. Scalia, M. et al. The spleen pigment in some Amphibia. Pigment Cell Research 17(2), 119-127 (2004).

  8. Zuasti, A., Jiménez-Cervantes, C., García Borrón & Ferreri, C. The melanogenic system of Xenopus laevis. Archives of Histology and Citology 61, 305-316 (1998).

  9. Sichel, G., Scalia, M., Mondio, F. & Corsaro, C. The amphibian Kupffer cells build and demolish melanosomes: an ultrastructural point of view. Pigment Cell Res 10, 271-287 (1997).

  10. Prelovsek, P.M. & Bulog, B. Biogenesis of melanosomes in Kupffer cells of Proteus anguinus (Urodela: Amphibia). Pigment Cell Research 16(4), 345-350 (2003).

  11. de Oliveira, C., Zanetoni, C. & Zieri, R. Morphological observations on the testes of Physalaemus cuvieri (Amphibia, Anura). Rev. Chil. Anat. 20(3), 263-268 (2002).

  12. Zieri, R., Taboga, S.R. & de Oliveira, C. Melanocytes in the testes of Eupemphix nattereri (Anura, Leiuperidae): Histological, stereological, and ultrastructural aspects. The Anatomical Record 290, 795-800 (2007).

  13. Franco-Belusi, L., Zieri, R., de Souza Santos, R., Moresco, R.M. & de Oliveira, C. Pigmentation in Anuran testes: Anatomical pattern and variation. The Anatomical Record 292, 178-182 (2009).

  14. Díaz, L.M. & Cádiz, A. Guía taxonómica de los anfibios de Cuba. Abc Taxa 4, i-vi, 294 págs. (2008).

  15. Vázquez-Nin, G.H. & Echevarría, O.M. Introducción a la Microscopía Aplicada a las Ciencias Biológicas (UNAM, Fondo de Cultura Económica, México, D.F., 2000).

  16. de Oliveira, C., Santanna, A.C., Omena, P.M., Santos, L.R.S. & Zieri, R. Morphological considerations on the seminiferous structures and testes of anuran amphibians: Bufo crucifer, Physalaemus cuvieri, and Scinax fuscovarius. Biociências 11(1), 39-46 (2003).

  17. Gallone, A., Guida, G., Maida, I. & Cicero, R. Spleen and liver pigmented macrophagues of Rana esculenta L. A new melanogenic system? Pigment Cell Research 15(1), 32-40 (2002).

  18. Duellman, W.E. & Trueb, L. Biology of Amphibian (New York, McGraw-Hill, 1994) 670 págs.

  19. de Souza Santos, L.R. & de Oliveira, C. Histological aspects and structural characteristics of the testes of Dendropsophus minutus (Anura, Hylidae). Micron doi:10.1016/j.micron. 2008.03.006 (2008).

  20. Rodríguez-Gómez, Y. Morfología de las gónadas de cuatro especies del género Eleutherodactylus (Anura. Leptodactylidae). Tesis doctoral. Universidad de la Habana. Cuba. 98 págs. (2008).

  21. Sanz-Ochotorena, A., Segura-Valdés, M.L., Rodríguez-Gómez,Y., Lara Martínez, R. & Jiménez-García, L.F. Estructura y ultraestructura de la gónada de los machos de Bufo fustiger (Anura: Bufonidae). TIP Rev. Esp. Cienc. Quím. Biol. 11(2), 81-86 (2008).

  22. Fowler, D.M. et al. Functional amyloid formation within mammalian tissue. PLoS Biol 4(1), e6 (2006).

  23. Schmid, M. et al. The Chromosomes of Terraranan Frogs. Insights into Vertebrate Cytogenetics (eds. Schmid, B. & Hedges S.B.) 559 págs. (2010).

  24. Díaz, L. & Cádiz, A. Pflege und Vermehrung von Bufo longinasus Stejneger, 1905: Ein Beitrag zur Erhaltung dieser Art. Aquaristik Fachmagazin and Aquarium heute 38(2), 18-21 (2006).

  25. Arencibia, G. et al. Evaluación de la contaminación de la Bahía de Guantánamo, Cuba. XXV Congreso de Ciencias del Mar y XI Congreso Latinoamericano de Ciencias del Mar. Viña del Mar, Chile (2005).

  26. Torres-Rodríguez, A., Puente-Lee, I., González, S. & Castrillón, M. Caracterización histológica y ultraestructural de los centros melanomacrófagos y su proliferación por efecto de los metales en hígado de Rana catesbeiana (Rana toro). Congreso Interamericano de Microscopía Electrónica. Rosario, Argentina. 23-26 (2009).

  27. Ogielska, M., Rozenblut, B., Augustyñska, R.& Kotusz, A. Degeneration of germ line cells in amphibian ovary. Acta Zoologica (Stockholm) 91, 319-327 (2010).

  28. Díaz, L.M., Cádiz, A., Chong, A. & Silva, A. First Report of Chytridiomycosis in a Dying Toad (Anura: Bufonidae) from Cuba: A New Conservation Challenge for the Island. EcoHealth 4, 172-175 (2007).




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