Morphologic changes  induced by stress in  the population of Paneth cells

David Israel Martínez García; Jaime Camacho Aguilera; Iván Villanueva Becerril; Adriana Becerril Montes

2009, Number 3

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Rev Med Hosp Gen Mex 2009; 72 (3)

Morphologic changes induced by stress in the population of Paneth cells

Martínez GDI, Camacho AJ, Villanueva BI, Becerril MA

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Language: Spanish
References: 26
Page: 129-135
PDF size: 240.00 Kb.

ABSTRACT

Introduction: The mucous membrane of the small intestine forms villi that are covered by epithelium that is extended between the villi into the lamina propria to form the crypts of Lieberkühn. Paneth cells (PC) are found lying at the bottom of these crypts. They show acidophilic granules into their cytoplasm containing zinc, lysozymes, phospholipase A, IgA and other antimicrobial peptides. Induction of stress causes disturbances in physiological homeostasis that activate the hypothalamus-hypophysis-adrenal axis and the autonomic nervous system, thus resulting in corticoid and catecholamine release. Objective: To study the effects of stress on the morphology and population of the PC along the small intestine of adult rats. Material and methods: Eighteen Wistar male rats were divided in three groups, one control and two study groups in which a stressful forced swimming-condition, whether acute or chronic, was applied, and samples of the small intestine were obtained after perfusion with PFA. Five-micron slices were obtained from the tissue samples by histological processing, and stained by Gomori trichrome, wheat lectine or rodamine coupled- anti-alpha-defensin immunodetection. Slides were analyzed under optic microscope for PC number and granule content. Results: An increase in PC density and granule content was seen in stress-exposed groups, but not in control group. The increase in the number of cells can be interpreted as an effect of stress stimulating the maturation rate of PC, and the increase in granule content suggests that stress induces the functional activation of these cells. Conclusions: It is concluded that the PC population is affected by stress in a way that increases its activity.

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