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2009, Number 3

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Rev Med Hosp Gen Mex 2009; 72 (3)

Seroprevalence of Helicobacter pylori in patients with chronic gastritis from an urban zone in the State of Mexico

Paniagua-Contreras GL, Monroy-Pérez E, Arroniz-Padilla S, Hoyos-Torres L, Pineda-Sánchez MJ, Vaca-Pacheco S

Language: Spanish
References: 42
Page: 122-128
PDF size: 160.98 Kb.


ABSTRACT

The aim of this work was to determine the IgG-anti H. pylori -serum levels and the presence of the bacterium and its cytotoxic genotypes in gastric biopsies of 138 patients with chronic gastritis. Antibodies against H. pylori were quantified by ELISA. Gastric biopsies were inoculated in rich media and the plates were incubated in 6% CO2. H. pylori was identified by conventional PCR and the cytotoxic genotypes vacA and cagA by multiplex PCR. Most patients (94.9%) have had antibodies against H. pylori; among them 32.8% showed 20-60 AU/mL, 9.5% 61-99 AU/mL and 52.6% ›100 AU/mL. Sixty-two biopsies (45%) gave positive culture for H. pylori. All H. pylori strains were vacA+ but only 28 (45.2%) were cagA+. Genotypes of the analyzed strains were: vacAs1/vacAm1 (46.8%), vacAs1/vacAm2 (19.3%), vacs2/vacAm1 (25.8%), and vacAs2/vacAm2 (8%). Twenty nine percent of the strains showed the virulent genotype vacAs1/cagA. These results show that the patients suffering chronic gastritis we have studied had a high frequency of infection by H. pylori virulent strains, since two thirds of them (66%) had the vacAs1 allele, and approximately one third (29%) had both vacAs1 and cagA virulence markers.


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