Santoyo G

Language: Spanish
References: 64
Page: 38-47
PDF size: 157.25 Kb.

ABSTRACT

Recombineering is a powerful methodology employed to modify the bacterial DNA, either of chromosomal or plasmidic origin. This new tool utilizes the «Red» functions of the phage λ to carry on homologous recombination events with short homologies (~35-50 bp). In contrast to standard genetic engineering techniques, recombineering does not employ restriction enzymes and DNA ligases. Besides, the use of PCR products, as well as synthetic oligonucleotides, offers a wonderful advantage to modify any region of the bacterial genome. This review describes the homologous recombination mechanisms in E. coli and the Red functions of the phage λ. It also analyzes some of the uses and advantages of recombineering in Gram-negative and Gram-positive bacteria. Without a doubt, recombineering is changing the manner to engineer DNA in a precise, fast, efficient and economic way.

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