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2008, Number 4

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Rev Inst Nal Enf Resp Mex 2008; 21 (4)

Molecular basis of the regulation of water transport in lung and airways. Part one: Aquaporins

Salgado AAR, Navarro GMC

Language: Spanish
References: 34
Page: 263-271
PDF size: 144.05 Kb.


ABSTRACT

Water movement across epithelia is achieved through both the generation of osmotic gradients, and the presence of proteins that allow water to move freely across the lipid bilayer that makes up the plasma membrane of epithelial cells. In this two-part review, the molecular basis of water transport in the lung will be examined. The first part of the review will deal with water transport across aquaporin channels, while the second one will deal with the ion channels and pumps needed to create the osmotic gradient that allows water movement throughout the lung. Aquaporins are small, intrinsic membrane proteins that function as a water-permeable pore. They are functionally expressed throughout the organism, and in the lung they regulate the epithelial osmotic permeability and submucosal gland secretion. Recent research suggests an important role of lung-expressed aquaporins on the physiopathology of cystic fibrosis, pulmonary edema, bronchial hyperresponsiveness, infections and cancer, among other pathologies. Therefore, understanding the several functions aquaporins perform in the human lung might allow the development of new strategies for the management of the aforementioned pathologies.


REFERENCES

  1. Tripathi S, Boulpaep EL. Mechanisms of water transport by epithelial cells. Q J Exp Physiol 1989;74:385-417.

  2. Zeuthen T. General models for water transport across leaky epithelia. Int Rev Cytol 2002;215:285-317.

  3. Verkman AS. Role of aquaporins in lung liquid physiology. Respir Physiol Neurobiol 2007;159:324-330.

  4. Finkelstein A. Water movement through lipid bilayers, pores, and plasma membranes. Theory ald reality. Zn Distinguished Lecture Series of the Society of General Physiologists. USA, New York: John Wiley & Sons;1987.

  5. Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc Natl Acad Sci U S A 1991;88:11110-11114.

  6. Preston GM, Carroll TP, Guggino WB, Agre P. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 1992;256:385-387.

  7. Verkman AS, Mitra AK. Structure and function of aquaporin water channels. Am J Physiol Renal Physiol 2000;278:F13-F28.

  8. Verkman AS. More than just water channels: unexpected cellular roles of aquaporins. J Cell Sci 2005;118(Pt 15):3225-3232.

  9. Jung JS, Preston GM, Smith BL, Guggino WB, Agre P. Molecular structure of the water channel through aquaporin CHIP. The hourglass model. J Biol Chem 1994;269:14648-14654.

  10. Yasui M, Hazama A, Kwon TH, Nielsen S, Guggino WB, Agre P. Rapid gating and anion permeability of an intracellular aquaporin. Nature 1999;402:184-187.

  11. Elkjaer ML, Nejsum LN, Gresz V, et ál. Immunolocalization of aquaporin-8 in rat kidney, gastrointestinal tract, testis, and airways. Am J Physiol Renal Physiol 2001;281:F1047-F1057.

  12. King LS, Nielsen S, Agre P, Brown RH. Decreased pulmonary vascular permeability in aquaporin-1-null humans. Proc Natl Acad Sci U S A 2002;99:1059-1063.

  13. Nielsen S, King LS, Christensen BM, Agre P. Aquaporins in complex tissues. II. Subcellular distribution in respiratory and glandular tissues of rat. Am J Physiol 1997;273(5 Pt 1):C1549-C1561.

  14. Frigeri A, Gropper MA, Turck CW, Verkman AS. Immunolocalization of the mercurial-insensitive water channel and glycerol intrinsic protein in epithelial cell plasma membranes. Proc Natl Acad Sci U S A 1995;92: 4328-4331.

  15. King LS, Nielsen S, Agre P. Aquaporins in complex tissues. I. Developmental patterns in respiratory and glandular tissues of rat. Am J Physiol 1997;273(5 Pt 1):C1541-C1548.

  16. Funaki H, Yamamoto T, Koyama Y, et ál. Localization and expression of AQP5 in cornea, serous salivary glands, and pulmonary epithelial cells. Am J Physiol 1998;275(4 Pt 1):C1151-C1157.

  17. Dobbs LG, González R, Matthay MA, Carter EP, Allen L, Verkman AS. Highly water-permeable type I alveolar epithelial cells confer high water permeability between the airspace and vasculature in rat lung. Proc Natl Acad Sci U S A 1998;95:2991-2996.

  18. Verkman AS, Matthay MA, Song Y. Aquaporin water channels and lung physiology. Am J Physiol Lung Cell Mol Physiol 2000;278:L867-L879.

  19. King LS, Agre P. Pathophysiology of the aquaporin water channels. Annu Rev Physiol 1996;58:619-648.

  20. King LS, Yasui M, Agre P. Aquaporins in health and disease. Mol Med Today 2000;6:60-65.

  21. Agre P, Saboori AM, Asimos A, Smith BL. Purification and partial characterization of the Mr 30,000 integral membrane protein associated with the erythrocyte Rh(D) antigen. J Biol Chem 1987;262:17497-17503.

  22. Schreiber R, Nitschke R, Greger R, Kunzelmann K. The cystic fibrosis transmembrane conductance regulator activates aquaporin 3 in airway epithelial cells. J Biol Chem 1999;274:11811-11816.

  23. Sato K, Kobayashi K, Aida S, Tamai S. Bronchiolar expression of aquaporin-3 (AQP3) in rat lung and its dynamics in pulmonary oedema. Pflugers Arch 2004;449: 106-114.

  24. Song Y, Fukuda N, Bai C, Ma T, Matthay MA, Verkman AS. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. J Physiol 2000;525 Pt 3:771-779.

  25. Hales CA, Du HK, Volokhov A, Mourfarrej R, Quinn DA. Aquaporin channels may modulate ventilator-induced lung injury. Respir Physiol 2001;124:159-166.

  26. Krane CM, Fortner CN, Hand AR. Aquaporin 5-deficient mouse lungs are hyperresponsive to cholinergic stimulation. Proc Natl Acad Sci U S A 2001;98:14114-14119.

  27. Krane CM, Towne JE, Menon AG. Cloning and characterization of murine Aqp5: evidence for a conserved aquaporin gene cluster. Mamm Genome 1999;10: 498-505.

  28. Anderson SD, Schoeffel RE, Finney M. Evaluation of ultrasonically nebulised solutions for provocation testing in patients with asthma. Thorax 1983;38:284-291.

  29. Towne JE, Harrod KS, Krane CM, Menon AG. Decreased expression of aquaporin (AQP)1 and AQP5 in mouse lung after acute viral infection. Am J Respir Cell Mol Biol 2000;22:34-44.

  30. Ohinata A, Nagai K, Nomura J, et ál. Lipopolysaccharide changes the subcellular distribution of aquaporin 5 and increases plasma membrane water permeability in mouse lung epithelial cells. Biochem Biophys Res Commun 2005;326:521-526.

  31. Verkman AS, Hara-Chikuma M, Papadopoulos MC. Aquaporins—new players in cancer biology. J Mol Med 2008;86:523-529.

  32. Saadoun S, Papadopoulos MC, Hara-Chikuma M, Verkman AS. Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption. Nature 2005;434:786-792.

  33. Hoque MO, Soria JC, Woo J, et ál. Aquaporin 1 is overexpressed in lung cancer and stimulates NIH-3T3 cell proliferation and anchorage-independent growth. Am J Pathol 2006;168:1345-1353.

  34. Chae YK, Woo J, Kim MJ, et ál. Expression of aquaporin 5 (AQP5) promotes tumor invasion in human non small cell lung cancer. PLoS ONE 2008;3:e2162.




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