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2008, Number 2

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Bioquimia 2008; 33 (2)

Frequency and characterization of vapD gene in Helicobacter pylori strains of different vacA and cag-PAI genotype

Morales-Espinosa R, González-Valencia G, Delgado G, Méndez JL, Torres J, Cravioto A

Language: English
References: 38
Page: 43-50
PDF size: 173.33 Kb.


ABSTRACT

Several studies using different molecular-typing techniques have demonstrated that the gene content and the chromosomal arrangement among Helicobacter pylori strains are highly variable. Furthermore, this variability has been observed in different genes, such as vacA, which has a mosaic structure, and in cag-PAI, which has a different chromosomal organization. Preliminary study suggests that vapD is a strain-variable gene and it is present in about 60% of H. pylori strains. vapD gene is closely related to the virulence-associated protein D gene of Dichelobacter nodosus. The aim of this study was to characterize and to determine the frequency of vapD gene, in 301 Helicobacter pylori strains with different vacA and cag-PAI genotypes isolated from 27 Mexican patients with different gastric pathologies. The results showed that 70% of the patients presented multiple infections with H. pylori strains of different, vacA, and cag-PAI genotypes. Fifty two percent of the patients were colonized with vapD-positive and -negative strains. Regarding the vapD gene characterization by individual strains, it presented a frequency of 38%. This is the first characterization report that shows the frequency of vapD gene in H. pylori strains from Mexican patients. With respect to vacA and cag-PAI, the s1-m1/cag-PAI positive genotypes were more frequent in strains isolated from patients with recurrent abdominal pain (RAP), gastric ulcer (GU), and duodenal ulcer (DU). Statistical analysis showed that the vapD gene did not have association with any specific family of vacA or cag-PAI genotypes and specific gastric pathology.


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