medigraphic.com
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2008, Number 3

<< Back Next >>

Rev Biomed 2008; 19 (3)

Antibiotic resistance of Pseudomonas aeruginosa clinical isolates obtained from a university hospital in Lima, Perú

Luján-Roca DA, Ibarra-Trujillo JO, Mamani-Huamán E

Language: Spanish
References: 28
Page: 156-160
PDF size: 148.36 Kb.


ABSTRACT

Introduction. Pseudomonas aeruginosa is the cause of infections in immunosupressed patients and it is an important nosocomial pathogen.
Objective. To evaluate the antibiotic resistance of P. aeruginosa clinical isolates and to obtain data that can be useful in epidemiological studies.
Materials and methods. Between January and December 2003 a total of 144 Pseudomonas aeruginosa isolates were obtained on culture from patients of the National Hospital Hipólito Unanue (university hospital), Lima, Perú. The antibiotic susceptibility-resistance was evaluated by the disc diffusion Kirby-Bauer method for ceftazidime, cefepime, aztreonam, imipenem, meropenem, amikacin, gentamicin and ciprofloxacin.
Results. The antibiotics that showed higher resistance percentage were: ceftazidime (71%), aztreonam (62%), ciprofloxacin (57%) and gentamicin (55%). The antibiotic that showed better activity was meropenem (73% of sensitive strains).
Conclusions. High antibiotic resistance was found in P. auroginosa isolates obtained from patients of this hospital. The resistance figures were not uniform; therefore, it is suggested to carry out more antibiotic sensitivity-resistance assays to prescribe the appropriate antibiotic treatment as to limit the increase of resistant organisms.


REFERENCES

  1. Pollack M. P. aeruginosa. En: Mandell DaB, editor. Principles and practice of infectious diseases. 5th ed. New York: Churchill Livingstone; 2000. p. 2310-35.

  2. Kluytmans J. Surgical infections including burns. En: Wenzel RP, editor. Prevention and control of nosocomial infections. 3rd. ed. Baltimore: Williams & Wilkins; 1997. p. 841-65.

  3. Jones A, Dodd M, Doherty C, Govan J, Webb A. Increased treatment requirements of patients with cystic fibrosis who harbour highly transmissible strain of Pseudomonas aeruginosa. Thorax 2002; 57:924-5.

  4. Van Delden C, Iglewski B. Cell-to-cell signaling and Pseudomonas aeruginosa infections. Emerg Infect Dis 1998; 4:551-60.

  5. Gómez CA, Leal AL, Pérez MA, Navarrete ML. Mecanismos de resistencia en Pseudomonas aeruginosa: entendiendo a un peligroso enemigo. Rev Fac Med Univ Nac Colomb 2005; 53:27-34.

  6. Manual de procedimientos bacteriológicos en infecciones intrahospitalarias. Instituto Nacional de Salud. Ministerio de Salud. Lima. Perú. 2001.

  7. NCCLS 2002. Performance standards for antimicrobial disk susceptibility tests. 7th ed. Approved standard M02-A7. National Committee for Clinical Laboratory Standards, Villanova. PA. USA.

  8. Fluit AC, Schmitz FJ, Verhoef J, and the European SENTRY Participants Group. Frecuency of isolation of pathogens from bloodstream, nosocomial pneumonia, skin and soft tissue, and urinary tract infections occurring in european patients. Eur J Clin Infect Dis 2001; 20:188-91.

  9. Jarvis WR, Martone WJ. Predominant pathogens in hospitals infections. J Antimicrob Chemother 1992; 29 (Suppl A):19-24.

  10. Sader HS, Gales AC, Pfaller MA, Mendes RE, Zoccoli C, Barth A, et al. Pathogen frecuency and resistance patterns in Brazilian hospitals: summary of results from three years of SENTRY Antimicrobial Surveillance Program. Braz J Infect Dis 2001; 5:200-14.

  11. Lambert PA. Mechanism of antibiotic resistance in Pseudomonas aeruginosa. J R Soc Med 2002; 95:22-6.

  12. Livermore DM. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare?. Clin Infect Dis 2002; 34: 634-40.

  13. Nouér SA. Aspectos clínicos e fatores de risco relacionados com colonização ou infecção por Pseudomonas aeruginosa multirresistente. Universidade Federal do Rio de Janeiro. Río de Janeiro, Brasil. Tesis de doctorado. 2005.

  14. Nuñez L, Soto A, Calmet E, Castillo M, Casalindo E. Evaluación clínica y de laboratorio de las infecciones producidas por Pseudomonas aeruginosa en el hospital Arzobispo Loayza. Rev Per Enferm Infecc Trop 2001; 1: 180-3.

  15. Avellaneda JM, Pecho E. Estudio de la resistencia a los antibacterianos en el Centro Médico Naval de Enero a Diciembre del 2000. Universidad Nacional Mayor de San Marcos. Lima, Perú. Tesis para optar al título de Químico Farmacéutico. 2001.

  16. Pellegrino FL, Teixeira LM, Carvalho Md MG, Aranha NS, Pinto DO, Mello Sampaio JL, et al. Ocurrence of a multidrug-resistance Pseudomonas aeruginosa clone in different hospitals in Rio de Janeiro, Brazil. J Clin Microbiol 2002; 40: 2420-4.

  17. Brown PD, Izundu A. Antibiotic resistance in clinical isolates of Pseudomonas aeruginosa in Jamaica. Rev Panam Salud Publica 2004; 16: 125-30.

  18. Guerrero C, Cesteros R, Miranda A, Menasalvas A, Blázquez R, Segovia M. Sensibilidad a antimicrobianos de aislamientos clínicos de Pseudomonas aeruginosa en Murcia. Rev Esp Quimioterap 2003; 16: 444-9.

  19. Gomila B, Pardo FJ, Moreno R, Celades E, García A. Sensibilidad de aislamientos clínicos de Pseudomonas aeruginosa en Castellón. Rev Esp Quimioterap 2006; 19: 60-4.

  20. Cobo F, Bermúdez P, Manchado P. Situación actual de la resistencia de Pseudomonas aeruginosa a los antimicrobianos. Rev Esp Quimioterap 2003; 16:450-2.

  21. Van Eldere J. Multicentre surveillance of Pseudomonas aeruginosa susceptibility patterns in nosocomial infections. J Antimicrob Chemother 2003; 51:347-52.

  22. Livermore DM. Of Pseudomonas, porins, pumps and carbapenems [leading article]. J Antimicrob Chemother 2001; 47:247-50.

  23. Carmeli Y, Troillet N, Eliopoulos GM, Samore M. Emergence of antibiotic- resistant Pseudomonas aeruginosa: comparison of risk associated with different antipseudomonal agents. Antimicrob Agents Chemother 1999; 43:1379-82.

  24. Sader H. Resistencia antimicrobiana en Latinoamérica. ¿Cómo estamos?. Rev Chil Infect 2002; 19 (Suppl 1):5-13.

  25. Gales AC, Jones RN, Turnidge J, Rennie R, Ramphal R. Characterization of Pseudomonas aeruginosa isolates: occurrence rates, antimicrobial susceptibility patterns, and molecular typing in the global SENTRY antimicrobial surveillance program, 1997-1999. Clin Infect Dis 2001; 32 (Suppl 2):147-55.

  26. Gencer S, Ak O, Benzonana N, Bat rel A, Ozer S. Susceptibility patterns and cross resistance of antibiotics against Pseudomonas aeruginosa in a teaching hospital of Turkey. Ann Clin Microbiol Antimicrob 2002; 1:2.

  27. Naaber P, Koljalg S, Maimets M. Antibiotic usage and resistance-trends in Estonian university hospitals. Int J Antimicrob Agents 2000; 16:309-15.

  28. Sorberg M, Farra A, Ransjo U, Gardlund B, Rylander M, Settergren B, et al. Different trends in antibiotic resistance rates at a university teaching hospital. Clin Microbiol Infect 2003; 9:388-96.




2020     |     www.medigraphic.com