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2009, Number 4

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Bioquimia 2009; 34 (4)

Molecular and phenotypic characterization of A. hydrophila-like HG3 strain isolated of an infant with diarrhea in Mexico

Aguilera-Arreola MG, Hernández-Rodríguez CH, Castro-Escarpulli G

Language: English
References: 37
Page: 183-189
PDF size: 318.91 Kb.


ABSTRACT

A. hydrophila-like HG3 strain was the only enteric pathogen isolated from loose stools of a one-year-old infant from Acaxochitlán Hidalgo, Mexico. Isolation of the HG3 strain (phenotype A. hydrophila) from feces of patients with diarrhea has a low worldwide isolation frequency and this is the first report for Mexico. The strain was identified based on biochemical characteristics, RFLP 16S rDNA profile, and 16S rRNA gene sequence. The strain was characterized according to its ability to produce several virulence-related proteins and genes. Aerolysin/hemolysin, heat labile cytotonic enterotoxin (alt), lipases, including the glycerophospholipid-cholesterol acyltransferase (GCAT), and DNases were phenotypically and genetically detected. The heat-stable cytotonic enterotoxin (ast) and laf genes were not detected and the strain showed susceptibility to cephalosporin and quinolones. The virulence potential of this particular strain was documented and the clinical importance of similar strains must be made known. The apparently low frequency of the HG 3 strains should not be underestimated because those virulence factors are shared with other Aeromonas species that exhibit higher incidences. The present study adds new information to the knowledge on these particular strains.


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