Immersion sterilization of laparoscopic surgery instruments. Comparative study among 2% glutaraldehyde, superoxidized water with neutral pH, and electrolyzed solution by ionic selectivity with neutral pH

Francisco J Nachón García; José Díaz Téllez; Víctor Rivas Espinoza; Jorge Sigfrido González H; Ma Gabriela Nachón G; Fabio García García; Juan Santiago García

2010, Number 4

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Cir Gen 2010; 32 (4)

Immersion sterilization of laparoscopic surgery instruments. Comparative study among 2% glutaraldehyde, superoxidized water with neutral pH, and electrolyzed solution by ionic selectivity with neutral pH

Nachón GFJ, Díaz TJ, Rivas ER, González HJS, Nachón GMG, García GF, Santiago GJ

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Language: Spanish
References: 26
Page: 226-231
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ABSTRACT

Objective: To compare the sterilizing efficacy in vitro of 2% glutaraldehyde (2% GA), of superoxidized electrolyzed water (SEW), and electrolyzed sterilizing solution by ionic activity (ESSIA).
Setting: University of Veracruz.
Design: Controlled clinical assay.
Statistical analysis: Chi square.
Material and methods: We prepared contaminating solutions with on E. coli, P. aeruginosa, and methicillin- resistant S. aureus, as well as with C. albicans at concentrations of 1 × 10⁸ CFU per milliliter in asterile saline solution, in which surgical instruments were immersed for 5 min and, thereafter, they were exposed to each of the sterilizing solutions for three different times, 7, 10, and 15 min.
Results: After 15 min of immersion in the sterilizing solutions, all cultures assessed in the instruments treated with 2% GA and ESSIA were negative, in contrast to four positive cultures found in the SEWtreated instruments (p ‹ 0.05).
Conclusion: Instruments treated with 2% GA and ESSIA comply with sterile requirements.

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